0 5 Human human JJ 6 21 cytomegalovirus cytomegalovirus NN 22 29 induces induce VBZ 30 43 interleukin-8 interleukin-8 NN 44 54 production production NN 55 57 by by IN 58 59 a a DT 60 65 human human JJ 66 75 monocytic monocytic JJ 76 80 cell cell NN 81 85 line line NN 85 86 , , , 87 92 THP-1 thp-1 NN 92 93 , , , 94 101 through through IN 102 108 acting act VBG 109 121 concurrently concurrently RB 122 124 on on IN 125 130 AP-1- ap-1- NN 131 134 and and CC 135 152 NF-kappaB-binding nf-kappab-binding JJ 153 158 sites site NNS 159 161 of of IN 162 165 the the DT 166 179 interleukin-8 interleukin-8 NN 180 184 gene gene NN 184 185 . . . 187 202 Cytomegalovirus Cytomegalovirus NNP 203 204 ( ( ( 204 207 CMV CMV NNP 207 208 ) ) ) 209 218 infection infection NN 219 226 induced induce VBD 227 240 interleukin-8 interleukin-8 NN 241 242 ( ( ( 242 246 IL-8 il-8 NN 246 247 ) ) ) 248 252 gene gene NN 253 266 transcription transcription NN 267 269 in in IN 270 271 a a DT 272 277 human human JJ 278 287 monocytic monocytic JJ 288 292 cell cell NN 293 297 line line NN 297 298 , , , 299 304 THP-1 thp-1 NN 305 310 cells cell NNS 310 311 , , , 312 319 leading lead VBG 320 322 to to TO 323 327 IL-8 il-8 NN 328 337 secretion secretion NN 337 338 . . . 339 342 The the DT 343 353 functional functional JJ 354 362 analysis analysis NN 363 365 of of IN 366 369 the the DT 370 374 IL-8 il-8 NN 375 379 gene gene NN 380 388 revealed reveal VBD 389 393 that that IN 394 398 both both CC 399 404 AP-1- AP-1- NNP 405 408 and and CC 409 418 NF-kappaB NF-kappaB NNP 419 433 factor-binding factor-binding JJ 434 442 elements element NNS 443 447 were be VBD 448 456 involved involve VBN 457 459 in in IN 460 470 conferring confer VBG 471 474 the the DT 475 489 responsiveness responsiveness NN 490 492 to to TO 493 496 CMV CMV NNP 496 497 . . . 498 506 Moreover moreover RB 506 507 , , , 508 523 electrophoretic electrophoretic JJ 524 532 mobility mobility NN 533 538 shift shift NN 539 545 assays assay NNS 546 558 demonstrated demonstrate VBD 559 563 that that IN 564 567 CMV CMV NNP 568 575 induced induce VBD 576 579 the the DT 580 589 formation formation NN 590 592 of of IN 593 602 NF-kappaB NF-kappaB NNP 603 606 and and CC 607 611 AP-1 ap-1 NN 612 621 complexes complex NNS 621 622 . . . 623 628 These these DT 629 636 results result NNS 637 644 suggest suggest VBP 645 649 that that IN 650 653 CMV CMV NNP 654 663 activates activate VBZ 664 669 these these DT 670 685 transcriptional transcriptional JJ 686 693 factors factor NNS 693 694 , , , 695 704 resulting result VBG 705 707 in in IN 708 712 IL-8 il-8 NN 713 717 gene gene NN 718 728 expression expression NN 728 729 . . .