0 8 Neuronal neuronal JJ 9 10 ( ( ( 10 14 type type NN 15 16 I i CD 16 17 ) ) ) 18 24 nitric nitric JJ 25 30 oxide oxide NN 31 39 synthase synthase NN 40 49 regulates regulate VBZ 50 57 nuclear nuclear JJ 58 64 factor factor NN 65 71 kappaB kappab NN 72 80 activity activity NN 81 84 and and CC 85 96 immunologic immunologic JJ 97 98 ( ( ( 98 102 type type NN 103 105 II ii CD 105 106 ) ) ) 107 113 nitric nitric JJ 114 119 oxide oxide NN 120 128 synthase synthase NN 129 139 expression expression NN 139 140 . . . 142 148 Nitric nitric JJ 149 154 oxide oxide NN 155 164 subserves subserve VBZ 165 172 diverse diverse JJ 173 184 physiologic physiologic JJ 185 190 roles role NNS 191 193 in in IN 194 197 the the DT 198 205 nervous nervous JJ 206 212 system system NN 212 213 . . . 214 216 NO no NN 217 219 is be VBZ 220 228 produced produce VBN 229 233 from from IN 234 236 at at IN 237 242 least least JJS 243 248 three three CD 249 258 different different JJ 259 261 NO no NN 262 270 synthase synthase NN 271 272 ( ( ( 272 275 NOS NOS NNP 275 276 ) ) ) 277 285 isoforms isoform NNS 285 286 : : : 287 295 neuronal neuronal JJ 296 299 NOS nos NN 300 301 ( ( ( 301 305 nNOS nnos NN 305 306 ) ) ) 306 307 , , , 308 319 endothelial endothelial JJ 320 323 NOS nos NN 323 324 , , , 325 328 and and CC 329 340 immunologic immunologic JJ 341 344 NOS nos NN 345 346 ( ( ( 346 350 iNOS inos NN 350 351 ) ) ) 351 352 . . . 353 355 We we PRP 356 360 show show VBP 361 365 that that IN 366 370 nNOS nnos NN 371 373 is be VBZ 374 377 the the DT 378 389 predominant predominant JJ 390 397 isoform isoform NN 398 412 constitutively constitutively RB 413 422 expressed express VBN 423 425 in in IN 426 430 glia glia NN 430 431 . . . 432 434 NO no NN 435 442 derived derive VBN 443 447 from from IN 448 452 nNOS nnos NN 453 455 in in IN 456 460 glia glia NN 461 469 inhibits inhibit VBZ 470 473 the the DT 474 487 transcription transcription NN 488 494 factor factor NN 495 502 nuclear nuclear JJ 503 509 factor factor NN 510 516 kappaB kappaB NNP 517 518 ( ( ( 518 520 NF NF NNP 521 527 kappaB kappaB NNP 527 528 ) ) ) 529 531 as as IN 532 535 NOS NOS NNP 536 546 inhibitors inhibitor NNS 547 554 enhance enhance VBP 555 560 basal basal JJ 561 563 NF NF NNP 564 570 kappaB kappab NN 571 581 activation activation NN 581 582 . . . 583 594 Pyrrolidine Pyrrolidine NNP 595 610 dithiocarbamate dithiocarbamate NN 611 612 ( ( ( 612 616 PDTC PDTC NNP 616 617 ) ) ) 618 620 is be VBZ 621 623 an an DT 624 633 inhibitor inhibitor NN 634 636 of of IN 637 639 NF NF NNP 640 646 kappaB kappaB NNP 647 649 in in IN 650 654 most most JJS 655 660 cells cell NNS 660 661 ; ; : 662 669 however however RB 669 670 , , , 671 673 we we PRP 674 678 show show VBP 679 683 that that IN 684 688 PDTC PDTC NNP 689 691 is be VBZ 692 696 also also RB 697 698 a a DT 699 705 potent potent JJ 706 715 scavenger scavenger NN 716 718 of of IN 719 721 NO no NN 722 729 through through IN 730 739 formation formation NN 740 742 of of IN 743 755 mononitrosyl mononitrosyl NN 756 760 iron iron NN 761 770 complexes complex NNS 771 775 with with IN 776 780 PDTC PDTC NNP 780 781 . . . 782 784 In in IN 785 791 Jurkat Jurkat NNP 792 797 cells cell NNS 797 798 , , , 799 800 a a DT 801 806 human human JJ 807 813 T-cell t-cell NN 814 822 lymphoma lymphoma NN 823 827 cell cell NN 828 832 line line NN 832 833 , , , 834 839 tumor tumor NN 840 848 necrosis necrosis NN 849 861 factor-alpha factor-alpha NN 862 863 ( ( ( 863 872 TNF-alpha TNF-alpha NNP 872 873 ) ) ) 874 881 induces induce VBZ 882 884 NF NF NNP 885 891 kappaB kappab NN 892 902 activation activation NN 903 907 that that WDT 908 910 is be VBZ 911 920 inhibited inhibit VBN 921 923 by by IN 924 928 PDTC PDTC NNP 928 929 . . . 