0 8 Possible possible JJ 9 13 role role NN 14 16 of of IN 17 24 nuclear nuclear JJ 25 37 factor-kappa factor-kappa NN 38 39 B B NNP 40 48 activity activity NN 49 51 in in IN 52 60 germline germline NN 61 62 C C NNP 63 70 epsilon epsilon NN 71 84 transcription transcription NN 85 87 in in IN 88 89 a a DT 90 95 human human JJ 96 103 Burkitt Burkitt NNP 104 112 lymphoma lymphoma NN 113 114 B B NNP 115 119 cell cell NN 120 124 line line NN 124 125 . . . 127 134 Nuclear nuclear JJ 135 147 factor-kappa factor-kappa NN 148 149 B B NNP 150 151 ( ( ( 151 159 NF-kappa NF-kappa NNP 160 161 B B NNP 161 162 ) ) ) 163 168 plays play VBZ 169 170 a a DT 171 176 broad broad JJ 177 181 role role NN 182 184 in in IN 185 189 gene gene NN 190 200 regulation regulation NN 200 201 , , , 202 205 but but CC 206 208 it it PRP 209 211 is be VBZ 212 215 not not RB 216 223 evident evident JJ 224 231 whether whether IN 232 240 NF-kappa NF-kappa NNP 241 242 B B NNP 243 247 acts act VBZ 248 250 as as IN 251 252 a a DT 253 262 messenger messenger NN 263 269 system system NN 270 273 for for IN 274 282 germline germline NN 283 284 C C NNP 285 292 epsilon epsilon NN 293 306 transcription transcription NN 306 307 . . . 308 310 We we PRP 311 317 report report VBP 318 322 here here RB 323 327 that that IN 328 331 the the DT 332 341 signaling signaling NN 342 349 cascade cascade NN 350 359 triggered trigger VBN 360 362 by by IN 363 376 interleukin-4 interleukin-4 NN 377 378 ( ( ( 378 382 IL-4 il-4 NN 382 383 ) ) ) 384 386 or or CC 387 396 anti-CD40 anti-cd40 JJ 397 407 monoclonal monoclonal JJ 408 416 antibody antibody NN 417 418 ( ( ( 418 421 mAb mab NN 421 422 ) ) ) 423 435 participates participate VBZ 436 438 in in IN 439 447 NF-kappa NF-kappa NNP 448 449 B B NNP 450 460 activation activation NN 461 472 responsible responsible JJ 473 476 for for IN 477 485 germline germline NN 486 487 C C NNP 488 495 epsilon epsilon NN 496 509 transcription transcription NN 510 512 in in IN 513 514 a a DT 515 520 human human JJ 521 528 Burkitt Burkitt NNP 529 537 lymphoma lymphoma NN 538 539 B B NNP 540 544 cell cell NN 545 549 line line NN 549 550 , , , 551 556 DND39 DND39 NNP 556 557 . . . 558 562 Both both CC 563 567 IL-4 il-4 NN 568 571 and and CC 572 581 anti-CD40 anti-cd40 JJ 582 585 mAb mab NN 586 593 induced induce VBD 594 604 activation activation NN 605 607 of of IN 608 628 phosphatidylinositol phosphatidylinositol NN 629 637 3-kinase 3-kinase NNP 638 639 ( ( ( 639 649 PI3-kinase PI3-kinase NNP 649 650 ) ) ) 650 651 , , , 652 665 translocation translocation NN 666 668 of of IN 669 670 a a DT 671 675 zeta zeta NN 676 683 isoform isoform NN 684 686 of of IN 687 694 protein protein NN 695 701 kinase kinase NNP 702 703 C C NNP 703 704 , , , 705 708 and and CC 709 716 nuclear nuclear JJ 717 727 expression expression NN 728 730 of of IN 731 739 NF-kappa NF-kappa NNP 740 742 B. B. NNP 743 746 All all DT 747 751 such such JJ 752 758 events event NNS 759 763 were be VBD 764 773 abrogated abrogate VBN 774 776 by by IN 777 786 treatment treatment NN 787 791 with with IN 792 800 LY294002 LY294002 NNP 800 801 , , , 802 803 a a DT 804 812 specific specific JJ 813 822 inhibitor inhibitor NN 823 825 of of IN 826 836 PI3-kinase PI3-kinase NNP 836 837 . . . 838 840 In in IN 841 849 addition addition NN 849 850 , , , 851 870 N-acetyl-L-cysteine N-acetyl-L-cysteine NNP 871 872 ( ( ( 872 875 NAC NAC NNP 875 876 ) ) ) 876 877 , , , 878 879 a a DT 880 886 potent potent JJ 887 898 antioxidant antioxidant NN 898 899 , , , 900 909 decreased decrease VBD 910 918 NF-kappa NF-kappa NNP 919 920 B B NNP 921 931 activation activation NN 932 938 caused cause VBN 939 941 by by IN 942 946 IL-4 il-4 NN 946 947 , , , 948 957 anti-CD40 anti-cd40 JJ 958 961 mAb mab NN 961 962 , , , 963 965 or or CC 966 971 their their PRP$ 972 983 combination combination NN 983 984 . . . 985 988 NAC NAC NNP 989 992 was be VBD 993 997 also also RB 998 1007 effective effective JJ 1008 1010 in in IN 1011 1022 diminishing diminish VBG 1023 1031 germline germline NN 1032 1033 C C NNP 1034 1041 epsilon epsilon NN 1042 1055 transcription transcription NN 1055 1056 , , , 1057 1060 and and CC 1061 1064 its its PRP$ 1065 1072 potency potency NN 1073 1076 was be VBD 1077 1083 higher high JJR 1084 1086 in in IN 1087 1095 cultures culture NNS 1096 1108 costimulated costimulate VBN 1109 1113 with with IN 1114 1118 IL-4 il-4 NN 1119 1122 and and CC 1123 1132 anti-CD40 anti-cd40 JJ 1133 1136 mAb mab NN 1137 1141 than than IN 1142 1144 in in IN 1145 1150 those those DT 1151 1161 stimulated stimulate VBN 1162 1166 with with IN 1167 1171 IL-4 il-4 NN 1172 1177 alone alone RB 1177 1178 . . . 1179 1184 These these DT 1185 1192 results result NNS 1193 1201 indicate indicate VBP 1202 1206 that that IN 1207 1211 IL-4 il-4 NN 1212 1215 and and CC 1216 1224 ligation ligation NN 1225 1227 of of IN 1228 1232 CD40 CD40 NNP 1233 1239 induce induce VBP 1240 1248 NF-kappa NF-kappa NNP 1249 1250 B B NNP 1251 1261 expression expression NN 1262 1265 via via IN 1266 1268 at at IN 1269 1274 least least JJS 1275 1276 a a DT 1277 1286 mechanism mechanism NN 1287 1296 dependent dependent JJ 1297 1299 on on IN 1300 1303 the the DT 1304 1314 PI3-kinase PI3-kinase NNP 1315 1322 pathway pathway NN 1323 1326 and and CC 1327 1334 suggest suggest VBP 1335 1339 that that IN 1340 1348 NF-kappa NF-kappa NNP 1349 1350 B B NNP 1351 1360 sensitive sensitive JJ 1361 1363 to to TO 1364 1367 NAC NAC NNP 1368 1371 may may MD 1372 1376 play play VB 1377 1378 a a DT 1379 1383 role role NN 1384 1386 in in IN 1387 1397 regulating regulate VBG 1398 1406 germline germline NN 1407 1408 C C NNP 1409 1416 epsilon epsilon NN 1417 1430 transcription transcription NN 1430 1431 . . .