0 10 Comparison comparison NN 11 13 of of IN 14 17 the the DT 18 33 transactivation transactivation NN 34 41 domains domain NNS 42 44 of of IN 45 50 Stat5 Stat5 NNP 51 54 and and CC 55 60 Stat6 Stat6 NNP 61 63 in in IN 64 72 lymphoid lymphoid JJ 73 78 cells cell NNS 79 82 and and CC 83 90 mammary mammary JJ 91 101 epithelial epithelial JJ 102 107 cells cell NNS 107 108 . . . 110 114 Stat stat NN 115 116 ( ( ( 116 122 signal signal NN 123 134 transducers transducer NNS 135 138 and and CC 139 149 activators activator NNS 150 152 of of IN 153 166 transcription transcription NN 166 167 ) ) ) 168 171 and and CC 172 175 Jak Jak NNP 176 177 ( ( ( 177 182 Janus Janus NNP 183 190 kinases kinase NNS 190 191 ) ) ) 192 200 proteins protein NNS 201 204 are be VBP 205 212 central central JJ 213 223 components component NNS 224 226 in in IN 227 230 the the DT 231 237 signal signal NN 238 250 transduction transduction NN 251 257 events event NNS 258 260 in in IN 261 274 hematopoietic hematopoietic JJ 275 278 and and CC 279 289 epithelial epithelial JJ 290 295 cells cell NNS 295 296 . . . 297 301 They they PRP 302 305 are be VBP 306 313 rapidly rapidly RB 314 323 activated activate VBN 324 326 by by IN 327 334 various various JJ 335 344 cytokines cytokine NNS 344 345 , , , 346 354 hormones hormone NNS 354 355 , , , 356 359 and and CC 360 366 growth growth NN 367 374 factors factor NNS 374 375 . . . 376 380 Upon upon IN 381 387 ligand ligand NN 388 395 binding binding NN 396 399 and and CC 400 408 cytokine cytokine NN 409 417 receptor receptor NN 418 430 dimerization dimerization NN 430 431 , , , 432 436 Stat Stat NNP 437 445 proteins protein NNS 446 449 are be VBP 450 464 phosphorylated phosphorylate VBN 465 467 on on IN 468 476 tyrosine tyrosine NN 477 485 residues residue NNS 486 488 by by IN 489 492 Jak Jak NNP 493 500 kinases kinase NNS 500 501 . . . 502 511 Activated activate VBN 512 516 Stat stat NN 517 525 proteins protein NNS 526 530 form form VBP 531 536 homo- homo- JJ 537 539 or or CC 540 552 heterodimers heterodimer NNS 552 553 , , , 554 565 translocate translocate VBP 566 568 to to TO 569 572 the the DT 573 580 nucleus nucleus NN 580 581 , , , 582 585 and and CC 586 592 induce induce VBP 593 606 transcription transcription NN 607 611 from from IN 612 622 responsive responsive JJ 623 628 genes gene NNS 628 629 . . . 630 635 Stat5 Stat5 NNP 636 639 and and CC 640 645 Stat6 Stat6 NNP 646 649 are be VBP 650 663 transcription transcription NN 664 671 factors factor NNS 672 678 active active JJ 679 681 in in IN 682 689 mammary mammary JJ 690 700 epithelial epithelial JJ 701 706 cells cell NNS 707 710 and and CC 711 717 immune immune JJ 718 723 cells cell NNS 723 724 . . . 725 734 Prolactin Prolactin NNP 735 744 activates activate VBZ 745 750 Stat5 stat5 NN 750 751 , , , 752 755 and and CC 756 769 interleukin-4 interleukin-4 NN 770 771 ( ( ( 771 775 IL-4 il-4 NN 775 776 ) ) ) 777 786 activates activate VBZ 787 792 Stat6 Stat6 NNP 792 793 . . . 794 798 Both both DT 799 808 cytokines cytokine NNS 809 812 are be VBP 813 817 able able JJ 818 820 to to TO 821 830 stimulate stimulate VB 831 835 cell cell NN 836 849 proliferation proliferation NN 849 850 , , , 851 866 differentiation differentiation NN 866 867 , , , 868 871 and and CC 872 880 survival survival NN 880 881 . . . 882 884 We we PRP 885 897 investigated investigate VBD 898 901 the the DT 902 917 transactivation transactivation NN 918 927 potential potential NN 928 930 of of IN 931 936 Stat6 Stat6 NNP 937 940 and and CC 941 946 found find VBD 947 951 that that IN 952 954 it it PRP 955 957 is be VBZ 958 961 not not RB 962 972 restricted restrict VBN 973 975 to to TO 976 987 lymphocytes lymphocyte NNS 987 988 . . . 