0 13 IL-12-induced il-12-induced JJ 14 24 activation activation NN 25 27 of of IN 28 30 NK NK NNP 31 34 and and CC 35 36 T t NN 37 42 cells cell NNS 43 49 occurs occur VBZ 50 52 in in IN 53 56 the the DT 57 64 absence absence NN 65 67 of of IN 68 83 immediate-early immediate-early JJ 84 94 activation activation NN 95 99 gene gene NN 100 110 expression expression NN 110 111 . . . 113 116 The the DT 117 126 responses response NNS 127 129 of of IN 130 141 lymphocytes lymphocyte NNS 142 144 to to TO 145 149 IL-2 il-2 NN 150 153 and and CC 154 159 IL-12 il-12 NN 159 160 , , , 161 170 involving involve VBG 171 184 proliferation proliferation NN 184 185 , , , 186 201 differentiation differentiation NN 201 202 , , , 203 206 and and CC 207 215 cytokine cytokine NN 216 226 production production NN 226 227 , , , 228 231 are be VBP 232 236 only only RB 237 246 partially partially RB 247 258 overlapping overlap VBG 258 259 , , , 260 263 and and CC 264 267 may may MD 268 274 depend depend VB 275 277 on on IN 278 285 induced induce VBN 286 298 differential differential JJ 299 309 expression expression NN 310 312 of of IN 313 321 specific specific JJ 322 326 sets set NNS 327 329 of of IN 330 335 genes gene NNS 335 336 . . . 337 342 Using use VBG 343 364 reverse-transcription reverse-transcription NN 365 368 PCR PCR NNP 369 381 differential differential JJ 382 389 display display NN 389 390 , , , 391 393 we we PRP 394 402 isolated isolate VBD 403 405 an an DT 406 410 mRNA mrna NN 411 418 species specie NNS 419 428 expressed express VBN 429 431 in in IN 432 437 IL-2- IL-2- NNP 438 441 but but CC 442 445 not not RB 446 462 IL-12-stimulated il-12-stimulated JJ 463 465 NK nk NN 466 471 cells cell NNS 471 472 . . . 473 477 This this DT 478 481 was be VBD 482 492 identified identify VBN 493 495 as as IN 496 499 the the DT 500 504 mRNA mrna NN 505 513 encoding encode VBG 514 517 the the DT 518 531 transcription transcription NN 532 538 factor factor NN 539 544 egr-1 egr-1 NN 544 545 , , , 546 551 which which WDT 552 554 is be VBZ 555 564 expressed express VBN 565 569 with with IN 570 574 fast fast JJ 575 583 kinetics kinetic NNS 584 586 in in IN 587 588 T t NN 589 592 and and CC 593 595 NK nk NN 596 601 cells cell NNS 602 606 upon upon IN 607 611 IL-2 il-2 NN 611 612 , , , 613 616 but but CC 617 620 not not RB 621 626 IL-12 il-12 NN 626 627 , , , 628 639 stimulation stimulation NN 639 640 . . . 641 649 Analysis Analysis NNP 650 652 of of IN 653 656 the the DT 657 669 accumulation accumulation NN 670 672 of of IN 673 686 mRNA-encoding mrna-encoding JJ 687 694 members member NNS 695 697 of of IN 698 701 the the DT 702 706 AP-1 ap-1 NN 707 720 transcription transcription NN 721 727 factor factor NN 728 734 family family NN 735 747 demonstrated demonstrate VBD 748 752 that that IN 753 758 c-fos c-fos NN 759 762 and and CC 763 767 junB junb NN 768 771 are be VBP 772 776 also also RB 777 786 expressed express VBN 787 791 upon upon IN 792 803 stimulation stimulation NN 804 806 of of IN 807 809 NK NK NNP 810 813 and and CC 814 815 T t NN 816 821 cells cell NNS 822 826 with with IN 827 831 IL-2 il-2 NN 831 832 , , , 833 836 but but CC 837 840 not not RB 841 846 IL-12 il-12 NN 846 847 , , , 848 855 whereas whereas IN 856 866 expression expression NN 867 869 of of IN 870 875 c-jun c-jun NN 876 879 and and CC 880 884 junD jund NN 885 887 is be VBZ 888 891 not not RB 892 900 modified modify VBN 901 903 by by IN 904 910 either either DT 911 919 cytokine cytokine NN 919 920 . . . 