0 10 Regulation regulation NN 11 13 of of IN 14 26 sialoadhesin sialoadhesin NN 27 37 expression expression NN 38 40 on on IN 41 44 rat rat NN 45 56 macrophages macrophage NNS 56 57 . . . 58 67 Induction Induction NNP 68 70 by by IN 71 86 glucocorticoids glucocorticoid NNS 87 90 and and CC 91 102 enhancement enhancement NN 103 105 by by IN 106 114 IFN-beta IFN-beta NNP 114 115 , , , 116 125 IFN-gamma IFN-gamma NNP 125 126 , , , 127 131 IL-4 il-4 NN 131 132 , , , 133 136 and and CC 137 155 lipopolysaccharide lipopolysaccharide NN 155 156 . . . 158 170 Sialoadhesin Sialoadhesin NNP 171 173 is be VBZ 174 175 a a DT 176 197 macrophage-restricted macrophage-restricted JJ 198 204 member member NN 205 207 of of IN 208 211 the the DT 212 214 Ig ig NN 215 226 superfamily superfamily NN 227 231 that that WDT 232 240 mediates mediate VBZ 241 249 adhesion adhesion NN 250 254 with with IN 255 263 lymphoid lymphoid JJ 264 267 and and CC 268 275 myeloid myeloid JJ 276 281 cells cell NNS 281 282 . . . 283 285 It it PRP 286 288 is be VBZ 289 298 expressed express VBN 299 301 on on IN 302 303 a a DT 304 317 subpopulation subpopulation NN 318 320 of of IN 321 332 macrophages macrophage NNS 333 335 in in IN 336 344 lymphoid lymphoid JJ 345 352 tissues tissue NNS 353 356 and and CC 357 359 in in IN 360 367 chronic chronic JJ 368 380 inflammation inflammation NN 381 382 ( ( ( 382 386 e.g. e.g. FW 386 387 , , , 388 394 during during IN 395 405 autoimmune autoimmune JJ 406 414 diseases disease NNS 414 415 ) ) ) 415 416 . . . 417 419 We we PRP 420 424 have have VBP 425 432 studied study VBN 433 436 the the DT 437 447 regulation regulation NN 448 450 of of IN 451 463 sialoadhesin sialoadhesin NN 464 474 expression expression NN 475 477 in in FW 478 483 vitro vitro FW 484 487 and and CC 488 492 show show VBP 493 497 that that IN 498 513 glucocorticoids glucocorticoid NNS 514 515 ( ( ( 515 517 GC GC NNP 517 518 ) ) ) 519 525 induce induce VBP 526 538 sialoadhesin sialoadhesin NN 539 549 expression expression NN 550 552 on on IN 553 560 freshly freshly RB 561 569 isolated isolate VBN 570 573 rat rat NN 574 585 macrophages macrophage NNS 586 589 and and CC 590 593 the the DT 594 597 rat rat NN 598 608 macrophage macrophage NN 609 613 cell cell NN 614 618 line line NN 619 621 R2 r2 NN 621 622 . . . 623 626 The the DT 627 636 cytokines cytokine NNS 637 645 IFN-beta IFN-beta NNP 645 646 , , , 647 656 IFN-gamma IFN-gamma NNP 656 657 , , , 658 662 IL-4 il-4 NN 662 663 , , , 664 667 and and CC 668 671 LPS LPS NNP 671 672 , , , 673 681 although although IN 682 688 unable unable JJ 689 691 to to TO 692 698 induce induce VB 699 711 sialoadhesin sialoadhesin NN 712 722 expression expression NN 723 725 by by IN 726 736 themselves themselves PRP 736 737 , , , 738 742 were be VBD 743 747 able able JJ 748 750 to to TO 751 758 enhance enhance VB 759 770 GC-mediated gc-mediated JJ 771 780 induction induction NN 781 783 of of IN 784 796 sialoadhesin sialoadhesin NN 796 797 . . . 798 810 Sialoadhesin Sialoadhesin NNP 811 821 expression expression NN 822 825 was be VBD 826 836 functional functional JJ 837 839 as as IN 840 845 shown show VBN 846 848 by by IN 849 853 cell cell NN 854 862 adhesion adhesion NN 863 869 assays assay NNS 870 874 with with IN 875 880 human human JJ 881 885 RBCs rbc NNS 885 886 . . . 887 895 Northern Northern NNP 896 904 blotting blotting NN 905 916 experiments experiment NNS 917 926 indicated indicate VBD 927 931 that that IN 932 942 regulation regulation NN 943 956 predominantly predominantly RB 957 965 occurred occur VBD 966 968 at at IN 969 972 the the DT 973 977 mRNA mrna NN 978 983 level level NN 983 984 . . . 