0 5 Human human JJ 6 11 TAFII TAFII NNP 12 15 105 105 CD 16 18 is be VBZ 19 20 a a DT 21 25 cell cell NN 26 39 type-specific type-specific JJ 40 45 TFIID TFIID NNP 46 53 subunit subunit NN 54 61 related relate VBN 62 64 to to TO 65 74 hTAFII130 htafii130 NN 74 75 . . . 77 79 We we PRP 80 90 previously previously RB 91 104 characterized characterize VBD 105 115 Drosophila Drosophila NNP 116 119 and and CC 120 125 human human JJ 126 129 TAF TAF NNP 130 138 subunits subunit NNS 139 143 that that WDT 144 148 make make VBP 149 151 up up RP 152 155 the the DT 156 160 core core NN 161 166 TFIID tfiid NN 167 174 complex complex NN 175 180 found find VBN 181 183 in in IN 184 187 all all DT 188 193 cells cell NNS 193 194 . . . 195 199 Here here RB 199 200 , , , 201 203 we we PRP 204 210 report report VBP 211 215 that that IN 216 230 differentiated differentiate VBN 231 232 B B NNP 233 238 cells cell NNS 239 246 contain contain VBP 247 248 a a DT 249 254 novel novel JJ 255 272 substoichiometric substoichiometric JJ 273 276 TAF taf NN 277 279 of of IN 280 283 105 105 CD 284 287 kDa kda NN 288 291 not not RB 292 297 found find VBN 298 308 associated associate VBN 309 313 with with IN 314 319 TFIID TFIID NNP 320 328 isolated isolate VBN 329 333 from from IN 334 339 other other JJ 340 344 cell cell NN 345 350 types type NNS 350 351 . . . 352 355 The the DT 356 360 cDNA cdna NN 361 369 encoding encoding NN 370 379 hTAFII105 htafii105 NN 380 387 reveals reveal VBZ 388 389 a a DT 390 396 highly highly RB 397 406 conserved conserve VBN 407 417 C-terminal c-terminal JJ 418 424 domain domain NN 425 431 shared share VBN 432 434 by by IN 435 444 hTAFII130 htafii130 NN 445 448 and and CC 449 458 oTAFII110 otafii110 NN 458 459 , , , 460 465 while while IN 466 469 the the DT 470 480 N-terminal n-terminal JJ 481 492 coactivator coactivator NN 493 499 domain domain NN 500 503 has have VBZ 504 512 diverged diverge VBN 513 526 significantly significantly RB 526 527 . . . 528 531 All all DT 532 537 cells cell NNS 538 544 tested test VBN 545 552 express express VBP 553 561 TAFII105 tafii105 NN 562 566 mRNA mRNA NNP 566 567 , , , 568 571 but but CC 572 576 only only RB 577 578 B B NNP 579 584 cells cell NNS 585 592 contain contain VBP 593 604 significant significant JJ 605 611 levels level NNS 612 614 of of IN 615 622 protein protein NN 623 633 associated associate VBN 634 638 with with IN 639 644 TFIID TFIID NNP 644 645 . . . 646 655 Transient transient JJ 656 670 overexpression overexpression NN 671 673 of of IN 674 683 hTAFII105 htafii105 NN 684 695 selectively selectively RB 696 705 squelches squelch VBZ 706 709 the the DT 710 723 transcription transcription NN 724 726 of of IN 727 731 some some DT 732 737 genes gene NNS 738 740 in in IN 741 742 B B NNP 743 748 cells cell NNS 748 749 . . . 750 755 These these DT 756 766 properties property NNS 767 774 suggest suggest VBP 775 779 that that IN 780 788 TAFII105 tafii105 NN 789 791 is be VBZ 792 793 a a DT 794 798 cell cell NN 799 812 type-specific type-specific JJ 813 820 subunit subunit NN 821 823 of of IN 824 829 TFIID TFIID NNP 830 834 that that WDT 835 838 may may MD 839 841 be be VB 842 853 responsible responsible JJ 854 857 for for IN 858 867 mediating mediate VBG 868 881 transcription transcription NN 882 884 by by IN 885 886 a a DT 887 893 subset subset NN 894 896 of of IN 897 907 activators activator NNS 908 910 in in IN 911 912 B B NNP 913 918 cells cell NNS 918 919 . . .