0 8 NF-kappa NF-kappa NNP 9 22 B-independent b-independent JJ 23 34 suppression suppression NN 35 37 of of IN 38 41 HIV HIV NNP 42 52 expression expression NN 53 55 by by IN 56 64 ascorbic ascorbic JJ 65 69 acid acid NN 69 70 . . . 72 80 Ascorbic ascorbic JJ 81 85 acid acid NN 86 87 ( ( ( 87 96 ascorbate ascorbate NN 97 99 or or CC 100 107 vitamin vitamin NN 108 109 C C NNP 109 110 ) ) ) 111 114 has have VBZ 115 119 been be VBN 120 125 shown show VBN 126 128 to to TO 129 137 suppress suppress VB 138 141 the the DT 142 151 induction induction NN 152 154 of of IN 155 158 HIV HIV NNP 159 161 in in IN 162 170 latently latently RB 171 179 infected infected JJ 180 181 T t NN 182 193 lymphocytic lymphocytic JJ 194 199 cells cell NNS 200 209 following follow VBG 210 221 stimulation stimulation NN 222 226 with with IN 227 228 a a DT 229 234 tumor tumor NN 235 243 promoter promoter NN 244 245 ( ( ( 245 248 PMA PMA NNP 248 249 ) ) ) 250 253 and and CC 254 266 inflammatory inflammatory JJ 267 275 cytokine cytokine NN 276 277 ( ( ( 277 286 TNF-alpha TNF-alpha NNP 286 287 ) ) ) 287 288 . . . 289 291 To to TO 292 298 assess assess VB 299 306 whether whether IN 307 311 this this DT 312 322 inhibition inhibition NN 323 326 was be VBD 327 335 mediated mediate VBN 336 339 via via IN 340 350 modulation modulation NN 351 353 of of IN 354 357 the the DT 358 366 cellular cellular JJ 367 380 transcription transcription NN 381 387 factor factor NN 387 388 , , , 389 397 NF-kappa NF-kappa NNP 398 399 B B NNP 399 400 , , , 401 403 we we PRP 404 411 carried carry VBD 412 415 out out RP 416 419 gel gel NN 420 425 shift shift NN 426 434 analysis analysis NN 435 437 on on IN 438 445 nuclear nuclear JJ 446 454 extracts extract NNS 455 463 prepared prepare VBN 464 469 under under IN 470 479 different different JJ 480 490 conditions condition NNS 491 493 of of IN 494 498 cell cell NN 499 510 stimulation stimulation NN 511 513 in in IN 514 517 the the DT 518 526 presence presence NN 527 529 or or CC 530 537 absence absence NN 538 540 of of IN 541 550 ascorbate ascorbate NN 550 551 , , , 552 568 N-acetylcysteine N-acetylcysteine NNP 569 570 ( ( ( 570 573 NAC NAC NNP 573 574 ) ) ) 574 575 , , , 576 578 or or CC 579 589 zidovudine zidovudine NN 590 591 ( ( ( 591 594 AZT AZT NNP 594 595 ) ) ) 595 596 . . . 597 609 Pretreatment Pretreatment NNP 610 612 of of IN 613 618 ACH-2 ach-2 NN 619 620 T t NN 621 626 cells cell NNS 627 629 by by IN 630 633 NAC NAC NNP 634 642 followed follow VBN 643 645 by by IN 646 657 stimulation stimulation NN 658 662 with with IN 663 666 PMA PMA NNP 666 667 , , , 668 677 TNF-alpha TNF-alpha NNP 677 678 , , , 679 681 or or CC 682 690 hydrogen hydrogen NN 691 699 peroxide peroxide NN 700 701 ( ( ( 701 705 H2O2 h2o2 NN 705 706 ) ) ) 707 715 resulted result VBD 716 718 in in IN 719 725 strong strong JJ 726 737 suppression suppression NN 738 740 of of IN 741 749 NF-kappa NF-kappa NNP 750 751 B B NNP 752 762 activation activation NN 762 763 . . . 764 766 In in IN 767 775 contrast contrast NN 775 776 , , , 777 784 neither neither CC 785 794 ascorbate ascorbate NN 795 798 nor nor CC 799 802 AZT AZT NNP 803 811 affected affect VBD 812 820 NF-kappa NF-kappa NNP 821 822 B B NNP 823 831 activity activity NN 832 837 under under IN 838 841 all all DT 842 847 three three CD 848 857 induction induction NN 858 868 conditions condition NNS 869 871 in in IN 872 875 the the DT 876 881 ACH-2 ach-2 NN 882 886 cell cell NN 887 891 line line NN 891 892 . . . 893 902 Ascorbate Ascorbate NNP 903 906 and and CC 907 910 AZT AZT NNP 911 915 also also RB 916 919 had have VBD 920 922 no no DT 923 929 effect effect NN 930 932 on on IN 933 941 NF-kappa NF-kappa NNP 942 943 B B NNP 944 954 activation activation NN 955 964 following follow VBG 965 975 TNF-alpha- tnf-alpha- NN 976 978 or or CC 979 990 PMA-induced pma-induced JJ 991 1002 stimulation stimulation NN 1003 1005 of of IN 1006 1008 U1 u1 NN 1009 1021 promonocytic promonocytic JJ 1022 1027 cells cell NNS 1027 1028 . . . 1029 1034 These these DT 1035 1042 results result NNS 1043 1050 suggest suggest VBP 1051 1055 that that IN 1056 1059 the the DT 1060 1069 molecular molecular JJ 1070 1079 mechanism mechanism NN 1080 1082 of of IN 1083 1086 HIV HIV NNP 1087 1097 inhibition inhibition NN 1098 1100 by by IN 1101 1110 ascorbate ascorbate NN 1111 1113 is be VBZ 1114 1117 not not RB 1118 1126 mediated mediate VBN 1127 1130 via via IN 1131 1139 NF-kappa NF-kappa NNP 1140 1141 B B NNP 1142 1152 inhibition inhibition NN 1152 1153 , , , 1154 1160 unlike unlike IN 1161 1165 that that DT 1166 1170 seen see VBN 1171 1175 with with IN 1176 1181 other other JJ 1182 1194 antioxidants antioxidant NNS 1194 1195 . . .