0 6 Effect effect NN 7 9 of of IN 10 20 adenovirus adenovirus NN 21 22 2 2 CD 23 25 on on IN 26 34 cellular cellular JJ 35 39 gene gene NN 40 50 activation activation NN 51 53 in in IN 54 67 blood-derived blood-derived JJ 68 77 monocytes monocyte NNS 78 81 and and CC 82 93 macrophages macrophage NNS 93 94 . . . 96 98 We we PRP 99 103 have have VBP 104 116 investigated investigate VBN 117 120 the the DT 121 127 effect effect NN 128 130 of of IN 131 141 adenovirus adenovirus NN 142 143 2 2 CD 144 145 ( ( ( 145 148 Ad2 ad2 NN 148 149 ) ) ) 150 159 infection infection NN 160 162 on on IN 163 168 human human JJ 169 178 monocytes monocyte NNS 179 182 and and CC 183 199 monocyte-derived monocyte-derived JJ 200 211 macrophages macrophage NNS 212 216 with with IN 217 223 regard regard NN 224 226 to to TO 227 237 expression expression NN 238 240 of of IN 241 250 TNF-alpha TNF-alpha NNP 251 254 and and CC 255 259 IL-1 il-1 NN 260 264 beta beta NN 264 265 . . . 266 268 In in IN 269 278 monocytes monocyte NNS 278 279 , , , 280 283 the the DT 284 289 virus virus NN 290 293 was be VBD 294 299 bound bind VBN 300 302 to to TO 303 306 the the DT 307 314 surface surface NN 315 322 without without IN 323 328 being be VBG 329 341 internalized internalize VBN 341 342 . . . 343 345 On on IN 346 349 the the DT 350 355 other other JJ 356 360 hand hand NN 360 361 , , , 362 365 Ad2 Ad2 NNP 366 369 was be VBD 370 382 internalized internalize VBN 383 385 by by IN 386 397 macrophages macrophage NNS 397 398 . . . 399 401 No no DT 402 407 virus virus NN 408 419 replication replication NN 420 423 and and CC 424 426 no no DT 427 440 transcription transcription NN 441 443 of of IN 444 447 the the DT 448 451 Ad2 ad2 NN 452 457 early early JJ 458 463 genes gene NNS 464 467 was be VBD 468 476 observed observe VBN 477 479 in in IN 480 486 either either DT 487 489 of of IN 490 493 the the DT 494 499 cells cell NNS 499 500 . . . 501 504 Ad2 ad2 NN 505 514 infection infection NN 515 522 induced induce VBD 523 532 transient transient JJ 533 541 increase increase NN 542 544 in in IN 545 548 the the DT 549 553 mRNA mrna NN 554 560 levels level NNS 561 564 for for IN 565 574 TNF-alpha TNF-alpha NNP 575 578 and and CC 579 583 IL-1 il-1 NN 584 588 beta beta NN 589 591 in in IN 592 596 both both CC 597 606 monocytes monocyte NNS 607 610 and and CC 611 613 in in IN 614 625 macrophages macrophage NNS 625 626 , , , 627 635 although although IN 636 639 the the DT 640 648 kinetics kinetic NNS 649 651 of of IN 652 655 the the DT 656 669 transcription transcription NN 670 673 was be VBD 674 682 slightly slightly RB 683 692 different different JJ 692 693 . . . 694 697 The the DT 698 708 production production NN 709 711 of of IN 712 716 both both DT 717 726 cytokines cytokine NNS 726 727 , , , 728 736 measured measure VBN 737 739 by by IN 740 745 ELISA ELISA NNP 746 751 tests test NNS 751 752 , , , 753 756 was be VBD 757 765 enhanced enhance VBN 766 768 in in IN 769 778 monocytes monocyte NNS 778 779 . . . 780 782 In in IN 783 794 macrophages macrophage NNS 794 795 , , , 796 797 a a DT 798 804 slight slight JJ 805 816 enhancement enhancement NN 817 819 of of IN 820 829 TNF-alpha TNF-alpha NNP 830 840 production production NN 841 844 was be VBD 845 849 seen see VBN 849 850 , , , 851 858 whereas whereas IN 859 863 IL-1 il-1 NN 864 868 beta beta NN 869 872 was be VBD 873 876 not not RB 877 885 detected detect VBN 885 886 . . . 887 890 The the DT 891 895 data datum NNS 896 904 indicate indicate VBP 905 909 that that IN 910 918 cellular cellular JJ 919 924 genes gene NNS 925 930 might might MD 931 933 be be VB 934 943 activated activate VBN 944 946 by by IN 947 950 Ad2 ad2 NN 951 956 virus virus NN 957 966 infection infection NN 967 969 in in IN 970 983 nonpermissive nonpermissive JJ 984 989 cells cell NNS 990 995 where where WRB 996 998 no no DT 999 1004 viral viral JJ 1005 1009 gene gene NN 1010 1018 products product NNS 1019 1024 could could MD 1025 1027 be be VB 1028 1036 detected detect VBN 1036 1037 . . .