0	3	Itk	Itk	NNP
3	4	,	,	,
5	6	a	a	DT
7	8	T	t	NN
9	22	cell-specific	cell-specific	JJ
23	31	tyrosine	tyrosine	NN
32	38	kinase	kinase	NNP
38	39	,	,	,
40	42	is	be	VBZ
43	51	required	require	VBN
52	55	for	for	IN
56	68	CD2-mediated	cd2-mediated	JJ
69	82	interleukin-2	interleukin-2	NN
83	91	promoter	promoter	NN
92	102	activation	activation	NN
103	105	in	in	IN
106	109	the	the	DT
110	115	human	human	JJ
116	117	T	t	NN
118	122	cell	cell	NN
123	127	line	line	NN
128	134	Jurkat	Jurkat	NNP
134	135	.	.	.

137	139	We	we	PRP
140	152	investigated	investigate	VBD
153	156	the	the	DT
157	167	functional	functional	JJ
168	172	role	role	NN
173	175	of	of	IN
176	179	Itk	Itk	NNP
179	180	,	,	,
181	182	a	a	DT
183	189	member	member	NN
190	192	of	of	IN
193	196	the	the	DT
197	208	cytoplasmic	cytoplasmic	JJ
209	217	tyrosine	tyrosine	NN
218	224	kinase	kinase	NNP
225	228	Tec	Tec	NNP
229	235	family	family	NN
235	236	,	,	,
237	239	in	in	IN
240	241	T	t	NN
242	246	cell	cell	NN
247	257	activation	activation	NN
257	258	.	.	.

259	270	Stimulation	stimulation	NN
271	273	of	of	IN
274	280	either	either	CC
281	284	CD2	cd2	NN
285	287	or	or	CC
288	289	T	t	NN
290	294	cell	cell	NN
295	303	receptor	receptor	NN
304	305	(	(	(
305	313	TCR)/CD3	TCR)/CD3	NNP
314	316	on	on	IN
317	323	Tcells	tcell	NNS
324	326	by	by	IN
327	337	monoclonal	monoclonal	JJ
338	355	antibody-mediated	antibody-mediated	JJ
356	369	cross-linking	cross-linking	NN
370	377	induced	induce	VBD
378	386	tyrosine	tyrosine	NN
387	402	phosphorylation	phosphorylation	NN
403	405	of	of	IN
406	409	Itk	Itk	NNP
409	410	,	,	,
411	416	which	which	WDT
417	420	was	be	VBD
421	428	maximal	maximal	JJ
429	431	as	as	RB
432	437	early	early	RB
438	440	as	as	IN
441	442	1	1	CD
443	446	min	min	NN
447	452	after	after	IN
453	464	stimulation	stimulation	NN
464	465	.	.	.

466	469	The	the	DT
470	478	tyrosine	tyrosine	NN
479	485	kinase	kinase	NNP
486	494	activity	activity	NN
495	497	in	in	IN
498	501	the	the	DT
502	510	anti-Itk	anti-itk	JJ
511	528	immunoprecipitate	immunoprecipitate	NN
529	532	was	be	VBD
533	546	significantly	significantly	RB
547	556	activated	activate	VBN
557	561	upon	upon	IN
562	567	these	these	DT
568	580	stimulations	stimulation	NNS
580	581	.	.	.

582	595	Interleukin-2	interleukin-2	NN
596	597	(	(	(
597	601	IL-2	il-2	NN
601	602	)	)	)
603	611	promoter	promoter	NN
612	620	activity	activity	NN
621	631	stimulated	stimulate	VBN
632	634	by	by	IN
635	648	cross-linking	cross-linking	NN
649	651	of	of	IN
652	655	CD2	cd2	NN
655	656	,	,	,
657	664	TCR/CD3	TCR/CD3	NNP
664	665	,	,	,
666	669	and	and	CC
670	674	CD28	cd28	NN
675	679	with	with	IN
680	690	antibodies	antibody	NNS
691	694	was	be	VBD
695	708	significantly	significantly	RB
709	716	reduced	reduce	VBN
717	719	by	by	IN
720	729	transient	transient	JJ
730	740	expression	expression	NN
741	743	of	of	IN
744	746	an	an	DT
747	750	Itk	Itk	NNP
751	757	mutant	mutant	NN
758	765	lacking	lack	VBG
766	769	the	the	DT
770	776	kinase	kinase	NNP
777	785	activity	activity	NN
785	786	.	.	.

