0 10 Inhibition Inhibition NNP 11 13 of of IN 14 27 transcription transcription NN 28 34 factor factor NN 35 40 Stat1 stat1 NN 41 49 activity activity NN 50 52 in in IN 53 64 mononuclear mononuclear JJ 65 69 cell cell NN 70 78 cultures culture NNS 79 82 and and CC 83 84 T t NN 85 90 cells cell NNS 91 93 by by IN 94 97 the the DT 98 104 cyclic cyclic JJ 105 108 AMP amp NN 109 118 signaling signaling NN 119 126 pathway pathway NN 126 127 . . . 129 139 Activation Activation NNP 140 142 of of IN 143 144 T t NN 145 150 cells cell NNS 151 158 results result VBZ 159 161 in in IN 162 163 a a DT 164 171 cascade cascade NN 172 174 of of IN 175 179 gene gene NN 180 190 activation activation NN 191 194 and and CC 195 205 subsequent subsequent JJ 206 219 proliferation proliferation NN 220 223 and and CC 224 239 differentiation differentiation NN 240 244 into into IN 245 253 effector effector NN 254 264 phenotypes phenotype NNS 264 265 . . . 266 269 The the DT 270 280 regulation regulation NN 281 283 of of IN 284 297 transcription transcription NN 298 305 factors factor NNS 306 315 belonging belong VBG 316 318 to to TO 319 322 the the DT 323 329 signal signal NN 330 340 transducer transducer NN 341 344 and and CC 345 354 activator activator NN 355 357 of of IN 358 371 transcription transcription NN 372 373 ( ( ( 373 377 STAT STAT NNP 377 378 ) ) ) 379 385 family family NN 386 389 was be VBD 390 398 analyzed analyze VBN 399 401 in in IN 402 415 PHA-activated pha-activated JJ 416 427 mononuclear mononuclear JJ 428 433 cells cell NNS 434 437 and and CC 438 440 in in IN 441 449 purified purify VBN 450 451 T t NN 452 457 cells cell NNS 458 467 activated activate VBN 468 470 by by IN 471 484 cross-linking cross-linking JJ 485 489 cell cell NN 490 497 surface surface NN 498 501 CD3 cd3 NN 501 502 . . . 503 507 Cell cell NN 508 518 activation activation NN 519 527 resulted result VBD 528 530 in in IN 531 532 a a DT 533 540 delayed delay VBN 541 550 induction induction NN 551 553 of of IN 554 558 STAT STAT NNP 559 570 DNA-binding dna-binding JJ 571 579 activity activity NN 579 580 , , , 581 586 which which WDT 587 590 was be VBD 591 600 sustained sustained JJ 601 604 for for IN 605 612 several several JJ 613 617 days day NNS 617 618 , , , 619 622 was be VBD 623 631 composed compose VBN 632 645 predominantly predominantly RB 646 648 of of IN 649 654 Stat1 Stat1 NNP 655 658 and and CC 659 664 Stat3 Stat3 NNP 664 665 , , , 666 669 and and CC 670 673 was be VBD 674 681 blocked block VBN 682 684 by by IN 685 698 cycloheximide cycloheximide NN 699 702 and and CC 703 714 actinomycin actinomycin NN 715 717 D. D. NNP 718 727 Increased Increased NNP 728 733 Stat1 Stat1 NNP 734 737 and and CC 738 743 Stat3 Stat3 NNP 744 748 mRNA mRNA NNP 749 752 and and CC 753 760 protein protein NN 761 767 levels level NNS 768 772 were be VBD 773 781 detected detect VBN 781 782 , , , 783 795 respectively respectively RB 796 797 4 4 CD 798 801 and and CC 802 804 24 24 CD 805 806 h h NN 807 812 after after IN 813 823 activation activation NN 823 824 . . . 825 836 Stimulation stimulation NN 837 839 of of IN 840 843 the the DT 844 848 cAMP camp NN 849 855 signal signal NN 856 868 transduction transduction NN 869 876 pathway pathway NN 876 877 , , , 878 883 which which WDT 884 889 skews skew VBZ 890 898 cytokine cytokine NN 899 909 production production NN 910 916 toward toward IN 917 918 a a DT 919 922 Th2 th2 NN 923 930 pattern pattern NN 930 931 , , , 932 940 resulted result VBD 941 943 in in IN 944 947 the the DT 948 960 preferential preferential JJ 961 972 suppression suppression NN 973 975 of of IN 976 981 Stat1 Stat1 NNP 982 991 activity. activity. NN 992 996 cAMP cAMP NNP 997 1006 inhibited inhibit VBD 1007 1010 the the DT 1011 1020 induction induction NN 1021 1023 of of IN 1024 1034 expression expression NN 1035 1037 of of IN 1038 1042 IL-2 il-2 NN 1043 1051 receptor receptor NN 1052 1062 components component NNS 1062 1063 , , , 1064 1067 but but CC 1068 1071 did do VBD 1072 1075 not not RB 1076 1083 inhibit inhibit VB 1084 1088 IL-4 il-4 NN 1089 1097 receptor receptor NN 1098 1109 alpha-chain alpha-chain NN 1110 1113 and and CC 1114 1118 CD69 cd69 NN 1119 1129 expression expression NN 1130 1132 or or CC 1133 1136 the the DT 1137 1146 induction induction NN 1147 1149 of of IN 1150 1159 activator activator NN 1160 1167 protein protein NN 1168 1169 1 1 CD 1170 1183 transcription transcription NN 1184 1192 factors. factors. NN 1193 1197 cAMP camp NN 1198 1207 signaling signaling NN 1208 1217 inhibited inhibit VBD 1218 1223 Stat1 stat1 NN 1224 1226 at at IN 1227 1234 several several JJ 1235 1244 different different JJ 1245 1251 levels level NNS 1251 1252 , , , 1253 1262 including include VBG 1263 1274 suppression suppression NN 1275 1277 of of IN 1278 1281 DNA DNA NNP 1282 1289 binding binding NN 1290 1293 and and CC 1294 1309 down-regulation down-regulation NN 1310 1312 of of IN 1313 1318 Stat1 stat1 NN 1319 1326 protein protein NN 1327 1330 and and CC 1331 1335 mRNA mrna NN 1336 1342 levels level NNS 1342 1343 . . . 1344 1347 Our our PRP$ 1348 1355 results result NNS 1356 1367 demonstrate demonstrate VBP 1368 1371 the the DT 1372 1382 regulation regulation NN 1383 1385 of of IN 1386 1390 STAT STAT NNP 1391 1399 activity activity NN 1400 1402 by by IN 1403 1404 a a DT 1405 1414 signaling signaling NN 1415 1422 pathway pathway NN 1423 1427 that that WDT 1428 1437 regulates regulate VBZ 1438 1441 the the DT 1442 1443 T t NN 1444 1448 cell cell NN 1449 1459 functional functional JJ 1460 1469 phenotype phenotype NN 1470 1473 and and CC 1474 1476 is be VBZ 1477 1485 distinct distinct JJ 1486 1490 from from IN 1491 1494 the the DT 1495 1513 cytokine-activated cytokine-activated JJ 1514 1519 Janus Janus NNP 1520 1531 kinase-STAT kinase-stat NN 1532 1541 signaling signaling NN 1542 1549 pathway pathway NN 1549 1550 . . .