0 7 Nuclear nuclear JJ 8 13 Rel-A Rel-A NNP 14 17 and and CC 18 23 c-Rel c-rel NN 24 31 protein protein NN 32 41 complexes complex NNS 42 45 are be VBP 46 60 differentially differentially RB 61 72 distributed distribute VBN 73 79 within within IN 80 85 human human JJ 86 96 thymocytes thymocyte NNS 96 97 . . . 99 106 Nuclear nuclear JJ 107 119 factor-kappa factor-kappa NN 120 121 B B NNP 122 123 ( ( ( 123 131 NF-kappa NF-kappa NNP 132 138 B)/Rel B)/Rel NNP 139 147 proteins protein NNS 148 151 are be VBP 152 161 inducible inducible JJ 162 177 transcriptional transcriptional JJ 178 188 regulators regulator NNS 189 191 of of IN 192 200 numerous numerous JJ 201 209 cellular cellular JJ 210 215 genes gene NNS 215 216 . . . 217 221 They they PRP 222 225 are be VBP 226 238 particularly particularly RB 239 247 abundant abundant JJ 248 250 in in IN 251 259 lymphoid lymphoid JJ 260 267 tissues tissue NNS 268 271 and and CC 272 275 are be VBP 276 283 thought think VBN 284 286 to to TO 287 289 be be VB 290 298 critical critical JJ 299 302 for for IN 303 306 the the DT 307 320 transcription transcription NN 321 323 of of IN 324 329 genes gene NNS 330 338 involved involve VBN 339 341 in in IN 342 348 immune immune JJ 349 352 and and CC 353 365 inflammatory inflammatory JJ 366 375 responses response NNS 375 376 . . . 377 379 We we PRP 380 384 have have VBP 385 393 reported report VBN 394 404 previously previously RB 405 409 that that IN 410 411 a a DT 412 419 nuclear nuclear JJ 420 428 NF-kappa NF-kappa NNP 429 430 B B NNP 431 439 activity activity NN 440 443 was be VBD 444 451 present present JJ 452 454 in in IN 455 462 freshly freshly RB 463 472 extracted extract VBN 473 478 human human JJ 479 489 thymocytes thymocyte NNS 490 492 in in IN 493 496 the the DT 497 504 absence absence NN 505 507 of of IN 508 510 in in FW 511 516 vitro vitro FW 517 526 treatment treatment NN 527 529 of of IN 530 535 these these DT 536 541 cells cell NNS 541 542 . . . 543 545 In in IN 546 549 the the DT 550 557 present present JJ 558 564 report report NN 564 565 , , , 566 568 we we PRP 569 579 identified identify VBD 580 588 NF-kappa NF-kappa NNP 589 590 B B NNP 591 599 proteins protein NNS 600 609 extracted extract VBN 610 614 from from IN 615 620 human human JJ 621 630 thymocyte thymocyte NN 631 637 nuclei nucleus NNS 638 640 as as IN 641 646 being be VBG 647 654 p50/p65 p50/p65 NN 655 658 and and CC 659 668 p50/c-Rel p50/c-rel NN 669 678 complexes complex NNS 678 679 . . . 680 694 Immunochemical immunochemical JJ 695 698 and and CC 699 716 immunofluorescent immunofluorescent JJ 717 725 staining staining NN 726 728 of of IN 729 735 thymus thymus NN 736 744 sections section NNS 745 750 using use VBG 751 759 specific specific JJ 760 763 Abs ab NNS 764 771 allowed allow VBD 772 785 visualization visualization NN 786 788 of of IN 789 796 nuclear nuclear JJ 797 805 NF-kappa NF-kappa NNP 806 807 B B NNP 808 816 proteins protein NNS 817 819 in in IN 820 824 both both CC 825 835 thymocytes thymocyte NNS 836 839 and and CC 840 852 nonthymocyte nonthymocyte JJ 853 858 cells cell NNS 858 859 . . . 860 864 This this DT 865 874 detection detection NN 875 884 suggested suggest VBD 885 886 a a DT 887 899 preferential preferential JJ 900 910 activation activation NN 911 913 of of IN 914 923 p50/c-Rel p50/c-rel NN 924 926 in in IN 927 936 medullary medullary JJ 937 947 thymocytes thymocyte NNS 947 948 , , , 949 956 whereas whereas IN 957 964 p50/p65 p50/p65 NN 965 968 was be VBD 969 976 present present JJ 977 979 in in IN 980 984 both both CC 985 993 cortical cortical JJ 994 997 and and CC 998 1007 medullary medullary JJ 1008 1015 regions region NNS 1016 1018 of of IN 1019 1024 human human JJ 1025 1031 thymus thymus NN 1032 1039 lobules lobule NNS 1039 1040 . . . 1041 1048 However however RB 1048 1049 , , , 1050 1053 the the DT 1054 1063 intensity intensity NN 1064 1066 of of IN 1067 1070 p65 p65 NN 1071 1079 labeling labeling NN 1080 1083 was be VBD 1084 1088 much much RB 1089 1095 higher high JJR 1096 1098 in in IN 1099 1106 several several JJ 1107 1117 thymocytes thymocyte NNS 1118 1122 from from IN 1123 1126 the the DT 1127 1135 medulla. medulla. NN 1136 1139 p65 p65 NN 1139 1140 , , , 1141 1144 p50 p50 NN 1144 1145 , , , 1146 1149 and and CC 1150 1155 c-Rel c-rel NN 1156 1166 activities activity NNS 1167 1171 were be VBD 1172 1177 found find VBN 1178 1180 in in IN 1181 1185 both both CC 1186 1190 CD4- cd4- JJ 1191 1194 and and CC 1195 1207 CD8-positive cd8-positive JJ 1208 1218 thymocytes thymocyte NNS 1218 1219 . . . 1220 1225 These these DT 1226 1238 observations observation NNS 1239 1246 suggest suggest VBP 1247 1251 that that IN 1252 1255 p65 p65 NN 1256 1259 and and CC 1260 1265 c-Rel c-rel NN 1266 1275 complexes complex NNS 1276 1280 play play VBP 1281 1289 distinct distinct JJ 1290 1295 roles role NNS 1296 1298 in in IN 1299 1303 gene gene NN 1304 1314 expression expression NN 1315 1318 and and CC 1319 1323 that that IN 1324 1328 both both DT 1329 1334 forms form NNS 1335 1337 of of IN 1338 1346 NF-kappa NF-kappa NNP 1347 1348 B B NNP 1349 1353 play play VBP 1354 1362 critical critical JJ 1363 1368 roles role NNS 1369 1375 during during IN 1376 1380 late late JJ 1381 1387 stages stage NNS 1388 1390 of of IN 1391 1394 the the DT 1395 1406 intrathymic intrathymic JJ 1407 1417 maturation maturation NN 1418 1420 of of IN 1421 1422 T t NN 1423 1428 cells cell NNS 1428 1429 . . .