0 11 Interaction interaction NN 12 14 of of IN 15 28 transcription transcription NN 29 36 factors factor NNS 37 41 RFX1 rfx1 NN 42 45 and and CC 46 51 MIBP1 mibp1 NN 52 56 with with IN 57 60 the the DT 61 66 gamma gamma NN 67 72 motif motif NN 73 75 of of IN 76 79 the the DT 80 88 negative negative JJ 89 99 regulatory regulatory JJ 100 107 element element NN 108 110 of of IN 111 114 the the DT 115 124 hepatitis hepatitis NNP 125 126 B B NNP 127 132 virus virus NN 133 137 core core NN 138 146 promoter promoter NN 146 147 . . . 149 152 The the DT 153 161 negative negative JJ 162 172 regulatory regulatory JJ 173 180 element element NN 181 182 ( ( ( 182 185 NRE NRE NNP 185 186 ) ) ) 187 189 of of IN 190 193 the the DT 194 203 hepatitis hepatitis NNP 204 205 B B NNP 206 211 virus virus NN 212 213 ( ( ( 213 216 HBV HBV NNP 216 217 ) ) ) 218 222 core core NN 223 231 promoter promoter NN 232 240 contains contain VBZ 241 246 three three CD 247 257 subregions subregion NNS 258 263 which which WDT 264 267 act act VBP 268 283 synergistically synergistically RB 284 286 to to TO 287 295 suppress suppress VB 296 300 core core NN 301 309 promoter promoter NN 310 318 activity activity NN 318 319 . . . 320 323 One one CD 324 326 of of IN 327 332 these these DT 333 343 subregions subregion NNS 343 344 , , , 345 348 NRE NRE NNP 349 354 gamma gamma NN 354 355 , , , 356 358 is be VBZ 359 365 active active JJ 366 368 in in IN 369 373 both both CC 374 378 HeLa HeLa NNP 379 387 cervical cervical JJ 388 397 carcinoma carcinoma NN 398 403 cells cell NNS 404 407 and and CC 408 412 Huh7 huh7 NN 413 421 hepatoma hepatoma NN 422 427 cells cell NNS 428 431 and and CC 432 435 was be VBD 436 441 found find VBN 442 444 to to TO 445 447 be be VB 448 453 bound bind VBN 454 456 by by IN 457 458 a a DT 459 466 protein protein NN 467 473 factor factor NN 474 481 present present JJ 482 484 in in IN 485 489 both both DT 490 494 cell cell NN 495 500 types type NNS 500 501 . . . 502 506 Here here RB 507 509 we we PRP 510 514 show show VBP 515 519 that that IN 520 523 the the DT 524 537 transcription transcription NN 538 544 factor factor NN 545 549 RFX1 rfx1 NN 550 553 can can MD 554 558 bind bind VB 559 561 to to TO 562 565 NRE nre NN 566 571 gamma gamma NN 572 575 and and CC 576 589 transactivate transactivate VB 590 593 the the DT 594 598 core core NN 599 607 promoter promoter NN 608 615 through through IN 616 620 this this DT 621 625 site site NN 625 626 . . . 627 636 Mutations mutation NNS 637 642 which which WDT 643 652 abrogated abrogate VBD 653 656 the the DT 657 673 gene-suppressive gene-suppressive JJ 674 682 activity activity NN 683 685 of of IN 686 689 NRE NRE NNP 690 695 gamma gamma NN 696 705 prevented prevent VBD 706 710 RFX1 rfx1 NN 711 715 from from IN 716 723 binding bind VBG 724 726 to to TO 727 730 NRE nre NN 731 736 gamma gamma NN 736 737 . . . 738 740 In in IN 741 749 addition addition NN 749 750 , , , 751 755 RFX1 RFX1 NNP 756 759 can can MD 760 764 bind bind VB 765 779 simultaneously simultaneously RB 779 780 , , , 781 785 most most RBS 786 792 likely likely RB 793 795 as as IN 796 797 a a DT 798 809 heterodimer heterodimer NN 809 810 , , , 811 815 with with IN 816 819 the the DT 820 833 transcription transcription NN 834 840 factor factor NN 841 846 MIBP1 mibp1 NN 847 849 to to TO 850 853 NRE nre NN 854 859 gamma gamma NN 859 860 . . . 861 863 In in IN 864 867 the the DT 868 875 absence absence NN 876 878 of of IN 879 880 a a DT 881 887 cloned clone VBN 888 893 MIBP1 mibp1 NN 894 898 gene gene NN 899 902 for for IN 903 910 further further JJ 911 918 studies study NNS 918 919 , , , 920 922 we we PRP 923 934 hypothesize hypothesize VBP 935 939 that that IN 940 944 RFX1 rfx1 NN 945 949 acts act VBZ 950 954 with with IN 955 960 MIBP1 mibp1 NN 961 963 to to TO 964 974 negatively negatively RB 975 983 regulate regulate VB 984 987 the the DT 988 992 core core NN 993 1001 promoter promoter NN 1002 1010 activity activity NN 1011 1018 through through IN 1019 1022 the the DT 1023 1026 NRE NRE NNP 1027 1032 gamma gamma NN 1033 1037 site site NN 1037 1038 . . . 1039 1042 The the DT 1043 1050 ability ability NN 1051 1053 of of IN 1054 1058 RFX1 rfx1 NN 1059 1061 to to TO 1062 1075 transactivate transactivate VB 1076 1079 the the DT 1080 1084 core core NN 1085 1093 promoter promoter NN 1094 1100 raises raise VBZ 1101 1104 the the DT 1105 1116 possibility possibility NN 1117 1121 that that IN 1122 1126 RFX1 RFX1 NNP 1127 1130 may may MD 1131 1135 play play VB 1136 1137 a a DT 1138 1142 dual dual JJ 1143 1147 role role NN 1148 1150 in in IN 1151 1161 regulating regulate VBG 1162 1165 HBV HBV NNP 1166 1170 gene gene NN 1171 1181 expression expression NN 1181 1182 . . .