0	9	Structure	structure	NN
10	13	and	and	CC
14	22	function	function	NN
23	31	analysis	analysis	NN
32	34	of	of	IN
35	38	the	the	DT
39	44	human	human	JJ
45	52	myeloid	myeloid	JJ
53	57	cell	cell	NN
58	65	nuclear	nuclear	JJ
66	81	differentiation	differentiation	NN
82	89	antigen	antigen	NN
90	98	promoter	promoter	NN
98	99	:	:	:
100	108	evidence	evidence	NN
109	112	for	for	IN
113	116	the	the	DT
117	121	role	role	NN
122	124	of	of	IN
125	128	Sp1	Sp1	NNP
129	132	and	and	CC
133	136	not	not	RB
137	139	of	of	IN
140	145	c-Myb	c-myb	NN
146	148	or	or	CC
149	153	PU.1	pu.1	NN
154	156	in	in	IN
157	171	myelomonocytic	myelomonocytic	JJ
172	188	lineage-specific	lineage-specific	JJ
189	199	expression	expression	NN
199	200	.	.	.

202	205	The	the	DT
206	211	human	human	JJ
212	219	myeloid	myeloid	JJ
220	227	nuclear	nuclear	JJ
228	243	differentiation	differentiation	NN
244	251	antigen	antigen	NN
252	253	(	(	(
253	257	MNDA	MNDA	NNP
257	258	)	)	)
259	261	is	be	VBZ
262	271	expressed	express	VBN
272	284	specifically	specifically	RB
285	287	in	in	IN
288	296	maturing	mature	VBG
297	302	cells	cell	NNS
303	305	of	of	IN
306	309	the	the	DT
310	324	myelomonocytic	myelomonocytic	JJ
325	332	lineage	lineage	NN
333	336	and	and	CC
337	339	in	in	IN
340	349	monocytes	monocyte	NNS
350	353	and	and	CC
354	366	granulocytes	granulocyte	NNS
366	367	.	.	.

368	375	Epitope	Epitope	NNP
376	387	enhancement	enhancement	NN
388	391	was	be	VBD
392	396	used	use	VBN
397	399	to	to	TO
400	407	confirm	confirm	VB
408	411	the	the	DT
412	418	strict	strict	JJ
419	427	lineage-	lineage-	NN
428	431	and	and	CC
432	446	stage-specific	stage-specific	JJ
447	457	expression	expression	NN
458	460	of	of	IN
461	465	MNDA	MNDA	NNP
466	468	in	in	IN
469	473	bone	bone	NN
474	480	marrow	marrow	NN
481	483	as	as	RB
484	488	well	well	RB
489	491	as	as	IN
492	494	in	in	IN
495	500	other	other	JJ
501	518	paraffin-embedded	paraffin-embedded	JJ
519	524	fixed	fixed	JJ
525	532	tissues	tissue	NNS
532	533	.	.	.

534	535	A	a	DT
536	540	1-kb	1-kb	JJ
541	547	region	region	NN
548	550	of	of	IN
551	554	the	the	DT
555	559	gene	gene	NN
560	564	that	that	WDT
565	573	includes	include	VBZ
574	575	5	5	CD
575	576	’	'	SYM
577	585	flanking	flanking	NN
586	594	sequence	sequence	NN
595	598	was	be	VBD
599	607	reported	report	VBN
608	615	earlier	earlier	RBR
616	618	to	to	TO
619	626	contain	contain	VB
627	637	functional	functional	JJ
638	646	promoter	promoter	NN
647	655	activity	activity	NN
656	659	and	and	CC
660	663	was	be	VBD
664	676	specifically	specifically	RB
677	689	demethylated	demethylate	VBN
690	692	in	in	IN
693	703	expressing	express	VBG
704	709	cells	cell	NNS
710	712	in	in	IN
713	721	contrast	contrast	NN
722	724	to	to	TO
725	729	null	null	JJ
730	735	cells	cell	NNS
735	736	.	.	.

