0	7	Octamer	Octamer	NNP
8	19	independent	independent	JJ
20	30	activation	activation	NN
31	33	of	of	IN
34	47	transcription	transcription	NN
48	52	from	from	IN
53	56	the	the	DT
57	62	kappa	kappa	NN
63	77	immunoglobulin	immunoglobulin	NN
78	86	germline	germline	NN
87	95	promoter	promoter	NN
95	96	.	.	.

98	106	Previous	previous	JJ
107	115	analyses	analysis	NNS
116	118	of	of	IN
119	133	immunoglobulin	immunoglobulin	NN
134	135	V	v	JJ
136	142	region	region	NN
143	152	promoters	promoter	NNS
153	156	has	have	VBZ
157	160	led	lead	VBN
161	163	to	to	TO
164	167	the	the	DT
168	177	discovery	discovery	NN
178	180	of	of	IN
181	182	a	a	DT
183	189	common	common	JJ
190	197	octamer	octamer	NN
198	203	motif	motif	NN
204	209	which	which	WDT
210	212	is	be	VBZ
213	225	functionally	functionally	RB
226	235	important	important	JJ
236	238	in	in	IN
239	242	the	the	DT
243	258	tissue-specific	tissue-specific	JJ
259	262	and	and	CC
263	278	developmentally	developmentally	RB
279	288	regulated	regulate	VBN
289	304	transcriptional	transcriptional	JJ
305	315	activation	activation	NN
316	318	of	of	IN
319	333	immunoglobulin	immunoglobulin	NN
334	339	genes	gene	NNS
339	340	.	.	.

341	344	The	the	DT
345	353	germline	germline	NN
354	363	promoters	promoter	NNS
364	365	(	(	(
365	367	Ko	Ko	NNP
367	368	)	)	)
369	376	located	located	JJ
377	385	upstream	upstream	RB
386	388	of	of	IN
389	392	the	the	DT
393	394	J	J	NNP
395	401	region	region	NN
402	406	gene	gene	NN
407	415	segments	segment	NNS
416	418	of	of	IN
419	422	the	the	DT
423	428	kappa	kappa	NN
429	434	locus	locus	NN
435	439	also	also	RB
440	447	contain	contain	VBP
448	450	an	an	DT
451	458	octamer	octamer	NN
459	464	motif	motif	NN
465	466	(	(	(
466	476	containing	contain	VBG
477	478	a	a	DT
479	485	single	single	JJ
486	490	base	base	NN
491	495	pair	pair	NN
496	504	mutation	mutation	NN
505	508	and	and	CC
509	517	referred	refer	VBN
518	520	to	to	TO
521	523	as	as	IN
524	527	the	the	DT
528	535	variant	variant	JJ
536	543	octamer	octamer	NN
543	544	)	)	)
545	550	which	which	WDT
551	554	has	have	VBZ
555	559	been	be	VBN
560	565	shown	show	VBN
566	576	previously	previously	RB
577	579	to	to	TO
580	584	bind	bind	VB
585	590	Oct-1	oct-1	NN
591	594	and	and	CC
595	600	Oct-2	oct-2	NN
601	614	transcription	transcription	NN
615	622	factors	factor	NNS
623	625	in	in	FW
626	631	vitro	vitro	FW
631	632	.	.	.

633	635	To	to	TO
636	643	further	further	RB
644	653	elucidate	elucidate	VB
654	657	the	the	DT
658	662	role	role	NN
663	665	of	of	IN
666	670	this	this	DT
671	678	variant	variant	JJ
679	686	octamer	octamer	NN
687	692	motif	motif	NN
693	695	in	in	IN
696	699	the	the	DT
700	710	regulation	regulation	NN
711	713	of	of	IN
714	722	germline	germline	NN
723	736	transcription	transcription	NN
737	741	from	from	IN
742	745	the	the	DT
746	758	unrearranged	unrearranged	JJ
759	764	kappa	kappa	NN
765	770	locus	locus	NN
770	771	,	,	,
772	774	we	we	PRP
775	779	have	have	VBP
780	791	quantitated	quantitate	VBN
792	795	the	the	DT
796	804	relative	relative	JJ
805	812	binding	binding	NN
813	821	affinity	affinity	NN
822	824	of	of	IN
825	830	Oct-1	Oct-1	NNP
831	834	and	and	CC
835	840	Oct-2	oct-2	NN
841	844	for	for	IN
845	848	the	the	DT
849	856	variant	variant	JJ
857	864	octamer	octamer	NN
865	870	motif	motif	NN
871	874	and	and	CC
875	885	determined	determine	VBD
886	889	the	the	DT
890	900	functional	functional	JJ
901	905	role	role	NN
906	908	of	of	IN
909	913	this	this	DT
914	921	octamer	octamer	NN
922	927	motif	motif	NN
928	930	in	in	IN
931	946	transcriptional	transcriptional	JJ
947	957	activation	activation	NN
957	958	.	.	.

