0 9 Induction Induction NNP 10 12 of of IN 13 22 relA(p65) relA(p65) NNP 23 26 and and CC 27 28 I i NN 29 34 kappa kappa NN 35 36 B B NNP 37 42 alpha alpha NN 43 50 subunit subunit NN 51 61 expression expression NN 62 68 during during IN 69 84 differentiation differentiation NN 85 87 of of IN 88 93 human human JJ 94 104 peripheral peripheral JJ 105 110 blood blood NN 111 120 monocytes monocyte NNS 121 123 to to TO 124 135 macrophages macrophage NNS 135 136 . . . 138 140 We we PRP 141 150 evaluated evaluate VBD 151 154 the the DT 155 165 expression expression NN 166 169 and and CC 170 173 DNA dna NN 174 181 binding binding NN 182 190 activity activity NN 191 193 of of IN 194 201 nuclear nuclear JJ 202 208 factor factor NN 209 210 ( ( ( 210 219 NF)-kappa NF)-kappa NNP 220 221 B B NNP 222 230 subunits subunit NNS 231 233 in in IN 234 239 human human JJ 240 250 peripheral peripheral JJ 251 256 blood blood NN 257 266 monocytes monocyte NNS 267 270 and and CC 271 273 in in IN 274 290 monocyte-derived monocyte-derived JJ 291 302 macrophages macrophage NNS 303 304 ( ( ( 304 308 MDMs MDM NNP 308 309 ) ) ) 309 310 . . . 311 323 Constitutive constitutive JJ 324 327 DNA DNA NNP 328 335 binding binding NN 336 344 activity activity NN 345 355 consisting consist VBG 356 358 of of IN 359 362 p50 p50 NN 363 373 homodimers homodimer NNS 374 377 was be VBD 378 386 detected detect VBN 387 389 in in IN 390 397 nuclear nuclear JJ 398 406 extracts extract NNS 407 411 from from IN 412 416 both both DT 417 421 cell cell NN 422 427 types type NNS 427 428 . . . 429 431 An an DT 432 442 additional additional JJ 443 450 complex complex NN 451 459 composed compose VBN 460 462 of of IN 463 476 p50/RelA(p65) p50/rela(p65) NN 477 489 heterodimers heterodimer NNS 490 498 appeared appear VBD 499 503 only only RB 504 506 in in IN 507 514 nuclear nuclear JJ 515 523 extracts extract NNS 524 528 from from IN 529 534 7-day 7-day JJ 535 539 MDMs mdm NNS 539 540 . . . 541 551 Immunoblot Immunoblot NNP 552 560 analysis analysis NN 561 567 showed show VBD 568 572 that that IN 573 576 the the DT 577 580 p50 p50 NN 581 588 subunit subunit NN 589 592 was be VBD 593 607 constitutively constitutively RB 608 617 expressed express VBN 618 620 in in IN 621 630 monocytes monocyte NNS 631 634 and and CC 635 639 MDMs mdm NNS 639 640 . . . 641 643 In in IN 644 652 contrast contrast NN 652 653 , , , 654 657 the the DT 658 667 RelA(p65) rela(p65) NN 668 675 subunit subunit NN 676 679 was be VBD 680 686 barely barely RB 687 697 detectable detectable JJ 698 700 in in IN 701 710 monocytes monocyte NNS 710 711 , , , 712 715 but but CC 716 719 its its PRP$ 720 725 level level NN 726 735 increased increase VBD 736 744 markedly markedly RB 745 747 in in IN 748 752 MDMs MDM NNP 752 753 . . . 754 762 Analysis Analysis NNP 763 765 of of IN 766 775 RelA(p65) RelA(p65) NNP 776 780 mRNA mRNA NNP 781 789 revealed reveal VBD 790 794 that that IN 795 798 the the DT 799 808 stability stability NN 809 811 of of IN 812 821 RelA(p65) RelA(p65) NNP 822 826 mRNA mRNA NNP 827 830 was be VBD 831 844 significantly significantly RB 845 851 higher high JJR 852 854 in in IN 855 859 MDMs MDM NNP 859 860 , , , 861 869 compared compare VBN 870 874 with with IN 875 884 monocytes monocyte NNS 884 885 . . . 886 888 In in IN 889 893 MDMs MDM NNP 893 894 , , , 895 897 an an DT 898 910 upregulation upregulation NN 911 913 of of IN 914 915 I i NN 916 921 kappa kappa NN 922 923 B B NNP 924 929 alpha alpha NN 930 939 synthesis synthesis NN 940 942 as as RB 943 947 well well RB 948 950 as as IN 951 954 the the DT 955 965 appearance appearance NN 966 968 of of IN 969 970 a a DT 971 976 novel novel JJ 977 981 M(r) m(r) NN 982 988 40,000 40,000 CD 989 993 form form NN 994 996 of of IN 997 998 I i NN 999 1004 kappa kappa NN 1005 1006 B B NNP 1007 1012 alpha alpha NN 1013 1017 were be VBD 1018 1022 also also RB 1023 1031 observed observe VBN 1031 1032 . . . 1033 1038 These these DT 1039 1046 results result NNS 1047 1054 suggest suggest VBP 1055 1059 that that IN 1060 1070 macrophage macrophage NN 1071 1086 differentiation differentiation NN 1087 1094 results result VBZ 1095 1097 in in IN 1098 1101 the the DT 1102 1112 expression expression NN 1113 1115 of of IN 1116 1122 active active JJ 1123 1136 p50/RelA(p65) p50/rela(p65) NN 1137 1149 heterodimers heterodimer NNS 1150 1154 with with IN 1155 1158 the the DT 1159 1167 capacity capacity NN 1168 1170 to to TO 1171 1179 activate activate VB 1180 1186 target target NN 1187 1191 gene gene NN 1192 1202 expression expression NN 1202 1203 . . . 1204 1207 The the DT 1208 1216 parallel parallel JJ 1217 1226 induction induction NN 1227 1229 of of IN 1230 1231 I i NN 1232 1237 kappa kappa NN 1238 1239 B B NNP 1240 1245 alpha alpha NN 1246 1255 synthesis synthesis NN 1256 1259 may may MD 1260 1265 allow allow VB 1266 1269 for for IN 1270 1273 the the DT 1274 1284 continuous continuous JJ 1285 1293 presence presence NN 1294 1296 of of IN 1297 1298 a a DT 1299 1310 cytoplasmic cytoplasmic JJ 1311 1320 reservoir reservoir NN 1321 1323 of of IN 1324 1337 p50/RelA(p65) p50/rela(p65) NN 1338 1347 complexes complex NNS 1348 1352 that that WDT 1353 1356 are be VBP 1357 1364 readily readily RB 1365 1374 available available JJ 1375 1378 for for IN 1379 1395 inducer-mediated inducer-mediated JJ 1396 1407 stimulation stimulation NN 1407 1408 . . .