0 10 Activation Activation NNP 11 13 of of IN 14 17 the the DT 18 31 transcription transcription NN 32 38 factor factor NN 39 48 NF-kappaB nf-kappab NN 49 51 in in IN 52 81 lipopolysaccharide-stimulated lipopolysaccharide-stimulated JJ 82 86 U937 u937 NN 87 92 cells cell NNS 92 93 . . . 95 101 During during IN 102 105 the the DT 106 112 course course NN 113 115 of of IN 116 123 serious serious JJ 124 133 bacterial bacterial JJ 134 144 infections infection NNS 144 145 , , , 146 164 lipopolysaccharide lipopolysaccharide NN 165 166 ( ( ( 166 169 LPS LPS NNP 169 170 ) ) ) 171 180 interacts interact VBZ 181 185 with with IN 186 205 monocyte/macrophage monocyte/macrophage NN 206 215 receptors receptor NNS 215 216 , , , 217 226 resulting result VBG 227 229 in in IN 230 233 the the DT 234 244 generation generation NN 245 247 of of IN 248 260 inflammatory inflammatory JJ 261 270 cytokines cytokine NNS 270 271 . . . 272 285 Transcription Transcription NNP 286 292 factor factor NN 293 302 NF-kappaB NF-kappaB NNP 303 305 is be VBZ 306 313 crucial crucial JJ 314 316 in in IN 317 327 activating activate VBG 328 331 the the DT 332 345 transcription transcription NN 346 348 of of IN 349 354 genes gene NNS 355 363 encoding encode VBG 364 379 proinflammatory proinflammatory JJ 380 389 cytokines cytokine NNS 389 390 . . . 391 393 In in IN 394 398 this this DT 399 404 paper paper NN 404 405 , , , 406 408 we we PRP 409 420 demonstrate demonstrate VBP 421 425 that that IN 426 429 the the DT 430 440 activation activation NN 441 443 of of IN 444 453 NF-kappaB NF-kappaB NNP 454 456 by by IN 457 460 LPS LPS NNP 461 463 in in IN 464 465 a a DT 466 478 promonocytic promonocytic JJ 479 483 cell cell NN 484 488 line line NN 489 490 ( ( ( 490 494 U937 u937 NN 494 495 ) ) ) 496 504 followed follow VBD 505 506 a a DT 507 513 rather rather RB 514 518 slow slow JJ 519 527 kinetics kinetic NNS 527 528 , , , 529 538 depending depend VBG 539 541 on on IN 542 545 the the DT 546 550 rate rate NN 551 553 of of IN 554 567 IkappaB-alpha IkappaB-alpha NNP 568 577 inhibitor inhibitor NN 578 588 hydrolysis hydrolysis NN 588 589 . . . 590 592 No no DT 593 604 degradation degradation NN 605 607 of of IN 608 612 p105 p105 NN 613 616 and and CC 617 621 p100 p100 NN 622 632 inhibitors inhibitor NNS 633 636 was be VBD 637 645 observed observe VBN 646 651 under under IN 652 657 these these DT 658 668 conditions condition NNS 668 669 . . . 670 673 The the DT 674 686 transduction transduction NN 687 694 pathway pathway NN 695 702 leading lead VBG 703 705 to to TO 706 715 NF-kappaB NF-kappaB NNP 716 726 activation activation NN 727 729 in in IN 730 734 U937 u937 NN 735 740 cells cell NNS 741 749 involved involve VBD 750 753 the the DT 754 767 intracellular intracellular JJ 768 778 generation generation NN 779 781 of of IN 782 790 reactive reactive JJ 791 797 oxygen oxygen NN 798 805 species specie NNS 806 807 ( ( ( 807 810 ROS ROS NNP 810 811 ) ) ) 811 812 , , , 813 815 as as IN 816 828 demonstrated demonstrate VBN 829 831 by by IN 832 835 the the DT 836 847 concomitant concomitant JJ 848 858 inhibitory inhibitory JJ 859 866 effects effect NNS 867 869 of of IN 870 882 antioxidants antioxidant NNS 883 885 on on IN 886 895 NF-kappaB NF-kappaB NNP 896 906 activation activation NN 907 910 and and CC 911 914 the the DT 915 923 emission emission NN 924 926 of of IN 927 928 a a DT 929 940 fluorescent fluorescent JJ 941 946 probe probe NN 947 955 reacting react VBG 956 971 intracellularly intracellularly RB 972 976 with with IN 977 985 hydrogen hydrogen NN 986 994 peroxide peroxide NN 994 995 . . . 996 1000 This this DT 1001 1004 ROS ROS NNP 1005 1012 pathway pathway NN 1013 1016 was be VBD 1017 1021 also also RB 1022 1035 characterized characterize VBN 1036 1038 by by IN 1039 1042 the the DT 1043 1046 use use NN 1047 1049 of of IN 1050 1055 other other JJ 1056 1066 inhibitors inhibitor NNS 1066 1067 . . . 1068 1072 This this DT 1073 1080 finding finding NN 1081 1090 indicates indicate VBZ 1091 1095 that that IN 1096 1109 phospholipase phospholipase NN 1110 1112 A2 a2 NN 1113 1116 and and CC 1117 1131 5-lipoxygenase 5-lipoxygenase NN 1132 1135 are be VBP 1136 1140 also also RB 1141 1149 involved involve VBN 1149 1150 . . . 1151 1158 However however RB 1158 1159 , , , 1160 1163 the the DT 1164 1173 NF-kappaB NF-kappaB NNP 1174 1184 activation activation NN 1185 1192 pathway pathway NN 1193 1202 involving involve VBG 1203 1206 the the DT 1207 1213 acidic acidic JJ 1214 1230 sphingomyelinase sphingomyelinase NN 1231 1233 of of IN 1234 1237 the the DT 1238 1252 endolysosomial endolysosomial JJ 1253 1261 membrane membrane NN 1262 1265 did do VBD 1266 1269 not not RB 1270 1274 seem seem VB 1275 1277 to to TO 1278 1289 participate participate VB 1290 1292 in in IN 1293 1296 the the DT 1297 1308 LPS-induced lps-induced JJ 1309 1318 NF-kappaB NF-kappaB NNP 1319 1329 activation activation NN 1330 1332 in in IN 1333 1337 U937 u937 NN 1338 1343 cells cell NNS 1343 1344 . . .