989 1003 IL-4-dependent il-4-dependent JJ 1004 1014 activation activation NN 1015 1017 of of IN 1018 1023 Stat6 Stat6 NNP 1024 1027 was be VBD 1028 1032 also also RB 1033 1041 observed observe VBN 1042 1044 in in IN 1045 1049 HC11 hc11 NN 1050 1057 mammary mammary JJ 1058 1068 epithelial epithelial JJ 1069 1074 cells cell NNS 1074 1075 . . . 1076 1078 In in IN 1079 1084 these these DT 1085 1090 cells cell NNS 1090 1091 , , , 1092 1097 Stat6 Stat6 NNP 1098 1108 activation activation NN 1109 1112 led lead VBD 1113 1115 to to TO 1116 1119 the the DT 1120 1129 induction induction NN 1130 1132 of of IN 1133 1136 the the DT 1137 1148 beta-casein beta-casein NN 1149 1153 gene gene NN 1154 1162 promoter promoter NN 1162 1163 . . . 1164 1167 The the DT 1168 1177 induction induction NN 1178 1180 of of IN 1181 1185 this this DT 1186 1194 promoter promoter NN 1195 1198 was be VBD 1199 1208 confirmed confirm VBN 1209 1211 in in IN 1212 1216 COS7 cos7 NN 1217 1222 cells cell NNS 1222 1223 . . . 1224 1227 The the DT 1228 1242 glucocorticoid glucocorticoid NN 1243 1251 receptor receptor NN 1252 1255 was be VBD 1256 1260 able able JJ 1261 1263 to to TO 1264 1271 further further RB 1272 1279 enhance enhance VB 1280 1292 IL-4-induced il-4-induced JJ 1293 1297 gene gene NN 1298 1311 transcription transcription NN 1312 1319 through through IN 1320 1323 the the DT 1324 1330 action action NN 1331 1333 of of IN 1334 1339 Stat6 Stat6 NNP 1339 1340 . . . 1341 1349 Deletion deletion NN 1350 1358 analysis analysis NN 1359 1361 of of IN 1362 1365 the the DT 1366 1383 carboxyl-terminal carboxyl-terminal JJ 1384 1390 region region NN 1391 1393 of of IN 1394 1399 Stat6 Stat6 NNP 1400 1403 and and CC 1404 1417 recombination recombination NN 1418 1420 of of IN 1421 1425 this this DT 1426 1432 region region NN 1433 1437 with with IN 1438 1439 a a DT 1440 1452 heterologous heterologous JJ 1453 1456 DNA dna NN 1457 1464 binding binding NN 1465 1471 domain domain NN 1472 1479 allowed allow VBD 1480 1483 the the DT 1484 1496 delimitation delimitation NN 1497 1500 and and CC 1501 1517 characterization characterization NN 1518 1520 of of IN 1521 1524 the the DT 1525 1540 transactivation transactivation NN 1541 1547 domain domain NN 1548 1550 of of IN 1551 1556 Stat6 Stat6 NNP 1556 1557 . . . 1558 1561 The the DT 1562 1571 potencies potency NNS 1572 1574 of of IN 1575 1578 the the DT 1579 1594 transactivation transactivation NN 1595 1602 domains domain NNS 1603 1605 of of IN 1606 1611 Stat5 Stat5 NNP 1611 1612 , , , 1613 1618 Stat6 Stat6 NNP 1618 1619 , , , 1620 1623 and and CC 1624 1629 viral viral JJ 1630 1637 protein protein NN 1638 1642 VP16 vp16 NN 1643 1647 were be VBD 1648 1656 compared compare VBN 1656 1657 . . . 1658 1663 Stat6 Stat6 NNP 1664 1667 had have VBD 1668 1669 a a DT 1670 1685 transactivation transactivation NN 1686 1692 domain domain NN 1693 1698 which which WDT 1699 1702 was be VBD 1703 1708 about about IN 1709 1716 10-fold 10-fold RB 1717 1725 stronger strong JJR 1726 1730 than than IN 1731 1735 that that DT 1736 1738 of of IN 1739 1744 Stat5 Stat5 NNP 1744 1745 . . . 1746 1748 In in IN 1749 1754 pre-B pre-b JJ 1755 1760 cells cell NNS 1761 1762 ( ( ( 1762 1767 Ba/F3 Ba/F3 NNP 1767 1768 ) ) ) 1768 1769 , , , 1770 1773 the the DT 1774 1789 transactivation transactivation NN 1790 1796 domain domain NN 1797 1799 of of IN 1800 1805 Stat6 Stat6 NNP 1806 1809 was be VBD 1810 1814 IL-4 il-4 NN 1815 1824 regulated regulated JJ 1824 1825 , , , 1826 1839 independently independently RB 1840 1844 from from IN 1845 1848 its its PRP$ 1849 1852 DNA dna NN 1853 1860 binding binding NN 1861 1869 function function NN 1869 1870 . . .