959	961	We	we	PRP
962	966	find	find	VBP
967	971	that	that	IN
971	972	,	,	,
973	981	although	although	IN
982	985	the	the	DT
986	993	variant	variant	JJ
994	1001	octamer	octamer	NN
1002	1007	motif	motif	NN
1008	1013	binds	bind	VBZ
1014	1019	Oct-1	oct-1	NN
1020	1023	and	and	CC
1024	1029	Oct-2	oct-2	NN
1030	1032	in	in	FW
1033	1038	vitro	vitro	FW
1039	1043	with	with	IN
1044	1050	5-fold	5-fold	RB
1051	1056	lower	low	JJR
1057	1065	affinity	affinity	NN
1066	1070	than	than	IN
1071	1074	the	the	DT
1075	1084	consensus	consensus	NN
1085	1092	octamer	octamer	NN
1093	1098	motif	motif	NN
1098	1099	,	,	,
1100	1108	mutation	mutation	NN
1109	1111	of	of	IN
1112	1115	the	the	DT
1116	1123	variant	variant	JJ
1124	1131	octamer	octamer	NN
1132	1137	motif	motif	NN
1138	1140	to	to	TO
1141	1147	either	either	CC
1148	1149	a	a	DT
1150	1159	consensus	consensus	NN
1160	1167	octamer	octamer	NN
1168	1170	or	or	CC
1171	1182	non-octamer	non-octamer	JJ
1183	1188	motif	motif	NN
1189	1192	has	have	VBZ
1193	1195	no	no	DT
1196	1202	effect	effect	NN
1203	1205	on	on	IN
1206	1221	transcriptional	transcriptional	JJ
1222	1232	activation	activation	NN
1233	1237	from	from	IN
1238	1241	the	the	DT
1242	1250	germline	germline	NN
1251	1259	promoter	promoter	NN
1259	1260	.	.	.

1261	1263	We	we	PRP
1264	1268	also	also	RB
1269	1273	find	find	VBP
1274	1285	significant	significant	JJ
1286	1297	differences	difference	NNS
1298	1300	in	in	IN
1301	1311	activation	activation	NN
1312	1314	of	of	IN
1315	1323	germline	germline	NN
1324	1327	and	and	CC
1328	1329	V	v	JJ
1330	1336	region	region	NN
1337	1346	promoters	promoter	NNS
1347	1349	by	by	IN
1350	1355	kappa	kappa	NN
1356	1365	enhancers	enhancer	NNS
1365	1366	.	.	.

1367	1370	Our	our	PRP$
1371	1378	results	result	NNS
1379	1386	suggest	suggest	VBP
1387	1391	that	that	IN
1392	1395	the	the	DT
1396	1404	germline	germline	NN
1405	1414	promoters	promoter	NNS
1415	1418	and	and	CC
1419	1420	V	v	JJ
1421	1427	region	region	NN
1428	1437	promoters	promoter	NNS
1438	1444	differ	differ	VBP
1445	1447	in	in	IN
1448	1453	their	their	PRP$
1454	1464	dependence	dependence	NN
1465	1467	on	on	IN
1468	1475	octamer	octamer	NN
1476	1479	for	for	IN
1480	1490	activation	activation	NN
1491	1494	and	and	CC
1495	1502	respond	respond	VBP
1503	1514	differently	differently	RB
1515	1517	to	to	TO
1518	1526	enhancer	enhancer	NN
1527	1537	activation	activation	NN
1537	1538	.	.	.

1539	1544	These	these	DT
1545	1553	findings	finding	NNS
1554	1558	have	have	VBP
1559	1568	important	important	JJ
1569	1581	implications	implication	NNS
1582	1584	in	in	IN
1585	1595	regulation	regulation	NN
1596	1598	of	of	IN
1599	1607	germline	germline	NN
1608	1621	transcription	transcription	NN
1622	1624	as	as	RB
1625	1629	well	well	RB
1630	1632	as	as	IN
1633	1644	concomitant	concomitant	JJ
1645	1655	activation	activation	NN
1656	1658	of	of	IN
1659	1662	the	the	DT
1663	1666	V-J	v-j	JJ
1667	1680	recombination	recombination	NN
1681	1683	of	of	IN
1684	1687	the	the	DT
1688	1693	kappa	kappa	NN
1694	1699	light	light	JJ
1700	1705	chain	chain	NN
1706	1711	locus	locus	NN
1711	1712	.	.	.