0 11 Involvement involvement NN 12 14 of of IN 15 17 an an DT 18 26 SAF-like saf-like JJ 27 40 transcription transcription NN 41 47 factor factor NN 48 50 in in IN 51 54 the the DT 55 65 activation activation NN 66 68 of of IN 69 74 serum serum NN 75 82 amyloid amyloid NN 83 84 A a NN 85 89 gene gene NN 90 92 in in IN 93 112 monocyte/macrophage monocyte/macrophage NN 113 118 cells cell NNS 119 121 by by IN 122 140 lipopolysaccharide lipopolysaccharide NN 140 141 . . . 143 148 Serum Serum NNP 149 156 amyloid amyloid NN 157 158 A a NN 159 160 ( ( ( 160 163 SAA SAA NNP 163 164 ) ) ) 165 168 has have VBZ 169 173 been be VBN 174 180 linked link VBN 181 183 to to TO 184 199 atherosclerosis atherosclerosis NN 200 207 because because IN 208 210 of of IN 211 214 its its PRP$ 215 222 ability ability NN 223 225 to to TO 226 233 remodel remodel VB 234 246 high-density high-density JJ 247 258 lipoprotein lipoprotein NN 259 261 by by IN 262 265 the the DT 266 275 depletion depletion NN 276 278 of of IN 279 293 apolipoprotein apolipoprotein NN 294 296 A1 a1 NN 296 297 , , , 298 301 its its PRP$ 302 309 ability ability NN 310 312 to to TO 313 317 bind bind VB 318 329 cholesterol cholesterol NN 329 330 , , , 331 334 and and CC 335 338 its its PRP$ 339 347 presence presence NN 348 350 in in IN 351 354 the the DT 355 370 atherosclerotic atherosclerotic JJ 371 378 plaques plaque NNS 379 381 of of IN 382 390 coronary coronary JJ 391 394 and and CC 395 402 carotid carotid JJ 403 411 arteries artery NNS 411 412 . . . 413 415 In in IN 416 419 the the DT 420 427 present present JJ 428 433 study study NN 433 434 , , , 435 437 we we PRP 438 450 investigated investigate VBD 451 454 the the DT 455 464 induction induction NN 465 474 mechanism mechanism NN 475 477 of of IN 478 481 SAA SAA NNP 482 486 gene gene NN 487 489 in in IN 490 495 THP-1 thp-1 NN 496 515 monocyte/macrophage monocyte/macrophage NN 516 521 cells cell NNS 522 527 which which WDT 528 532 play play VBP 533 534 a a DT 535 543 critical critical JJ 544 548 role role NN 549 551 in in IN 552 555 the the DT 556 567 development development NN 568 570 of of IN 571 586 atherosclerotic atherosclerotic JJ 587 592 fatty fatty JJ 593 599 streak streak NN 600 603 and and CC 604 610 plaque plaque NN 611 620 formation formation NN 620 621 . . . 622 624 We we PRP 625 628 and and CC 629 635 others other NNS 636 640 have have VBP 641 646 shown show VBN 647 651 that that IN 652 655 SAA SAA NNP 656 660 gene gene NN 661 663 is be VBZ 664 671 induced induce VBN 672 674 in in IN 675 694 monocyte/macrophage monocyte/macrophage NN 695 700 cells cell NNS 701 703 by by IN 704 722 lipopolysaccharide lipopolysaccharide NN 723 724 ( ( ( 724 727 LPS LPS NNP 727 728 ) ) ) 728 729 . . . 730 732 By by IN 733 741 promoter promoter NN 742 750 function function NN 751 759 analysis analysis NN 759 760 , , , 761 763 we we PRP 764 768 show show VBP 769 773 that that IN 774 777 the the DT 778 781 SAA SAA NNP 782 790 promoter promoter NN 791 799 sequence sequence NN 800 807 between between IN 808 812 -280 -280 CD 813 816 and and CC 817 821 -226 -226 CD 822 825 can can MD 826 832 confer confer VB 833 836 LPS LPS NNP 837 851 responsiveness responsiveness NN 851 852 . . . 853 856 Gel Gel NNP 857 872 electrophoretic electrophoretic JJ 873 881 mobility mobility NN 882 887 shift shift NN 888 893 assay assay NN 894 902 detected detect VBD 903 905 an an DT 906 913 induced induce VBN 914 925 DNA-binding dna-binding JJ 926 934 activity activity NN 935 937 in in IN 938 943 these these DT 944 949 cells cell NNS 950 952 in in IN 953 961 response response NN 962 964 to to TO 965 968 LPS LPS NNP 968 969 . . . 970 986 Characterization characterization NN 987 989 of of IN 990 993 the the DT 994 1005 DNA-binding dna-binding JJ 1006 1013 protein protein NN 1014 1016 by by IN 1017 1019 UV UV NNP 1020 1033 cross-linking cross-linking NN 1033 1034 , , , 1035 1047 Southwestern Southwestern NNP 1048 1052 blot blot NN 1052 1053 , , , 1054 1057 and and CC 1058 1066 antibody antibody NN 1067 1086 ablation/supershift ablation/supershift NN 1087 1093 assays assay NNS 1094 1102 revealed reveal VBD 1103 1107 that that IN 1108 1110 it it PRP 1111 1113 is be VBZ 1114 1121 similar similar JJ 1122 1124 to to TO 1125 1126 a a DT 1127 1135 recently recently RB 1136 1144 reported report VBN 1145 1152 nuclear nuclear JJ 1153 1159 factor factor NN 1160 1170 designated designate VBN 1171 1174 SAF SAF NNP 1174 1175 . . . 1176 1181 These these DT 1182 1189 results result NNS 1190 1202 demonstrated demonstrate VBD 1203 1207 that that IN 1208 1220 LPS-mediated lps-mediated JJ 1221 1224 SAA SAA NNP 1225 1229 gene gene NN 1230 1239 induction induction NN 1240 1242 in in IN 1243 1262 monocyte/macrophage monocyte/macrophage NN 1263 1268 cells cell NNS 1269 1271 is be VBZ 1272 1281 primarily primarily RB 1282 1285 due due JJ 1286 1288 to to TO 1289 1292 the the DT 1293 1302 induction induction NN 1303 1305 of of IN 1306 1309 SAF SAF NNP 1310 1318 activity activity NN 1318 1319 . . .