0 15 Transcriptional transcriptional JJ 16 24 analysis analysis NN 25 27 of of IN 28 40 Epstein-Barr epstein-barr JJ 41 46 virus virus NN 47 51 gene gene NN 52 62 expression expression NN 63 65 in in IN 66 78 EBV-positive ebv-positive JJ 79 86 gastric gastric JJ 87 96 carcinoma carcinoma NN 96 97 : : : 98 104 unique unique JJ 105 110 viral viral JJ 111 118 latency latency NN 119 121 in in IN 122 125 the the DT 126 132 tumour tumour NN 133 138 cells cell NNS 138 139 . . . 141 149 Although although IN 150 163 case-oriented case-oriented JJ 164 172 evidence evidence NN 173 176 for for IN 177 179 an an DT 180 191 association association NN 192 194 of of IN 195 207 Epstein-Barr epstein-barr JJ 208 213 virus virus NN 214 215 ( ( ( 215 218 EBV EBV NNP 218 219 ) ) ) 220 224 with with IN 225 232 gastric gastric JJ 233 242 carcinoma carcinoma NN 243 246 has have VBZ 247 251 been be VBN 252 264 accumulating accumulate VBG 265 273 recently recently RB 273 274 , , , 275 278 the the DT 279 293 interaction(s) interaction(s) NN 294 301 between between IN 302 305 EBV EBV NNP 306 309 and and CC 310 317 gastric gastric JJ 318 328 epithelial epithelial JJ 329 334 cells cell NNS 335 341 is/are is/are VBP 342 349 largely largely RB 350 357 unknown unknown JJ 357 358 . . . 359 361 In in IN 362 366 this this DT 367 372 study study NN 372 373 , , , 374 376 we we PRP 377 385 examined examine VBD 386 391 seven seven CD 392 404 EBV-positive ebv-positive JJ 405 412 gastric gastric JJ 413 422 carcinoma carcinoma NN 423 430 tissues tissue NNS 431 434 for for IN 435 440 viral viral JJ 441 445 gene gene NN 446 456 expression expression NN 457 459 at at IN 460 463 the the DT 464 468 mRNA mrna NN 469 474 level level NN 474 475 , , , 476 480 from from IN 481 486 which which WDT 487 494 studies study NNS 495 497 on on IN 498 501 the the DT 502 505 EBV EBV NNP 506 518 oncogenicity oncogenicity NN 519 521 in in IN 522 527 human human JJ 528 538 epithelial epithelial JJ 539 544 cells cell NNS 545 549 will will MD 550 557 benefit benefit VB 557 558 . . . 559 566 Reverse reverse JJ 567 584 transcription-PCR transcription-PCR NNP 585 593 analysis analysis NN 594 600 showed show VBD 601 605 that that IN 606 609 all all DT 610 615 seven seven CD 616 628 EBV-positive ebv-positive JJ 629 635 tumour tumour NN 636 643 tissues tissue NNS 644 658 constitutively constitutively RB 659 668 expressed express VBD 669 672 EBV EBV NNP 673 680 nuclear nuclear JJ 681 688 antigen antigen NN 689 690 ( ( ( 690 694 EBNA EBNA NNP 694 695 ) ) ) 696 697 1 1 CD 698 702 mRNA mrna NN 702 703 , , , 704 707 but but CC 708 711 not not RB 712 717 EBNA2 ebna2 NN 718 722 mRNA mRNA NNP 722 723 . . . 724 727 The the DT 728 732 EBNA EBNA NNP 733 746 transcription transcription NN 747 750 was be VBD 751 760 initiated initiate VBN 761 765 from from IN 766 769 one one CD 770 772 of of IN 773 778 three three CD 779 783 EBNA EBNA NNP 784 793 promoters promoter NNS 793 794 , , , 795 797 Qp Qp NNP 797 798 : : : 799 801 by by IN 802 810 contrast contrast NN 810 811 , , , 812 816 both both CC 817 819 Cp Cp NNP 820 823 and and CC 824 826 Wp Wp NNP 827 831 were be VBD 832 838 silent silent JJ 838 839 , , , 840 844 thus thus RB 845 854 resulting result VBG 855 857 in in IN 858 861 the the DT 862 866 lack lack NN 867 869 of of IN 870 875 EBNA2 EBNA2 NNP 876 880 mRNA mRNA NNP 880 881 . . . 