0 2 E3 E3 NNP 2 3 , , , 4 5 a a DT 6 28 hematopoietic-specific hematopoietic-specific JJ 29 39 transcript transcript NN 40 48 directly directly RB 49 58 regulated regulate VBN 59 61 by by IN 62 65 the the DT 66 74 retinoic retinoic JJ 75 79 acid acid NN 80 88 receptor receptor NN 89 94 alpha alpha NN 94 95 . . . 97 105 Retinoic retinoic JJ 106 110 acid acid NN 111 112 ( ( ( 112 123 RA)-induced ra)-induced JJ 124 134 maturation maturation NN 135 143 mediated mediate VBN 144 146 by by IN 147 150 the the DT 151 159 retinoic retinoic JJ 160 164 acid acid NN 165 173 receptor receptor NN 174 179 alpha alpha NN 180 181 ( ( ( 181 184 RAR RAR NNP 185 190 alpha alpha NN 190 191 ) ) ) 192 195 has have VBZ 196 200 been be VBN 201 211 implicated implicate VBN 212 214 in in IN 215 222 myeloid myeloid JJ 223 234 development development NN 234 235 . . . 236 238 We we PRP 239 243 have have VBP 244 248 used use VBN 249 261 differential differential JJ 262 275 hybridization hybridization NN 276 284 analysis analysis NN 285 287 of of IN 288 289 a a DT 290 294 cDNA cdna NN 295 302 library library NN 303 314 constructed construct VBN 315 319 from from IN 320 323 the the DT 324 330 murine murine JJ 331 343 RA-inducible ra-inducible JJ 344 348 MPRO mpro NN 349 361 promyelocyte promyelocyte NN 362 366 cell cell NN 367 371 line line NN 372 374 to to TO 375 383 identify identify VB 384 399 immediate-early immediate-early JJ 400 405 genes gene NNS 406 413 induced induce VBN 414 416 by by IN 417 419 RA RA NNP 420 426 during during IN 427 439 granulocytic granulocytic JJ 440 455 differentiation differentiation NN 455 456 . . . 457 459 E3 E3 NNP 459 460 , , , 461 464 one one CD 465 467 of of IN 468 472 nine nine CD 473 482 sequences sequence NNS 483 493 identified identify VBN 493 494 , , , 495 498 was be VBD 499 510 upregulated upregulate VBN 511 513 in in IN 514 516 an an DT 517 532 immediate-early immediate-early JJ 533 539 manner manner NN 539 540 , , , 541 545 with with IN 546 556 transcript transcript NN 557 563 levels level NNS 564 571 peaking peak VBG 572 577 after after IN 578 580 60 60 CD 581 588 minutes minute NNS 589 597 exposure exposure NN 598 600 to to TO 601 603 RA RA NNP 603 604 . . . 605 607 E3 e3 NN 608 619 transcripts transcript NNS 620 624 were be VBD 625 637 RA-inducible ra-inducible JJ 638 640 in in IN 641 645 HL60 hl60 NN 646 651 cells cell NNS 651 652 , , , 653 656 but but CC 657 660 not not RB 661 663 in in IN 664 666 an an DT 667 679 RA-resistant ra-resistant JJ 680 688 subclone subclone NN 688 689 , , , 690 695 HL60R HL60R NNP 695 696 , , , 697 701 that that WDT 702 709 harbors harbor VBZ 710 711 a a DT 712 719 mutated mutate VBN 720 723 RAR rar NN 724 729 alpha alpha NN 730 734 gene gene NN 734 735 . . . 736 743 However however RB 743 744 , , , 745 749 when when WRB 750 755 HL60R HL60R NNP 756 761 cells cell NNS 762 766 were be VBD 767 777 transduced transduce VBN 778 782 with with IN 783 784 a a DT 785 795 functional functional JJ 796 800 copy copy NN 801 803 of of IN 804 807 the the DT 808 811 RAR RAR NNP 812 817 alpha alpha NN 818 822 gene gene NN 822 823 , , , 824 826 RA RA NNP 827 834 induced induce VBD 835 836 a a DT 837 844 10-fold 10-fold JJ 845 853 increase increase NN 854 856 in in IN 857 859 E3 e3 NN 860 864 mRNA mrna NN 865 871 levels level NNS 871 872 . . . 873 875 E3 e3 NN 876 887 transcripts transcript NNS 888 891 are be VBP 892 899 present present JJ 900 902 in in IN 903 906 the the DT 907 914 myeloid myeloid JJ 914 915 , , , 916 926 B-lymphoid b-lymphoid JJ 926 927 , , , 928 931 and and CC 932 941 erythroid erythroid JJ 942 950 lineages lineage NNS 950 951 , , , 952 958 absent absent JJ 959 961 in in IN 962 978 nonhematopoietic nonhematopoietic JJ 979 984 cells cell NNS 984 985 , , , 986 989 and and CC 990 996 encode encode VBP 997 998 a a DT 999 1005 highly highly RB 1006 1017 hydrophobic hydrophobic JJ 1017 1018 , , , 1019 1030 potentially potentially RB 1031 1045 phosphorylated phosphorylate VBN 1046 1057 polypeptide polypeptide NN 1058 1060 of of IN 1061 1068 unknown unknown JJ 1069 1077 function function NN 1078 1082 with with IN 1083 1094 significant significant JJ 1095 1103 homology homology NN 1104 1106 to to TO 1107 1108 a a DT 1109 1117 putative putative JJ 1118 1125 protein protein NN 1126 1135 expressed express VBN 1136 1138 in in IN 1139 1146 myeloid myeloid JJ 1147 1152 cells cell NNS 1152 1153 . . . 1154 1157 The the DT 1158 1164 murine murine JJ 1165 1167 E3 e3 NN 1168 1176 promoter promoter NN 1177 1184 harbors harbor VBZ 1185 1186 a a DT 1187 1193 single single JJ 1194 1203 bipartite bipartite JJ 1204 1212 retinoic retinoic JJ 1213 1217 acid acid NN 1218 1226 response response NN 1227 1234 element element NN 1235 1240 which which WDT 1241 1243 in in IN 1244 1253 transient transient JJ 1254 1266 transfection transfection NN 1267 1273 assays assay NNS 1274 1283 conferred confer VBD 1284 1286 RA RA NNP 1287 1298 sensitivity sensitivity NN 1298 1299 . . . 1300 1305 These these DT 1306 1313 results result NNS 1314 1322 indicate indicate VBP 1323 1327 that that IN 1328 1330 E3 e3 NN 1331 1333 is be VBZ 1334 1335 a a DT 1336 1358 hematopoietic-specific hematopoietic-specific JJ 1359 1363 gene gene NN 1364 1368 that that WDT 1369 1371 is be VBZ 1372 1374 an an DT 1375 1384 immediate immediate JJ 1385 1391 target target NN 1392 1395 for for IN 1396 1399 the the DT 1400 1409 activated activate VBN 1410 1413 RAR rar NN 1414 1419 alpha alpha NN 1420 1426 during during IN 1427 1439 myelopoiesis myelopoiesis NN 1439 1440 . . .