930 938 Contrary contrary JJ 939 941 to to TO 942 945 the the DT 946 953 results result NNS 954 956 in in IN 957 963 Jurkat Jurkat NNP 964 969 cells cell NNS 969 970 , , , 971 975 PDTC PDTC NNP 976 979 did do VBD 980 983 not not RB 984 991 inhibit inhibit VB 992 997 tumor tumor NN 998 1006 necrosis necrosis NN 1007 1027 factor-alpha-induced factor-alpha-induced JJ 1028 1030 NF NF NNP 1031 1037 kappaB kappab NN 1038 1048 activation activation NN 1049 1051 in in IN 1052 1062 astrocytes astrocyte NNS 1062 1063 ; ; : 1064 1071 instead instead RB 1072 1076 PDTC PDTC NNP 1077 1083 itself itself PRP 1084 1091 induces induce VBZ 1092 1094 NF NF NNP 1095 1101 kappaB kappab NN 1102 1112 activation activation NN 1113 1115 in in IN 1116 1126 astrocytes astrocyte NNS 1126 1127 , , , 1128 1131 and and CC 1132 1136 this this DT 1137 1140 may may MD 1141 1143 be be VB 1144 1151 related relate VBN 1152 1154 to to TO 1155 1165 scavenging scavenging NN 1166 1168 of of IN 1169 1181 endogenously endogenously RB 1182 1190 produced produce VBN 1191 1193 NO no NN 1194 1196 by by IN 1197 1200 the the DT 1201 1205 PDTC PDTC NNP 1206 1210 iron iron NN 1211 1218 complex complex NN 1218 1219 . . . 1220 1222 In in IN 1223 1233 astrocytes astrocyte NNS 1234 1238 PDTC PDTC NNP 1239 1243 also also RB 1244 1256 dramatically dramatically RB 1257 1264 induces induce VBZ 1265 1268 the the DT 1269 1271 NF NF NNP 1272 1288 kappaB-dependent kappab-dependent JJ 1289 1295 enzyme enzyme NN 1295 1296 , , , 1297 1301 iNOS inos NN 1301 1302 , , , 1303 1313 supporting support VBG 1314 1317 the the DT 1318 1329 physiologic physiologic JJ 1330 1339 relevance relevance NN 1340 1342 of of IN 1343 1353 endogenous endogenous JJ 1354 1356 NO no NN 1357 1367 regulation regulation NN 1368 1370 of of IN 1371 1373 NF NF NNP 1374 1380 kappaB kappaB NNP 1380 1381 . . . 1382 1384 NF NF NNP 1385 1391 kappaB kappaB NNP 1392 1402 activation activation NN 1403 1405 in in IN 1406 1410 glia glia NN 1411 1415 from from IN 1416 1420 mice mouse NNS 1421 1428 lacking lack VBG 1429 1433 nNOS nnos NN 1434 1442 responds respond VBZ 1443 1447 more more RBR 1448 1455 rapidly rapidly RB 1456 1458 to to TO 1459 1463 PDTC PDTC NNP 1464 1472 compared compare VBN 1473 1477 with with IN 1478 1488 astrocytes astrocyte NNS 1489 1493 from from IN 1494 1503 wild-type wild-type JJ 1504 1508 mice mouse NNS 1508 1509 . . . 1510 1513 Our our PRP$ 1514 1518 data datum NNS 1519 1526 suggest suggest VBP 1527 1531 that that IN 1532 1536 nNOS nnos NN 1537 1539 in in IN 1540 1550 astrocytes astrocyte NNS 1551 1560 regulates regulate VBZ 1561 1563 NF NF NNP 1564 1570 kappaB kappaB NNP 1571 1579 activity activity NN 1580 1583 and and CC 1584 1588 iNOS inos NN 1589 1599 expression expression NN 1599 1600 , , , 1601 1604 and and CC 1605 1613 indicate indicate VBP 1614 1615 a a DT 1616 1621 novel novel JJ 1622 1632 regulatory regulatory JJ 1633 1637 role role NN 1638 1641 for for IN 1642 1646 nNOS nnos NN 1647 1649 in in IN 1650 1659 tonically tonically RB 1660 1671 suppressing suppress VBG 1672 1679 central central JJ 1680 1687 nervous nervous JJ 1688 1694 system system NN 1694 1695 , , , 1696 1698 NF nf NN 1699 1715 kappaB-regulated kappab-regulated JJ 1716 1721 genes gene NNS 1721 1722 . . .