0	10	Alteration	Alteration	NNP
11	13	of	of	IN
14	15	a	a	DT
16	22	single	single	JJ
23	29	serine	serine	NN
30	32	in	in	IN
33	36	the	the	DT
37	42	basic	basic	JJ
43	49	domain	domain	NN
50	52	of	of	IN
53	56	the	the	DT
57	69	Epstein-Barr	epstein-barr	JJ
70	75	virus	virus	NN
76	81	ZEBRA	ZEBRA	NNP
82	89	protein	protein	NN
90	99	separates	separate	VBZ
100	103	its	its	PRP$
104	113	functions	function	NNS
114	116	of	of	IN
117	132	transcriptional	transcriptional	JJ
133	143	activation	activation	NN
144	147	and	and	CC
148	158	disruption	disruption	NN
159	161	of	of	IN
162	169	latency	latency	NN
169	170	.	.	.

172	175	The	the	DT
176	181	ZEBRA	ZEBRA	NNP
182	189	protein	protein	NN
190	194	from	from	IN
195	207	Epstein-Barr	epstein-barr	JJ
208	213	virus	virus	NN
214	215	(	(	(
215	218	EBV	EBV	NNP
218	219	)	)	)
220	229	activates	activate	VBZ
230	231	a	a	DT
232	238	switch	switch	NN
239	243	from	from	IN
244	247	the	the	DT
248	254	latent	latent	JJ
255	257	to	to	TO
258	261	the	the	DT
262	267	lytic	lytic	JJ
268	278	expression	expression	NN
279	286	program	program	NN
287	289	of	of	IN
290	293	the	the	DT
294	299	virus	virus	NN
299	300	.	.	.

301	306	ZEBRA	ZEBRA	NNP
306	307	,	,	,
308	309	a	a	DT
310	316	member	member	NN
317	319	of	of	IN
320	323	the	the	DT
324	328	bZIP	bzip	NN
329	335	family	family	NN
336	338	of	of	IN
339	350	DNA-binding	dna-binding	JJ
351	359	proteins	protein	NNS
359	360	,	,	,
361	363	is	be	VBZ
364	365	a	a	DT
366	381	transcriptional	transcriptional	JJ
382	391	activator	activator	NN
392	399	capable	capable	JJ
400	402	of	of	IN
403	411	inducing	induce	VBG
412	422	expression	expression	NN
423	427	from	from	IN
428	433	viral	viral	JJ
434	439	lytic	lytic	JJ
440	445	cycle	cycle	NN
446	455	promoters	promoter	NNS
455	456	.	.	.

457	459	It	it	PRP
460	463	had	have	VBD
464	474	previously	previously	RB
475	479	been	be	VBN
480	487	thought	think	VBN
488	492	that	that	IN
493	498	ZEBRA	ZEBRA	NNP
498	500	’s	's	POS
501	509	capacity	capacity	NN
510	512	to	to	TO
513	520	disrupt	disrupt	VB
521	524	EBV	EBV	NNP
525	532	latency	latency	NN
533	540	resided	reside	VBD
541	550	primarily	primarily	RB
551	553	in	in	IN
554	557	its	its	PRP$
558	565	ability	ability	NN
566	568	to	to	TO
569	577	activate	activate	VB
578	591	transcription	transcription	NN
592	594	of	of	IN
595	600	genes	gene	NNS
601	605	that	that	WDT
606	612	encode	encode	VBP
613	621	products	product	NNS
622	630	required	require	VBN
631	634	for	for	IN
635	640	lytic	lytic	JJ
641	652	replication	replication	NN
652	653	.	.	.

654	656	We	we	PRP
657	666	generated	generate	VBD
667	668	a	a	DT
669	674	point	point	NN
675	681	mutant	mutant	NN
682	684	of	of	IN
685	690	ZEBRA	ZEBRA	NNP
690	691	,	,	,
692	700	Z(S186A)	Z(S186A)	NNP
700	701	,	,	,
702	706	that	that	WDT
707	710	was	be	VBD
711	714	not	not	RB
715	723	impaired	impaired	JJ
724	726	in	in	IN
727	730	its	its	PRP$
731	738	ability	ability	NN
739	741	to	to	TO
742	750	activate	activate	VB
751	764	transcription	transcription	NN
764	765	;	;	:
766	773	however	however	RB
773	774	,	,	,
775	779	this	this	DT
780	788	mutation	mutation	NN
789	798	abolished	abolish	VBD
799	802	its	its	PRP$
803	810	ability	ability	NN
811	813	to	to	TO
814	822	initiate	initiate	VB
823	826	the	the	DT
827	832	viral	viral	JJ
833	838	lytic	lytic	JJ
839	846	cascade	cascade	NN
846	847	.	.	.