737	744	Further	further	JJ
745	753	analysis	analysis	NN
754	757	has	have	VBZ
758	766	revealed	reveal	VBN
767	771	that	that	IN
772	776	this	this	DT
777	781	1-kb	1-kb	JJ
782	790	fragment	fragment	NN
791	799	promotes	promote	VBZ
800	806	higher	high	JJR
807	815	reporter	reporter	NN
816	820	gene	gene	NN
821	829	activity	activity	NN
830	832	in	in	IN
833	848	MNDA-expressing	mnda-expressing	JJ
849	854	cells	cell	NNS
855	859	than	than	IN
860	874	non-expressing	non-expressing	JJ
875	880	cells	cell	NNS
880	881	,	,	,
882	892	indicating	indicate	VBG
893	906	cell-specific	cell-specific	JJ
907	918	differences	difference	NNS
919	921	in	in	IN
922	937	transactivation	transactivation	NN
937	938	.	.	.

939	943	This	this	DT
944	952	sequence	sequence	NN
953	961	contains	contain	VBZ
962	971	consensus	consensus	NN
972	980	elements	element	NNS
981	991	consistent	consistent	JJ
992	996	with	with	IN
997	1013	myeloid-specific	myeloid-specific	JJ
1014	1018	gene	gene	NN
1019	1029	expression	expression	NN
1029	1030	,	,	,
1031	1040	including	include	VBG
1041	1042	a	a	DT
1043	1047	PU.1	pu.1	NN
1048	1057	consensus	consensus	NN
1058	1062	site	site	NN
1063	1067	near	near	IN
1068	1071	the	the	DT
1072	1077	major	major	JJ
1078	1091	transcription	transcription	NN
1092	1097	start	start	NN
1098	1102	site	site	NN
1103	1106	and	and	CC
1107	1108	a	a	DT
1109	1116	cluster	cluster	NN
1117	1119	of	of	IN
1120	1125	c-Myb	c-myb	NN
1126	1131	sites	site	NNS
1132	1139	located	located	JJ
1140	1147	several	several	JJ
1148	1155	hundred	hundred	CD
1156	1161	bases	base	NNS
1162	1170	upstream	upstream	RB
1171	1173	of	of	IN
1174	1178	this	this	DT
1179	1185	region	region	NN
1185	1186	.	.	.

1187	1194	However	however	RB
1194	1195	,	,	,
1196	1204	analysis	analysis	NN
1205	1207	of	of	IN
1208	1216	deletion	deletion	NN
1217	1224	mutants	mutant	NNS
1225	1234	localized	localize	VBD
1235	1241	nearly	nearly	RB
1242	1245	all	all	DT
1246	1248	of	of	IN
1249	1252	the	the	DT
1253	1261	promoter	promoter	NN
1262	1270	activity	activity	NN
1271	1273	to	to	TO
1274	1275	a	a	DT
1276	1281	short	short	JJ
1282	1288	region	region	NN
1289	1290	(	(	(
1290	1293	-73	-73	CD
1294	1296	to	to	TO
1297	1300	-16	-16	CD
1300	1301	)	)	)
1302	1306	that	that	WDT
1307	1310	did	do	VBD
1311	1314	not	not	RB
1315	1322	include	include	VB
1323	1326	the	the	DT
1327	1334	cluster	cluster	NN
1335	1337	of	of	IN
1338	1343	c-Myb	c-myb	NN
1344	1349	sites	site	NNS
1349	1350	.	.	.

1351	1352	A	a	DT
1353	1357	4-bp	4-bp	JJ
1358	1366	mutation	mutation	NN
1367	1369	of	of	IN
1370	1373	the	the	DT
1374	1378	core	core	NN
1379	1382	Sp1	sp1	NN
1383	1392	consensus	consensus	NN
1393	1400	element	element	NN
1401	1402	(	(	(
1402	1404	GC	GC	NNP
1405	1408	box	box	NN
1408	1409	)	)	)
1410	1411	(	(	(
1411	1414	-20	-20	CD
1414	1415	)	)	)
1416	1423	reduced	reduce	VBD
1424	1431	overall	overall	JJ
1432	1440	promoter	promoter	NN
1441	1449	activity	activity	NN
1450	1452	of	of	IN
1453	1456	the	the	DT
1457	1461	1-kb	1-kb	JJ
1462	1470	fragment	fragment	NN
1470	1471	.	.	.

