0 1 A a DT 2 3 T t NN 4 17 cell-specific cell-specific JJ 18 26 enhancer enhancer NN 27 29 in in IN 30 33 the the DT 34 47 interleukin-3 interleukin-3 NN 48 53 locus locus NN 54 56 is be VBZ 57 66 activated activate VBN 67 80 cooperatively cooperatively RB 81 83 by by IN 84 87 Oct Oct NNP 88 91 and and CC 92 96 NFAT NFAT NNP 97 105 elements element NNS 106 112 within within IN 113 114 a a DT 115 120 DNase DNase NNP 121 137 I-hypersensitive i-hypersensitive JJ 138 142 site site NN 142 143 . . . 145 158 Interleukin-3 interleukin-3 NN 159 160 ( ( ( 160 164 IL-3 IL-3 NNP 164 165 ) ) ) 166 168 is be VBZ 169 170 a a DT 171 179 cytokine cytokine NN 180 184 that that WDT 185 187 is be VBZ 188 197 expressed express VBN 198 207 primarily primarily RB 208 210 in in IN 211 220 activated activate VBN 221 222 T t NN 223 228 cells cell NNS 228 229 . . . 230 234 Here here RB 235 237 we we PRP 238 248 identified identify VBD 249 251 an an DT 252 261 inducible inducible JJ 262 263 T t NN 264 277 cell-specific cell-specific JJ 278 286 enhancer enhancer NN 287 289 14 14 CD 290 292 kb kb NN 293 301 upstream upstream RB 302 304 of of IN 305 308 the the DT 309 313 IL-3 il-3 NN 314 318 gene gene NN 319 323 that that WDT 324 333 responded respond VBD 334 336 to to TO 337 347 activation activation NN 348 350 of of IN 351 352 T t NN 353 357 cell cell NN 358 366 receptor receptor NN 367 376 signaling signaling NN 377 385 pathways pathway NNS 385 386 . . . 387 390 The the DT 391 395 IL-3 il-3 NN 396 404 enhancer enhancer NN 405 412 spanned span VBD 413 415 an an DT 416 425 inducible inducible JJ 426 437 cyclosporin cyclosporin NN 438 449 A-sensitive a-sensitive JJ 450 455 DNase DNase NNP 456 472 I-hypersensitive i-hypersensitive JJ 473 477 site site NN 478 483 found find VBN 484 488 only only RB 489 491 in in IN 492 493 T t NN 494 499 cells cell NNS 499 500 . . . 501 505 Four four CD 506 515 NFAT-like nfat-like JJ 516 524 elements element NNS 525 530 exist exist VBP 531 537 within within IN 538 541 the the DT 542 550 enhancer enhancer NN 550 551 . . . 552 555 The the DT 556 559 two two CD 560 564 most most RBS 565 571 active active JJ 572 581 NFAT-like nfat-like JJ 582 590 elements element NNS 591 595 were be VBD 596 603 located located JJ 604 606 at at IN 607 610 the the DT 611 617 center center NN 618 620 of of IN 621 624 the the DT 625 630 DNase DNase NNP 631 647 I-hypersensitive i-hypersensitive JJ 648 652 site site NN 652 653 . . . 654 657 One one CD 658 660 of of IN 661 666 these these DT 667 676 NFAT-like nfat-like JJ 677 685 elements element NNS 686 697 encompassed encompass VBD 698 709 overlapping overlap VBG 710 714 Oct- oct- NN 715 718 and and CC 719 734 NFATp/c-binding nfatp/c-binding JJ 735 740 sites site NNS 740 741 , , , 742 747 which which WDT 748 758 functioned function VBD 759 761 in in IN 762 763 a a DT 764 770 highly highly RB 771 782 synergistic synergistic JJ 783 789 manner manner NN 789 790 . . . 791 793 We we PRP 794 801 suggest suggest VBP 802 806 that that IN 807 810 the the DT 811 812 T t NN 813 826 cell-specific cell-specific JJ 827 837 expression expression NN 838 840 of of IN 841 844 the the DT 845 849 IL-3 il-3 NN 850 854 gene gene NN 855 857 is be VBZ 858 864 partly partly RB 865 875 controlled control VBN 876 883 through through IN 884 887 the the DT 888 896 enhancer enhancer NN 897 899 by by IN 900 911 cooperation cooperation NN 912 919 between between IN 920 923 Oct Oct NNP 924 927 and and CC 928 932 NFAT NFAT NNP 933 939 family family NN 940 948 proteins protein NNS 948 949 . . .