848	851	The	the	DT
852	858	mutant	mutant	NN
858	859	,	,	,
860	870	containing	contain	VBG
871	872	a	a	DT
873	890	serine-to-alanine	serine-to-alanine	JJ
891	903	substitution	substitution	NN
904	906	in	in	IN
907	910	the	the	DT
911	922	DNA-binding	dna-binding	JJ
923	929	domain	domain	NN
930	932	of	of	IN
933	936	the	the	DT
937	944	protein	protein	NN
944	945	,	,	,
946	951	bound	bind	VBD
952	954	to	to	TO
955	962	several	several	JJ
963	968	known	know	VBN
969	982	ZEBRA-binding	zebra-binding	JJ
983	988	sites	site	NNS
989	992	and	and	CC
993	1002	activated	activate	VBD
1003	1016	transcription	transcription	NN
1017	1021	from	from	IN
1022	1031	reporters	reporter	NNS
1032	1039	bearing	bear	VBG
1040	1045	known	known	JJ
1046	1062	ZEBRA-responsive	zebra-responsive	JJ
1063	1072	promoters	promoter	NNS
1073	1076	but	but	CC
1077	1080	did	do	VBD
1081	1084	not	not	RB
1085	1092	disrupt	disrupt	VB
1093	1100	latency	latency	NN
1101	1103	in	in	IN
1104	1116	EBV-infected	ebv-infected	JJ
1117	1121	cell	cell	NN
1122	1127	lines	line	NNS
1127	1128	.	.	.

1129	1138	Therefore	therefore	RB
1138	1139	,	,	,
1140	1150	initiation	initiation	NN
1151	1153	of	of	IN
1154	1157	the	the	DT
1158	1161	EBV	EBV	NNP
1162	1167	lytic	lytic	JJ
1168	1173	cycle	cycle	NN
1174	1176	by	by	IN
1177	1180	the	the	DT
1181	1186	ZEBRA	ZEBRA	NNP
1187	1194	protein	protein	NN
1195	1203	requires	require	VBZ
1204	1205	a	a	DT
1206	1214	function	function	NN
1215	1217	in	in	IN
1218	1226	addition	addition	NN
1227	1229	to	to	TO
1230	1245	transcriptional	transcriptional	JJ
1246	1256	activation	activation	NN
1256	1257	;	;	:
1258	1259	a	a	DT
1260	1266	change	change	NN
1267	1269	of	of	IN
1270	1276	serine	serine	NN
1277	1280	186	186	CD
1281	1283	to	to	TO
1284	1291	alanine	alanine	NN
1292	1294	in	in	IN
1295	1298	the	the	DT
1299	1310	DNA-binding	dna-binding	JJ
1311	1317	domain	domain	NN
1318	1320	of	of	IN
1321	1326	ZEBRA	ZEBRA	NNP
1327	1336	abolished	abolish	VBD
1337	1341	this	this	DT
1342	1352	additional	additional	JJ
1353	1361	function	function	NN
1362	1365	and	and	CC
1366	1375	uncovered	uncover	VBD
1376	1377	a	a	DT
1378	1381	new	new	JJ
1382	1386	role	role	NN
1387	1390	for	for	IN
1391	1394	the	the	DT
1395	1400	ZEBRA	ZEBRA	NNP
1401	1408	protein	protein	NN
1409	1411	in	in	IN
1412	1422	disruption	disruption	NN
1423	1425	of	of	IN
1426	1429	EBV	EBV	NNP
1430	1437	latency	latency	NN
1437	1438	.	.	.

1439	1442	The	the	DT
1443	1453	additional	additional	JJ
1454	1462	function	function	NN
1463	1467	that	that	WDT
1468	1470	is	be	VBZ
1471	1479	required	require	VBN
1480	1483	for	for	IN
1484	1494	initiation	initiation	NN
1495	1497	of	of	IN
1498	1501	the	the	DT
1502	1507	lytic	lytic	JJ
1508	1513	viral	viral	JJ
1514	1518	life	life	NN
1519	1524	cycle	cycle	NN
1525	1527	is	be	VBZ
1528	1534	likely	likely	JJ
1535	1537	to	to	TO
1538	1545	require	require	VB
1546	1561	phosphorylation	phosphorylation	NN
1562	1564	of	of	IN
1565	1571	serine	serine	NN
1572	1575	186	186	CD
1576	1578	of	of	IN
1579	1582	the	the	DT
1583	1588	ZEBRA	ZEBRA	NNP
1589	1596	protein	protein	NN
1596	1597	,	,	,
1598	1603	which	which	WDT
1604	1607	may	may	MD
1608	1617	influence	influence	VB
1618	1624	either	either	CC
1625	1628	DNA	dna	NN
1629	1640	recognition	recognition	NN
1641	1643	or	or	CC
1644	1659	transcriptional	transcriptional	JJ
1660	1670	activation	activation	NN
1671	1673	of	of	IN
1674	1679	lytic	lytic	JJ
1680	1685	viral	viral	JJ
1686	1695	promoters	promoter	NNS
1696	1698	in	in	IN
1699	1700	a	a	DT
1701	1714	chromatinized	chromatinize	VBN
1715	1720	viral	viral	JJ
1721	1728	episome	episome	NN
1728	1729	.	.	.