0 16 Characterization characterization NN 17 19 of of IN 20 23 the the DT 24 29 human human JJ 30 50 platelet/endothelial platelet/endothelial JJ 51 55 cell cell NN 56 64 adhesion adhesion NN 65 75 molecule-1 molecule-1 NN 76 84 promoter promoter NN 84 85 : : : 86 100 identification identification NN 101 103 of of IN 104 105 a a DT 106 112 GATA-2 gata-2 NN 113 120 binding binding NN 121 128 element element NN 129 137 required require VBN 138 141 for for IN 142 149 optimal optimal JJ 150 165 transcriptional transcriptional JJ 166 174 activity activity NN 174 175 . . . 177 197 Platelet/endothelial platelet/endothelial JJ 198 202 cell cell NN 203 211 adhesion adhesion NN 212 222 molecule-1 molecule-1 NN 223 224 ( ( ( 224 231 PECAM-1 pecam-1 NN 231 232 ) ) ) 233 235 is be VBZ 236 237 a a DT 238 244 130-kD 130-kd JJ 245 251 member member NN 252 254 of of IN 255 258 the the DT 259 261 Ig Ig NNP 262 266 gene gene NN 267 278 superfamily superfamily NN 279 283 that that WDT 284 286 is be VBZ 287 296 expressed express VBN 297 299 on on IN 300 309 platelets platelet NNS 309 310 , , , 311 322 endothelial endothelial JJ 323 328 cells cell NNS 328 329 , , , 330 333 and and CC 334 341 certain certain JJ 342 351 leukocyte leukocyte NN 352 359 subsets subset NNS 359 360 . . . 361 363 To to TO 364 371 examine examine VB 372 375 the the DT 376 383 factors factor NNS 384 395 controlling control VBG 396 413 vascular-specific vascular-specific JJ 414 424 expression expression NN 425 427 of of IN 428 435 PECAM-1 pecam-1 NN 435 436 , , , 437 439 we we PRP 440 446 cloned clone VBD 447 450 the the DT 451 462 5’-flanking 5'-flanking JJ 463 469 region region NN 470 472 of of IN 473 476 the the DT 477 484 PECAM-1 pecam-1 NN 485 489 gene gene NN 490 493 and and CC 494 502 analyzed analyze VBD 503 506 its its PRP$ 507 522 transcriptional transcriptional JJ 523 531 activity activity NN 531 532 . . . 533 541 5’-Rapid 5'-rapid JJ 542 555 amplification amplification NN 556 558 of of IN 559 563 cDNA cdna NN 564 568 ends end NNS 569 570 ( ( ( 570 577 5’-RACE 5'-RACE NNP 577 578 ) ) ) 579 587 analysis analysis NN 588 594 showed show VBD 595 599 that that IN 600 613 transcription transcription NN 614 624 initiation initiation NN 625 633 occurred occur VBD 634 636 at at IN 637 644 several several JJ 645 652 closely closely RB 653 659 spaced spaced JJ 660 666 nearby nearby JJ 667 672 sites site NNS 673 684 originating originate VBG 685 698 approximately approximately RB 699 702 204 204 CD 703 705 bp bp NN 706 714 upstream upstream RB 715 719 from from IN 720 723 the the DT 724 735 translation translation NN 736 741 start start NN 742 746 site site NN 746 747 . . . 748 756 Analysis Analysis NNP 757 759 of of IN 760 763 the the DT 764 772 sequence sequence NN 773 784 immediately immediately RB 785 793 upstream upstream JJ 794 798 from from IN 799 802 the the DT 803 816 transcription transcription NN 817 827 initiation initiation NN 828 832 site site NN 833 834 ( ( ( 834 837 TIS TIS NNP 837 838 ) ) ) 839 845 showed show VBD 846 848 no no DT 849 858 canonical canonical JJ 859 863 TATA TATA NNP 864 866 or or CC 867 871 CAAT CAAT NNP 872 880 elements element NNS 880 881 , , , 882 889 however however RB 890 892 an an DT 893 902 initiator initiator NN 903 910 element element NN 911 919 commonly commonly RB 920 925 found find VBN 926 928 in in IN 929 938 TATA-less tata-less JJ 939 948 promoters promoter NNS 949 960 encompassed encompass VBD 961 964 the the DT 965 968 TIS tis NN 968 969 . . . 