0 7 S-allyl s-allyl JJ 8 16 cysteine cysteine NN 17 25 inhibits inhibit VBZ 26 36 activation activation NN 37 39 of of IN 40 47 nuclear nuclear JJ 48 54 factor factor NN 55 60 kappa kappa NN 61 62 B b NN 63 65 in in IN 66 71 human human JJ 72 73 T t NN 74 79 cells cell NNS 79 80 . . . 82 90 Reactive reactive JJ 91 97 oxygen oxygen NN 98 105 species specie NNS 106 109 are be VBP 110 118 involved involve VBN 119 121 in in IN 122 128 signal signal NN 129 141 transduction transduction NN 142 150 pathways pathway NNS 151 158 leading lead VBG 159 161 to to TO 162 169 nuclear nuclear JJ 170 176 factor factor NN 177 182 kappa kappa NN 183 184 B B NNP 185 186 ( ( ( 186 194 NF-kappa NF-kappa NNP 195 196 B B NNP 196 197 ) ) ) 198 208 activation activation NN 209 214 which which WDT 215 218 has have VBZ 219 223 been be VBN 224 234 implicated implicate VBN 235 237 in in IN 238 241 the the DT 242 252 regulation regulation NN 253 255 of of IN 256 260 gene gene NN 261 274 transcription transcription NN 274 275 . . . 276 278 We we PRP 279 287 recently recently RB 288 296 reported report VBD 297 301 that that IN 302 303 a a DT 304 310 garlic garlic NN 311 319 compound compound NN 319 320 , , , 321 328 S-allyl s-allyl JJ 329 337 cysteine cysteine NN 338 339 ( ( ( 339 342 SAC SAC NNP 342 343 ) ) ) 343 344 , , , 345 353 protects protect VBZ 354 360 bovine bovine JJ 361 370 pulmonary pulmonary JJ 371 377 artery artery NN 378 389 endothelial endothelial JJ 390 395 cells cell NNS 396 400 from from IN 401 408 oxidant oxidant JJ 409 415 injury injury NN 416 423 induced induce VBN 424 426 by by IN 427 435 hydrogen hydrogen NN 436 444 peroxide peroxide NN 445 446 ( ( ( 446 450 H2O2 h2o2 NN 450 451 ) ) ) 451 452 . . . 453 455 In in IN 456 460 this this DT 461 466 study study NN 467 469 we we PRP 470 480 determined determine VBD 481 484 the the DT 485 492 effects effect NNS 493 495 of of IN 496 499 SAC SAC NNP 500 502 on on IN 503 511 NF-kappa NF-kappa NNP 512 513 B B NNP 514 524 activation activation NN 525 527 in in IN 528 533 human human JJ 534 535 T t NN 536 547 lymphocytes lymphocyte NNS 548 549 ( ( ( 549 555 Jurkat Jurkat NNP 556 561 cells cell NNS 561 562 ) ) ) 563 570 induced induce VBN 571 573 by by IN 574 579 tumor tumor NN 580 588 necrosis necrosis NN 589 595 factor factor NN 596 601 alpha alpha NN 602 603 ( ( ( 603 607 TNF- tnf- NN 608 613 alpha alpha NN 613 614 ) ) ) 615 618 and and CC 619 623 H2O2 H2O2 NNP 623 624 . . . 625 634 Activated activate VBN 635 643 NF-kappa NF-kappa NNP 644 645 B B NNP 646 648 in in IN 649 656 nuclear nuclear JJ 657 665 extracts extract NNS 666 669 was be VBD 670 678 measured measure VBN 679 681 by by IN 682 684 an an DT 685 700 electrophoretic electrophoretic JJ 701 709 mobility mobility NN 710 715 shift shift NN 716 721 assay assay NN 722 727 using use VBG 728 739 32P-labeled 32p-labeled JJ 740 745 probe probe NN 745 746 . . . 747 750 SAC sac NN 751 763 consistently consistently RB 764 773 exhibited exhibit VBD 774 775 a a DT 776 790 dose-dependent dose-dependent JJ 791 801 inhibition inhibition NN 802 804 of of IN 805 813 NF-kappa NF-kappa NNP 814 815 B B NNP 816 826 activation activation NN 827 834 induced induce VBN 835 837 by by IN 838 842 both both CC 843 852 TNF-alpha TNF-alpha NNP 853 856 and and CC 857 861 H2O2 H2O2 NNP 861 862 . . . 863 873 Supershift Supershift NNP 874 878 with with IN 879 887 specific specific JJ 888 898 antibodies antibody NNS 899 901 to to TO 902 910 NF-kappa NF-kappa NNP 911 912 B B NNP 913 921 subunits subunit NNS 922 931 confirmed confirm VBD 932 936 that that IN 937 940 the the DT 941 950 inducible inducible JJ 951 959 retarded retard VBN 960 965 bands band NNS 966 974 observed observe VBN 975 977 in in IN 978 981 the the DT 982 986 EMSA EMSA NNP 987 990 and and CC 991 998 p65-p50 p65-p50 NN 999 1010 heterodimer heterodimer NN 1011 1013 of of IN 1014 1017 the the DT 1018 1026 NF-kappa NF-kappa NNP 1027 1032 B/Rel B/Rel NNP 1033 1040 protein protein NN 1040 1041 . . . 1042 1045 Our our PRP$ 1046 1050 data datum NNS 1051 1058 suggest suggest VBP 1059 1063 that that IN 1064 1067 SAC SAC NNP 1068 1071 may may MD 1072 1075 act act VB 1076 1079 via via IN 1080 1091 antioxidant antioxidant JJ 1092 1102 mechanisms mechanism NNS 1103 1105 to to TO 1106 1111 block block VB 1112 1120 NF-kappa NF-kappa NNP 1121 1122 B B NNP 1123 1133 activation activation NN 1134 1136 in in IN 1137 1143 Jurkat Jurkat NNP 1144 1149 cells cell NNS 1149 1150 . . .