0 17 Selenium-mediated selenium-mediated JJ 18 28 inhibition inhibition NN 29 31 of of IN 32 45 transcription transcription NN 46 52 factor factor NN 53 61 NF-kappa NF-kappa NNP 62 63 B B NNP 64 67 and and CC 68 73 HIV-1 HIV-1 NNP 74 77 LTR LTR NNP 78 86 promoter promoter NN 87 95 activity activity NN 95 96 . . . 98 101 The the DT 102 112 eukaryotic eukaryotic JJ 113 126 transcription transcription NN 127 133 factor factor NN 134 142 NF-kappa NF-kappa NNP 143 144 B B NNP 145 147 is be VBZ 148 156 involved involve VBN 157 159 in in IN 160 163 the the DT 164 173 inducible inducible JJ 174 184 expression expression NN 185 187 of of IN 188 195 various various JJ 196 208 inflammatory inflammatory JJ 209 214 genes gene NNS 215 217 as as RB 218 222 well well RB 223 225 as as IN 226 228 in in IN 229 234 HIV-1 HIV-1 NNP 235 246 replication replication NN 246 247 . . . 248 258 Activation Activation NNP 259 261 of of IN 262 270 NF-kappa NF-kappa NNP 271 272 B B NNP 273 275 is be VBZ 276 283 induced induce VBN 284 286 by by IN 287 298 prooxidants prooxidant NNS 299 302 and and CC 303 310 several several JJ 311 318 stimuli stimulus NNS 319 328 eliciting elicit VBG 329 338 oxidative oxidative JJ 339 345 stress stress NN 345 346 , , , 347 351 such such JJ 352 354 as as IN 355 364 cytokines cytokine NNS 364 365 , , , 366 384 lipopolysaccharide lipopolysaccharide NN 384 385 , , , 386 388 UV UV NNP 389 400 irradiation irradiation NN 401 404 and and CC 405 410 other other JJ 411 420 mediators mediator NNS 420 421 . . . 422 429 Various various JJ 430 442 antioxidants antioxidant NNS 443 450 inhibit inhibit VBP 451 459 NF-kappa NF-kappa NNP 460 461 B B NNP 462 472 activation activation NN 473 475 in in IN 476 484 response response NN 485 487 to to TO 488 493 these these DT 494 501 stimuli stimulus NNS 501 502 . . . 503 505 In in IN 506 510 this this DT 511 516 study study NN 516 517 , , , 518 520 we we PRP 521 525 have have VBP 526 538 investigated investigate VBN 539 542 the the DT 543 550 effects effect NNS 551 553 of of IN 554 562 selenium selenium NN 562 563 , , , 564 566 an an DT 567 575 integral integral JJ 576 585 component component NN 586 588 of of IN 589 600 glutathione glutathione NN 601 611 peroxidase peroxidase NN 612 613 ( ( ( 613 616 GPX GPX NNP 616 617 ) ) ) 617 618 , , , 619 621 on on IN 622 630 NF-kappa NF-kappa NNP 631 632 B B NNP 633 643 activation activation NN 643 644 . . . 645 647 In in IN 648 665 selenium-deprived selenium-deprived JJ 666 672 Jurkat Jurkat NNP 673 676 and and CC 677 682 ESb-L ESb-L NNP 683 684 T t NN 685 696 lymphocytes lymphocyte NNS 696 697 , , , 698 713 supplementation supplementation NN 714 716 of of IN 717 725 selenium selenium NN 726 729 led lead VBD 730 732 to to TO 733 734 a a DT 735 746 substantial substantial JJ 747 755 increase increase NN 756 758 of of IN 759 762 GPX GPX NNP 763 771 activity activity NN 771 772 . . . 773 781 Analysis Analysis NNP 782 784 of of IN 785 788 DNA DNA NNP 789 796 binding binding NN 797 805 revealed reveal VBD 806 810 that that IN 811 819 NF-kappa NF-kappa NNP 820 821 B B NNP 822 832 activation activation NN 833 835 in in IN 836 844 response response NN 845 847 to to TO 848 851 TNF TNF NNP 852 855 was be VBD 856 869 significantly significantly RB 870 879 inhibited inhibit VBN 880 885 under under IN 886 891 these these DT 892 902 conditions condition NNS 902 903 . . . 904 912 Likewise likewise RB 912 913 , , , 914 922 reporter reporter NN 923 927 gene gene NN 928 934 assays assay NNS 935 940 using use VBG 941 951 luciferase luciferase NN 952 962 constructs construct NNS 963 969 driven drive VBN 970 972 by by IN 973 976 the the DT 977 982 HIV-1 HIV-1 NNP 983 987 long long JJ 988 996 terminal terminal JJ 997 1003 repeat repeat NN 1004 1010 showed show VBD 1011 1012 a a DT 1013 1027 dose-dependent dose-dependent JJ 1028 1038 inhibition inhibition NN 1039 1041 of of IN 1042 1050 NF-kappa NF-kappa NNP 1051 1052 B B NNP 1053 1063 controlled controlled JJ 1064 1068 gene gene NN 1069 1079 expression expression NN 1080 1082 by by IN 1083 1091 selenium selenium NN 1091 1092 . . . 1093 1096 The the DT 1097 1104 effects effect NNS 1105 1107 of of IN 1108 1116 selenium selenium NN 1117 1121 were be VBD 1122 1130 specific specific JJ 1131 1134 for for IN 1135 1143 NF-kappa NF-kappa NNP 1144 1145 B B NNP 1145 1146 , , , 1147 1152 since since IN 1153 1156 the the DT 1157 1165 activity activity NN 1166 1168 of of IN 1169 1172 the the DT 1173 1186 transcription transcription NN 1187 1193 factor factor NN 1194 1198 AP-1 ap-1 NN 1199 1202 was be VBD 1203 1206 not not RB 1207 1217 suppressed suppress VBN 1217 1218 . . . 1219 1224 These these DT 1225 1229 data datum NNS 1230 1237 suggest suggest VBP 1238 1242 that that IN 1243 1251 selenium selenium NN 1252 1267 supplementation supplementation NN 1268 1271 may may MD 1272 1274 be be VB 1275 1279 used use VBN 1280 1282 to to TO 1283 1291 modulate modulate VB 1292 1295 the the DT 1296 1306 expression expression NN 1307 1309 of of IN 1310 1318 NF-kappa NF-kappa NNP 1319 1320 B B NNP 1321 1327 target target NN 1328 1333 genes gene NNS 1334 1337 and and CC 1338 1343 HIV-1 HIV-1 NNP 1343 1344 . . .