970 981 5’-serially 5'-serially RB 982 991 truncated truncate VBN 992 999 PECAM-1 pecam-1 NN 1000 1008 promoter promoter NN 1009 1017 segments segment NNS 1018 1024 cloned clone VBN 1025 1027 in in IN 1028 1033 front front NN 1034 1036 of of IN 1037 1038 a a DT 1039 1049 luciferase luciferase NN 1050 1058 reporter reporter NN 1059 1064 drove drive VBD 1065 1078 transcription transcription NN 1079 1081 in in IN 1082 1086 both both CC 1087 1088 a a DT 1089 1097 lineage- lineage- NN 1098 1101 and and CC 1102 1122 orientation-specific orientation-specific JJ 1123 1129 manner manner NN 1129 1130 . . . 1131 1139 Putative putative JJ 1140 1150 cis-acting cis-acting JJ 1151 1158 control control NN 1159 1167 elements element NNS 1168 1175 present present JJ 1176 1182 within within IN 1183 1184 a a DT 1185 1191 300-bp 300-bp JJ 1192 1196 core core NN 1197 1205 promoter promoter NN 1206 1214 included include VBD 1215 1218 two two CD 1219 1222 ets ets NN 1223 1228 sites site NNS 1228 1229 , , , 1230 1232 an an DT 1233 1236 Sp1 sp1 NN 1237 1241 site site NN 1241 1242 , , , 1243 1249 tandem tandem JJ 1250 1255 E-box e-box NN 1256 1263 domains domain NNS 1263 1264 , , , 1265 1268 two two CD 1269 1284 GATA-associated gata-associated JJ 1285 1290 sites site NNS 1291 1292 ( ( ( 1292 1297 CACCC CACCC NNP 1297 1298 ) ) ) 1298 1299 , , , 1300 1302 an an DT 1303 1307 AP-2 ap-2 NN 1308 1315 binding binding NN 1316 1320 site site NN 1320 1321 , , , 1322 1325 and and CC 1326 1327 a a DT 1328 1332 GATA GATA NNP 1333 1340 element element NN 1341 1343 at at IN 1344 1347 -24 -24 CD 1347 1348 . . . 1349 1359 Mutational mutational JJ 1360 1368 analysis analysis NN 1369 1375 showed show VBD 1376 1380 that that IN 1381 1388 optimal optimal JJ 1389 1404 transcriptional transcriptional JJ 1405 1413 activity activity NN 1414 1422 required require VBD 1423 1426 the the DT 1427 1431 GATA GATA NNP 1432 1440 sequence sequence NN 1441 1443 at at IN 1444 1452 position position NN 1453 1456 -24 -24 CD 1456 1457 , , , 1458 1461 and and CC 1462 1471 gel-shift gel-shift JJ 1472 1478 assays assay NNS 1479 1486 further further RB 1487 1493 showed show VBD 1494 1498 that that IN 1499 1502 the the DT 1503 1509 GATA-2 gata-2 NN 1510 1523 transcription transcription NN 1524 1530 factor factor NN 1530 1531 , , , 1532 1535 but but CC 1536 1539 not not RB 1540 1546 GATA-1 gata-1 NN 1546 1547 , , , 1548 1553 bound bind VBD 1554 1556 to to TO 1557 1561 this this DT 1562 1568 region region NN 1569 1571 of of IN 1572 1575 the the DT 1576 1583 PECAM-1 pecam-1 NN 1584 1592 promoter promoter NN 1592 1593 . . . 1594 1607 Understanding understand VBG 1608 1611 the the DT 1612 1616 cis- cis- JJ 1617 1620 and and CC 1621 1632 transacting transact VBG 1633 1640 factors factor NNS 1641 1645 that that WDT 1646 1654 regulate regulate VBP 1655 1658 the the DT 1659 1674 tissue-specific tissue-specific JJ 1675 1685 expression expression NN 1686 1688 of of IN 1689 1696 PECAM-1 PECAM-1 NNP 1697 1703 should should MD 1704 1712 increase increase VB 1713 1716 our our PRP$ 1717 1730 understanding understanding NN 1731 1733 of of IN 1734 1737 the the DT 1738 1748 mechanisms mechanism NNS 1749 1751 by by IN 1752 1757 which which WDT 1758 1775 vascular-specific vascular-specific JJ 1776 1780 gene gene NN 1781 1791 expression expression NN 1792 1794 is be VBZ 1795 1803 achieved achieve VBN 1803 1804 . . .