0 10 Attenuated attenuate VBN 11 19 function function NN 20 22 of of IN 23 24 a a DT 25 32 variant variant JJ 33 37 form form NN 38 40 of of IN 41 44 the the DT 45 61 helix-loop-helix helix-loop-helix JJ 62 69 protein protein NN 69 70 , , , 71 75 Id-3 Id-3 NNP 75 76 , , , 77 86 generated generate VBN 87 89 by by IN 90 92 an an DT 93 104 alternative alternative JJ 105 113 splicing splicing NN 114 123 mechanism mechanism NN 123 124 . . . 126 129 The the DT 130 132 Id id NN 133 139 family family NN 140 142 of of IN 143 159 helix-loop-helix helix-loop-helix JJ 160 168 proteins protein NNS 169 177 function function VBP 178 180 as as IN 181 189 negative negative JJ 190 200 regulators regulator NNS 201 203 of of IN 204 207 DNA DNA NNP 208 215 binding binding NN 215 216 , , , 217 222 basic basic JJ 223 239 helix-loop-helix helix-loop-helix JJ 240 248 proteins protein NNS 249 251 in in IN 252 255 the the DT 256 266 regulation regulation NN 267 269 of of IN 270 274 cell cell NN 275 281 growth growth NN 282 285 and and CC 286 301 differentiation differentiation NN 301 302 . . . 303 305 We we PRP 306 312 report report VBP 313 317 here here RB 318 320 on on IN 321 324 the the DT 325 339 identification identification NN 340 342 of of IN 343 344 a a DT 345 347 17 17 CD 348 351 kDa kda NN 352 359 variant variant NN 360 362 of of IN 363 366 the the DT 367 369 14 14 CD 370 373 kDa kda NN 374 378 Id-3 id-3 NN 379 386 protein protein NN 387 393 termed term VBN 394 399 Id-3L Id-3L NNP 400 401 ( ( ( 401 405 long long JJ 406 413 version version NN 413 414 ) ) ) 415 420 which which WDT 421 430 possesses possess VBZ 431 432 a a DT 433 439 unique unique JJ 440 442 60 60 CD 443 448 amino amino NN 449 453 acid acid NN 454 470 carboxy-terminus carboxy-terminus NN 471 480 generated generate VBN 481 483 by by IN 484 488 read read VBN 489 496 through through IN 497 499 of of IN 500 501 a a DT 502 503 ‘ ` `` 503 509 coding cod VBG 510 516 intron intron NN 516 517 ’ ' '' 518 521 and and CC 522 533 alternative alternative JJ 534 542 splicing splicing NN 542 543 . . . 544 552 Northern Northern NNP 553 561 analysis analysis NN 562 570 revealed reveal VBD 571 581 expression expression NN 582 584 of of IN 585 586 a a DT 587 592 minor minor JJ 593 596 1.1 1.1 CD 597 599 kb kb NN 600 605 Id-3L Id-3L NNP 606 616 transcript transcript NN 617 625 together together RB 626 630 with with IN 631 634 the the DT 635 646 predominant predominant JJ 647 651 0.95 0.95 CD 652 654 kb kb NN 655 659 Id-3 id-3 NN 660 670 transcript transcript NN 671 673 in in IN 674 677 the the DT 678 686 majority majority NN 687 689 of of IN 690 695 adult adult JJ 696 701 human human JJ 702 709 tissues tissue NNS 710 718 analysed analyse VBN 718 719 . . . 720 723 The the DT 724 731 variant variant JJ 732 737 Id-3L Id-3L NNP 738 745 protein protein NN 746 748 is be VBZ 749 761 functionally functionally RB 762 777 distinguishable distinguishable JJ 778 782 from from IN 783 795 conventional conventional JJ 796 800 Id-3 id-3 NN 801 806 since since IN 807 809 in in IN 810 812 in in FW 813 818 vitro vitro FW 819 822 DNA dna NN 823 831 mobility mobility NN 832 837 shift shift NN 838 844 assays assay NNS 844 845 , , , 846 848 it it PRP 849 852 was be VBD 853 860 greatly greatly RB 861 869 impaired impaired JJ 870 872 in in IN 873 876 its its PRP$ 877 884 ability ability NN 885 887 to to TO 888 896 abrogate abrogate VB 897 904 binding binding NN 905 907 of of IN 908 911 the the DT 912 917 basic basic JJ 918 934 helix-loop-helix helix-loop-helix JJ 935 942 protein protein NN 942 943 , , , 944 947 E47 E47 NNP 947 948 , , , 949 951 to to TO 952 954 an an DT 955 956 E e NN 957 960 box box NN 961 972 recognition recognition NN 973 981 sequence sequence NN 981 982 . . .