985 995 Comparison comparison NN 996 998 of of IN 999 1002 the the DT 1003 1012 different different JJ 1013 1025 combinations combination NNS 1026 1028 of of IN 1029 1031 GC GC NNP 1032 1035 and and CC 1036 1049 cytokines/LPS cytokines/lps NN 1050 1058 revealed reveal VBD 1059 1070 differences difference NNS 1071 1073 in in IN 1074 1077 the the DT 1078 1083 level level NN 1084 1086 of of IN 1087 1099 GC-dependent gc-dependent JJ 1100 1111 enhancement enhancement NN 1112 1114 of of IN 1115 1127 sialoadhesin sialoadhesin NN 1128 1138 expression expression NN 1138 1139 , , , 1140 1144 with with IN 1145 1153 IFN-beta IFN-beta NNP 1154 1157 and and CC 1158 1162 IL-4 il-4 NN 1163 1168 being be VBG 1169 1173 more more RBR 1174 1180 potent potent JJ 1181 1185 than than IN 1186 1195 IFN-gamma IFN-gamma NNP 1196 1199 and and CC 1200 1203 LPS LPS NNP 1203 1204 . . . 1205 1213 Moreover moreover RB 1213 1214 , , , 1215 1218 the the DT 1219 1226 effects effect NNS 1227 1229 of of IN 1230 1239 IFN-gamma IFN-gamma NNP 1240 1243 and and CC 1244 1247 LPS LPS NNP 1248 1253 could could MD 1254 1256 be be VB 1257 1267 reproduced reproduce VBN 1268 1270 by by IN 1271 1278 priming priming NN 1278 1279 , , , 1280 1287 whereas whereas IN 1288 1296 IFN-beta IFN-beta NNP 1297 1300 and and CC 1301 1305 IL-4 il-4 NN 1306 1310 were be VBD 1311 1319 required require VBN 1320 1334 simultaneously simultaneously RB 1335 1339 with with IN 1340 1342 GC GC NNP 1342 1343 . . . 1344 1347 The the DT 1348 1358 regulation regulation NN 1359 1361 of of IN 1362 1374 sialoadhesin sialoadhesin NN 1375 1385 expression expression NN 1386 1389 was be VBD 1390 1398 mediated mediate VBN 1399 1401 by by IN 1402 1405 the the DT 1406 1408 GC GC NNP 1409 1417 receptor receptor NN 1417 1418 , , , 1419 1422 and and CC 1423 1426 not not RB 1427 1429 by by IN 1430 1447 mineralocorticoid mineralocorticoid NN 1448 1456 receptor receptor NN 1456 1457 , , , 1458 1460 as as IN 1461 1466 shown show VBN 1467 1469 by by IN 1470 1480 inhibition inhibition NN 1481 1492 experiments experiment NNS 1493 1497 with with IN 1498 1506 specific specific JJ 1507 1518 antagonists antagonist NNS 1518 1519 . . . 1520 1527 Finally finally RB 1527 1528 , , , 1529 1531 it it PRP 1532 1534 is be VBZ 1535 1547 demonstrated demonstrate VBN 1548 1552 that that IN 1553 1564 macrophages macrophage NNS 1565 1567 in in IN 1568 1571 the the DT 1572 1579 adrenal adrenal JJ 1580 1585 gland gland NN 1585 1586 , , , 1587 1590 the the DT 1591 1596 major major JJ 1597 1601 site site NN 1602 1604 of of IN 1605 1615 endogenous endogenous JJ 1616 1618 GC GC NNP 1619 1629 production production NN 1629 1630 , , , 1631 1638 express express VBP 1639 1651 sialoadhesin sialoadhesin NN 1651 1652 . . . 1653 1657 This this DT 1658 1663 study study NN 1664 1676 demonstrates demonstrate VBZ 1677 1681 that that IN 1682 1684 GC GC NNP 1685 1688 act act VBP 1689 1691 as as IN 1692 1693 a a DT 1694 1701 primary primary JJ 1702 1709 inducer inducer NN 1710 1712 of of IN 1713 1725 sialoadhesin sialoadhesin NN 1726 1736 expression expression NN 1737 1739 on on IN 1740 1743 rat rat NN 1744 1755 macrophages macrophage NNS 1755 1756 , , , 1757 1760 and and CC 1761 1765 that that IN 1766 1769 the the DT 1770 1778 response response NN 1779 1782 can can MD 1783 1785 be be VB 1786 1794 enhanced enhance VBN 1795 1797 by by IN 1798 1806 IFN-beta IFN-beta NNP 1806 1807 , , , 1808 1809 T t NN 1810 1822 cell-derived cell-derived JJ 1823 1832 cytokines cytokine NNS 1832 1833 , , , 1834 1836 or or CC 1837 1840 LPS LPS NNP 1840 1841 . . .