921 932 Accordingly accordingly RB 932 933 , , , 934 943 increased increase VBN 944 948 AP-1 ap-1 NN 949 960 DNA-binding dna-binding JJ 961 969 activity activity NN 970 973 and and CC 974 988 AP-1-dependent ap-1-dependent JJ 989 1004 transcriptional transcriptional JJ 1005 1013 activity activity NN 1014 1018 were be VBD 1019 1027 detected detect VBN 1028 1039 exclusively exclusively RB 1040 1042 in in IN 1043 1058 IL-2-stimulated il-2-stimulated JJ 1059 1064 cells cell NNS 1064 1065 . . . 1066 1074 Analysis Analysis NNP 1075 1077 of of IN 1078 1081 the the DT 1082 1092 expression expression NN 1093 1095 of of IN 1096 1101 genes gene NNS 1102 1110 reported report VBN 1111 1113 to to TO 1114 1122 regulate regulate VB 1123 1139 cytokine-induced cytokine-induced JJ 1140 1153 proliferation proliferation NN 1154 1166 demonstrated demonstrate VBD 1167 1171 that that IN 1172 1176 both both CC 1177 1181 IL-2 il-2 NN 1182 1185 and and CC 1186 1191 IL-12 il-12 NN 1192 1198 induce induce VBP 1199 1204 c-myc c-myc NN 1205 1209 mRNA mrna NN 1210 1222 accumulation accumulation NN 1223 1225 in in IN 1226 1228 NK NK NNP 1229 1232 and and CC 1233 1234 T t NN 1235 1240 cells cell NNS 1240 1241 , , , 1242 1249 whereas whereas IN 1250 1254 only only RB 1255 1259 IL-2 il-2 NN 1260 1267 induces induce VBZ 1268 1273 bcl-2 bcl-2 NN 1274 1284 expression expression NN 1284 1285 . . . 1286 1289 Our our PRP$ 1290 1294 data datum NNS 1295 1302 provide provide VBP 1303 1306 the the DT 1307 1312 first first JJ 1313 1326 demonstration demonstration NN 1327 1331 that that IN 1332 1346 IL-12-mediated il-12-mediated JJ 1347 1357 activation activation NN 1358 1360 of of IN 1361 1362 T t NN 1363 1366 and and CC 1367 1369 NK nk NN 1370 1375 cells cell NNS 1376 1380 does do VBZ 1381 1384 not not RB 1385 1392 involve involve VB 1393 1403 expression expression NN 1404 1406 of of IN 1407 1414 members member NNS 1415 1417 of of IN 1418 1421 the the DT 1422 1437 immediate-early immediate-early JJ 1438 1448 activation activation NN 1449 1454 genes gene NNS 1455 1461 family family NN 1462 1463 ( ( ( 1463 1468 egr-1 egr-1 NN 1468 1469 , , , 1470 1475 c-fos c-fos NN 1475 1476 , , , 1477 1480 and and CC 1481 1485 junB junB NNP 1485 1486 ) ) ) 1486 1487 , , , 1488 1492 AP-1 ap-1 NN 1493 1508 transcriptional transcriptional JJ 1509 1517 activity activity NN 1517 1518 , , , 1519 1521 or or CC 1522 1527 bcl-2 bcl-2 NN 1528 1538 expression expression NN 1538 1539 . . . 1540 1544 This this DT 1545 1554 indicates indicate VBZ 1555 1559 that that IN 1560 1570 functional functional JJ 1571 1582 differences difference NNS 1583 1591 observed observe VBN 1592 1594 in in IN 1595 1600 IL-2- il-2- NN 1601 1604 and and CC 1605 1621 IL-12-stimulated il-12-stimulated JJ 1622 1627 cells cell NNS 1628 1631 may may MD 1632 1638 depend depend VB 1638 1639 , , , 1640 1642 at at IN 1643 1648 least least JJS 1649 1651 in in IN 1652 1656 part part NN 1656 1657 , , , 1658 1660 on on IN 1661 1673 differential differential JJ 1674 1678 gene gene NN 1679 1689 regulation regulation NN 1689 1690 . . .