1472	1480	Mutation	mutation	NN
1481	1483	of	of	IN
1484	1487	the	the	DT
1488	1492	PU.1	pu.1	NN
1493	1497	site	site	NN
1498	1501	did	do	VBD
1502	1505	not	not	RB
1506	1519	significantly	significantly	RB
1520	1526	affect	affect	VB
1527	1535	promoter	promoter	NN
1536	1544	activity	activity	NN
1544	1545	.	.	.

1546	1550	Only	only	RB
1551	1552	a	a	DT
1553	1558	small	small	JJ
1559	1565	region	region	NN
1566	1567	(	(	(
1567	1570	-35	-35	CD
1571	1573	to	to	TO
1574	1577	+22	+22	CD
1577	1578	)	)	)
1579	1588	including	include	VBG
1589	1592	the	the	DT
1593	1596	Sp1	sp1	NN
1597	1604	element	element	NN
1605	1608	and	and	CC
1609	1622	transcription	transcription	NN
1623	1628	start	start	NN
1629	1633	site	site	NN
1633	1634	,	,	,
1635	1638	but	but	CC
1639	1642	not	not	RB
1643	1646	the	the	DT
1647	1651	PU.1	pu.1	NN
1652	1656	site	site	NN
1657	1660	was	be	VBD
1661	1672	footprinted	footprinte	VBN
1672	1673	.	.	.

1674	1677	The	the	DT
1678	1682	4-bp	4-bp	JJ
1683	1691	mutation	mutation	NN
1692	1694	of	of	IN
1695	1698	the	the	DT
1699	1703	core	core	NN
1704	1707	Sp1	sp1	NN
1708	1717	consensus	consensus	NN
1718	1725	element	element	NN
1726	1735	abolished	abolish	VBD
1736	1748	footprinting	footprinting	NN
1749	1751	at	at	IN
1752	1755	the	the	DT
1756	1760	site	site	NN
1761	1764	and	and	CC
1765	1767	an	an	DT
1768	1776	antibody	antibody	NN
1777	1788	super-shift	super-shift	JJ
1789	1797	reaction	reaction	NN
1798	1804	showed	show	VBD
1805	1809	that	that	IN
1810	1813	Sp1	sp1	NN
1814	1816	is	be	VBZ
1817	1820	one	one	CD
1821	1823	of	of	IN
1824	1827	the	the	DT
1828	1835	factors	factor	NNS
1836	1843	binding	bind	VBG
1844	1847	the	the	DT
1848	1857	consensus	consensus	NN
1858	1862	site	site	NN
1862	1863	.	.	.

1864	1867	The	the	DT
1868	1871	Sp1	sp1	NN
1872	1876	site	site	NN
1877	1881	also	also	RB
1882	1894	co-localizes	co-localize	VBZ
1895	1899	with	with	IN
1900	1901	a	a	DT
1902	1907	DNase	DNase	NNP
1908	1909	I	i	CD
1910	1924	hypersensitive	hypersensitive	JJ
1925	1929	site	site	NN
1929	1930	.	.	.

1931	1934	The	the	DT
1935	1942	results	result	NNS
1943	1951	indicate	indicate	VBP
1952	1956	that	that	IN
1957	1960	DNA	dna	NN
1961	1972	methylation	methylation	NN
1972	1973	,	,	,
1974	1983	chromatin	chromatin	NN
1984	1993	structure	structure	NN
1993	1994	,	,	,
1995	1998	and	and	CC
1999	2014	transactivation	transactivation	NN
2015	2017	at	at	IN
2018	2020	an	an	DT
2021	2024	Sp1	sp1	NN
2025	2029	site	site	NN
2030	2040	contribute	contribute	VBP
2041	2043	to	to	TO
2044	2047	the	the	DT
2048	2054	highly	highly	RB
2055	2065	restricted	restricted	JJ
2066	2076	expression	expression	NN
2077	2079	of	of	IN
2080	2084	this	this	DT
2085	2099	myelomonocytic	myelomonocytic	JJ
2100	2107	lineage	lineage	NN
2108	2116	specific	specific	JJ
2117	2121	gene	gene	NN
2121	2122	.	.	.