0 15 Transcriptional transcriptional JJ 16 24 activity activity NN 25 28 and and CC 29 41 constitutive constitutive JJ 42 49 nuclear nuclear JJ 50 62 localization localization NN 63 65 of of IN 66 69 the the DT 70 73 ETS ETS NNP 74 81 protein protein NN 82 87 Elf-1 elf-1 NN 87 88 . . . 90 95 Elf-1 Elf-1 NNP 96 98 is be VBZ 99 100 a a DT 101 118 lymphoid-specific lymphoid-specific JJ 119 132 transcription transcription NN 133 139 factor factor NN 140 144 that that WDT 145 152 belongs belong VBZ 153 155 to to TO 156 159 the the DT 160 163 ETS ETS NNP 164 171 protein protein NN 172 178 family family NN 178 179 . . . 180 182 It it PRP 183 186 can can MD 187 191 bind bind VB 192 194 to to TO 195 198 DNA DNA NNP 199 205 target target NN 206 215 sequences sequence NNS 216 222 within within IN 223 224 a a DT 225 232 variety variety NN 233 235 of of IN 236 244 cytokine cytokine NN 245 250 genes gene NNS 250 251 . . . 252 254 We we PRP 255 266 demonstrate demonstrate VBP 267 271 that that IN 272 277 Elf-1 elf-1 NN 278 280 is be VBZ 281 295 constitutively constitutively RB 296 305 localized localize VBN 306 308 in in IN 309 312 the the DT 313 320 nucleus nucleus NN 321 326 which which WDT 327 329 is be VBZ 330 339 dependent dependent JJ 340 342 on on IN 343 346 the the DT 347 355 presence presence NN 356 358 of of IN 359 364 amino amino NN 365 370 acids acid NNS 371 377 86-265 86-265 CD 377 378 . . . 379 387 Analysis Analysis NNP 388 390 of of IN 391 401 Gal4-Elf-1 gal4-elf-1 NN 402 408 fusion fusion NN 409 417 proteins protein NNS 418 426 revealed reveal VBD 427 431 that that IN 432 435 the the DT 436 446 N-terminal n-terminal JJ 447 449 86 86 CD 450 455 amino amino NN 456 461 acids acid NNS 462 464 of of IN 465 470 Elf-1 elf-1 NN 471 478 contain contain VBP 479 480 a a DT 481 496 transcriptional transcriptional JJ 497 507 activation activation NN 508 514 domain domain NN 514 515 , , , 516 519 the the DT 520 528 activity activity NN 529 531 of of IN 532 537 which which WDT 538 540 is be VBZ 541 551 attenuated attenuate VBN 552 554 by by IN 555 557 an an DT 558 566 internal internal JJ 567 577 repression repression NN 578 584 domain domain NN 584 585 . . . 586 597 Furthermore furthermore RB 597 598 , , , 599 604 Elf-1 Elf-1 NNP 605 614 interacts interact VBZ 615 627 specifically specifically RB 628 632 with with IN 633 636 the the DT 637 640 E74 e74 NN 641 647 target target NN 648 656 sequence sequence NN 657 660 and and CC 661 664 can can MD 665 674 stimulate stimulate VB 675 688 transcription transcription NN 689 695 driven drive VBN 696 698 by by IN 699 702 the the DT 703 706 E74 e74 NN 707 711 site site NN 712 723 independent independent JJ 724 726 of of IN 727 736 mitogenic mitogenic JJ 737 746 signaling signaling NN 746 747 . . . 748 752 Thus thus RB 752 753 , , , 754 759 Elf-1 Elf-1 NNP 760 762 is be VBZ 763 767 able able JJ 768 770 to to TO 771 780 stimulate stimulate VB 781 785 gene gene NN 786 799 transcription transcription NN 800 805 which which WDT 806 809 may may MD 810 812 be be VB 813 821 required require VBN 822 825 for for IN 826 829 the the DT 830 841 development development NN 842 845 and and CC 846 854 activity activity NN 855 857 of of IN 858 869 lymphocytes lymphocyte NNS 869 870 . . .