787	790	The	the	DT
791	800	reduction	reduction	NN
801	811	paralleled	parallel	VBD
812	813	a	a	DT
814	822	decrease	decrease	NN
823	825	in	in	IN
826	834	tyrosine	tyrosine	NN
835	850	phosphorylation	phosphorylation	NN
851	853	of	of	IN
854	864	endogenous	endogenous	JJ
865	874	wild-type	wild-type	JJ
875	878	Itk	itk	NN
878	879	.	.	.

880	891	Stimulation	stimulation	NN
892	894	of	of	IN
895	898	CD2	cd2	NN
899	901	or	or	CC
902	909	TCR/CD3	TCR/CD3	NNP
910	917	induced	induce	VBD
918	928	activation	activation	NN
929	931	of	of	IN
932	935	the	the	DT
936	943	nuclear	nuclear	JJ
944	950	factor	factor	NN
951	953	of	of	IN
954	963	activated	activate	VBN
964	965	T	t	NN
966	971	cells	cell	NNS
972	973	(	(	(
973	977	NFAT	NFAT	NNP
977	978	)	)	)
978	979	,	,	,
980	983	the	the	DT
984	991	binding	bind	VBG
992	996	site	site	NN
997	999	of	of	IN
1000	1005	which	which	WDT
1006	1008	is	be	VBZ
1009	1017	included	include	VBN
1018	1020	in	in	IN
1021	1024	the	the	DT
1025	1029	IL-2	il-2	NN
1030	1034	gene	gene	NN
1035	1043	promoter	promoter	NN
1043	1044	.	.	.

1045	1048	The	the	DT
1049	1059	activation	activation	NN
1060	1062	of	of	IN
1063	1067	NFAT	NFAT	NNP
1068	1071	was	be	VBD
1072	1076	also	also	RB
1077	1085	impaired	impair	VBN
1086	1088	by	by	IN
1089	1099	expression	expression	NN
1100	1102	of	of	IN
1103	1106	the	the	DT
1107	1110	Itk	Itk	NNP
1111	1117	mutant	mutant	NN
1117	1118	.	.	.

1119	1124	These	these	DT
1125	1132	results	result	NNS
1133	1144	demonstrate	demonstrate	VBP
1145	1149	that	that	IN
1150	1153	Itk	Itk	NNP
1154	1159	plays	play	VBZ
1160	1161	a	a	DT
1162	1166	role	role	NN
1167	1169	in	in	IN
1170	1174	IL-2	il-2	NN
1175	1185	production	production	NN
1185	1186	,	,	,
1187	1197	indicating	indicate	VBG
1198	1199	a	a	DT
1200	1208	critical	critical	JJ
1209	1220	involvement	involvement	NN
1221	1223	of	of	IN
1224	1227	Itk	Itk	NNP
1228	1230	in	in	IN
1231	1234	the	the	DT
1235	1242	initial	initial	JJ
1243	1248	stage	stage	NN
1249	1251	of	of	IN
1252	1253	T	t	NN
1254	1258	cell	cell	NN
1259	1269	activation	activation	NN
1270	1272	by	by	IN
1273	1282	mediating	mediate	VBG
1283	1290	signals	signal	NNS
1291	1295	from	from	IN
1296	1299	the	the	DT
1300	1307	TCR/CD3	tcr/cd3	NN
1308	1315	complex	complex	NN
1315	1316	,	,	,
1317	1320	CD2	cd2	NN
1320	1321	,	,	,
1322	1325	and	and	CC
1326	1330	CD28	cd28	NN
1330	1331	.	.	.