0 25 Differentiation-dependent differentiation-dependent JJ 26 36 expression expression NN 37 39 of of IN 40 41 a a DT 42 47 human human JJ 48 64 carboxylesterase carboxylesterase NN 65 67 in in IN 68 77 monocytic monocytic JJ 78 83 cells cell NNS 84 87 and and CC 88 101 transcription transcription NN 102 108 factor factor NN 109 116 binding bind VBG 117 119 to to TO 120 123 the the DT 124 132 promoter promoter NN 132 133 . . . 135 152 Carboxylesterases carboxylesteras NNS 153 157 play play VBP 158 160 an an DT 161 170 important important JJ 171 175 role role NN 176 178 in in IN 179 186 defense defense NN 187 190 and and CC 191 200 clearance clearance NN 201 211 mechanisms mechanism NNS 212 214 of of IN 215 218 the the DT 219 238 monocyte/macrophage monocyte/macrophage NN 239 245 system system NN 245 246 . . . 247 253 During during IN 254 257 the the DT 258 273 differentiation differentiation NN 274 281 process process NN 282 284 of of IN 285 290 cells cell NNS 291 295 from from IN 296 299 the the DT 300 309 monocytic monocytic JJ 310 314 cell cell NN 315 319 line line NN 320 325 THP-1 thp-1 NN 326 328 we we PRP 329 337 observed observe VBD 338 339 a a DT 340 349 transient transient JJ 350 365 transcriptional transcriptional JJ 366 378 upregulation upregulation NN 379 381 of of IN 382 383 a a DT 384 389 human human JJ 390 406 carboxylesterase carboxylesterase NN 407 415 analyzed analyze VBN 416 418 by by IN 419 424 means mean NNS 425 427 of of IN 428 436 Northern northern NN 437 442 blots blot NNS 442 443 . . . 444 446 In in IN 447 458 PMA-treated pma-treated JJ 459 464 THP-1 thp-1 NN 465 470 cells cell NNS 471 473 we we PRP 474 479 could could MD 480 486 detect detect VB 487 492 three three CD 493 498 major major JJ 499 512 transcription transcription NN 513 523 initiation initiation NN 524 529 sites site NNS 530 532 as as IN 533 541 revealed reveal VBN 542 544 by by IN 545 553 Nuclease Nuclease NNP 554 564 Protection Protection NNP 565 570 Assay Assay NNP 571 578 carried carry VBD 579 582 out out RP 583 587 with with IN 588 591 two two CD 592 603 overlapping overlap VBG 604 613 antisense antisense JJ 614 617 RNA RNA NNP 618 624 probes probe NNS 624 625 . . . 626 628 We we PRP 629 633 have have VBP 634 642 recently recently RB 643 649 cloned clone VBN 650 653 the the DT 654 670 carboxylesterase carboxylesterase NN 671 679 upstream upstream JJ 680 688 sequence sequence NN 689 692 and and CC 693 699 showed show VBD 700 703 its its PRP$ 704 709 basal basal JJ 710 718 promoter promoter NN 719 727 activity activity NN 728 730 in in IN 731 734 CHO CHO NNP 735 740 cells cell NNS 740 741 . . . 742 747 Using use VBG 748 763 electrophoretic electrophoretic JJ 764 772 mobility mobility NN 773 778 shift shift NN 779 787 analysis analysis NN 788 790 we we PRP 791 803 demonstrated demonstrate VBD 804 808 that that IN 809 812 the the DT 813 821 promoter promoter NN 822 828 region region NN 829 837 spanning span VBG 838 842 base base NN 843 848 pairs pair NNS 849 851 -1 -1 CD 852 854 to to TO 855 859 -275 -275 CD 859 860 , , , 861 866 which which WDT 867 875 contains contain VBZ 876 883 several several JJ 884 892 putative putative JJ 893 900 binding bind VBG 901 906 sites site NNS 907 910 for for IN 911 924 transcription transcription NN 925 932 factors factor NNS 932 933 , , , 934 936 is be VBZ 937 942 bound bind VBN 943 945 by by IN 946 953 nuclear nuclear JJ 954 961 factors factor NNS 962 965 Sp1 Sp1 NNP 966 969 and and CC 970 974 IRBP IRBP NNP 975 978 but but CC 979 982 not not RB 983 985 by by IN 986 992 C/EBPs C/EBP NNP 992 993 . . . 994 999 Taken take VBN 1000 1008 together together RB 1009 1014 these these DT 1015 1019 data datum NNS 1020 1028 indicate indicate VBP 1029 1033 that that IN 1034 1050 carboxylesterase carboxylesterase NN 1051 1055 gene gene NN 1056 1069 transcription transcription NN 1070 1072 in in IN 1073 1078 THP-1 thp-1 NN 1079 1084 cells cell NNS 1085 1091 starts start VBZ 1092 1094 at at IN 1095 1103 multiple multiple JJ 1104 1114 initiation initiation NN 1115 1120 sites site NNS 1121 1124 and and CC 1125 1129 that that IN 1130 1133 Sp1 Sp1 NNP 1134 1137 and and CC 1138 1142 IRBP IRBP NNP 1143 1146 may may MD 1147 1149 be be VB 1150 1158 critical critical JJ 1159 1166 factors factor NNS 1167 1170 for for IN 1171 1181 modulating modulate VBG 1182 1185 the the DT 1186 1211 differentiation-dependent differentiation-dependent JJ 1212 1225 transcription transcription NN 1226 1228 of of IN 1229 1233 this this DT 1234 1239 human human JJ 1240 1256 carboxylesterase carboxylesterase NN 1257 1261 gene gene NN 1261 1262 . . .