882 888 Latent latent JJ 889 897 membrane membrane NN 898 905 protein protein NN 906 907 ( ( ( 907 910 LMP LMP NNP 910 911 ) ) ) 912 914 2A 2a NN 915 919 mRNA mrna NN 920 923 was be VBD 924 932 detected detect VBN 933 935 in in IN 936 941 three three CD 942 944 of of IN 945 950 seven seven CD 951 956 cases case NNS 956 957 ; ; : 958 965 however however RB 965 966 , , , 967 974 neither neither CC 975 979 LMP1 LMP1 NNP 980 983 nor nor CC 984 989 LMP2B LMP2B NNP 990 994 mRNA mRNA NNP 995 998 was be VBD 999 1007 detected detect VBN 1008 1010 in in IN 1011 1014 any any DT 1015 1017 of of IN 1018 1021 the the DT 1022 1029 tumours tumour NNS 1030 1036 tested test VBN 1036 1037 . . . 1038 1049 Transcripts transcript NNS 1050 1054 from from IN 1055 1058 the the DT 1059 1066 BamHI-A BamHI-A NNP 1067 1073 region region NN 1074 1076 of of IN 1077 1080 the the DT 1081 1086 viral viral JJ 1087 1093 genome genome NN 1094 1098 were be VBD 1099 1109 detectable detectable JJ 1110 1112 in in IN 1113 1116 all all DT 1117 1122 cases case NNS 1122 1123 . . . 1124 1129 BZLF1 BZLF1 NNP 1130 1134 mRNA mRNA NNP 1135 1138 and and CC 1139 1142 the the DT 1143 1150 product product NN 1150 1151 , , , 1152 1154 an an DT 1155 1170 immediate-early immediate-early JJ 1171 1175 gene gene NN 1176 1179 for for IN 1180 1183 EBV EBV NNP 1184 1195 replication replication NN 1195 1196 , , , 1197 1200 was be VBD 1201 1204 not not RB 1205 1214 expressed express VBN 1215 1217 in in IN 1218 1221 any any DT 1222 1224 of of IN 1225 1229 them them PRP 1229 1230 , , , 1231 1238 thereby thereby RB 1239 1249 suggesting suggest VBG 1250 1254 that that IN 1255 1258 the the DT 1259 1265 tumour tumour NN 1266 1271 cells cell NNS 1272 1279 carried carry VBD 1280 1283 EBV EBV NNP 1284 1291 genomes genome NNS 1292 1294 in in IN 1295 1296 a a DT 1297 1304 tightly tightly RB 1305 1311 latent latent JJ 1312 1316 form form NN 1316 1317 . . . 1318 1323 These these DT 1324 1332 findings finding NNS 1333 1340 further further RB 1341 1349 extended extend VBD 1350 1353 our our PRP$ 1354 1362 previous previous JJ 1363 1367 data datum NNS 1368 1377 regarding regard VBG 1378 1381 EBV EBV NNP 1382 1389 latency latency NN 1390 1392 in in IN 1393 1400 gastric gastric JJ 1401 1410 carcinoma carcinoma NN 1411 1416 cells cell NNS 1417 1419 at at IN 1420 1423 the the DT 1424 1431 protein protein NN 1432 1437 level level NN 1437 1438 , , , 1439 1442 and and CC 1443 1447 have have VBP 1448 1456 affirmed affirm VBN 1457 1461 that that IN 1462 1465 the the DT 1466 1475 programme programme NN 1476 1478 of of IN 1479 1484 viral viral JJ 1485 1489 gene gene NN 1490 1500 expression expression NN 1501 1503 in in IN 1504 1507 the the DT 1508 1514 tumour tumour NN 1515 1519 more more RBR 1520 1527 closely closely RB 1528 1537 resembles resemble VBZ 1538 1539 ‘ ` `` 1539 1546 latency latency NN 1547 1548 I i CD 1548 1549 ’ ' '' 1550 1561 represented represent VBN 1562 1564 by by IN 1565 1572 Burkitt Burkitt NNP 1572 1574 ’s 's POS 1575 1583 lymphoma lymphoma NN 1584 1588 than than IN 1589 1590 ‘ ` `` 1590 1597 latency latency NN 1598 1600 II ii CD 1600 1601 ’ ' '' 1602 1613 represented represent VBN 1614 1616 by by IN 1617 1620 the the DT 1621 1629 majority majority NN 1630 1632 of of IN 1633 1647 nasopharyngeal nasopharyngeal JJ 1648 1658 carcinomas carcinoma NNS 1658 1659 . . .