0 4 AP-1 ap-1 NN 5 12 derived derive VBN 13 17 from from IN 18 24 mature mature JJ 25 34 monocytes monocyte NNS 35 38 and and CC 39 49 astrocytes astrocyte NNS 50 64 preferentially preferentially RB 65 74 interacts interact VBZ 75 79 with with IN 80 83 the the DT 84 90 HTLV-I HTLV-I NNP 91 99 promoter promoter NN 100 107 central central JJ 108 110 21 21 CD 111 113 bp bp NN 114 120 repeat repeat NN 120 121 . . . 123 139 Characterization characterization NN 140 142 of of IN 143 146 the the DT 147 155 cellular cellular JJ 156 169 transcription transcription NN 170 177 factors factor NNS 178 189 interacting interact VBG 190 194 with with IN 195 198 the the DT 199 204 human human JJ 205 206 T t NN 207 211 cell cell NN 212 224 lymphotropic lymphotropic JJ 225 230 virus virus NN 231 235 type type NN 236 237 I i CD 238 239 ( ( ( 239 245 HTLV-I HTLV-I NNP 245 246 ) ) ) 247 251 long long JJ 252 260 terminal terminal JJ 261 267 repeat repeat NN 268 269 ( ( ( 269 272 LTR LTR NNP 272 273 ) ) ) 274 276 is be VBZ 277 286 essential essential JJ 287 289 to to TO 290 300 dissecting dissect VBG 301 304 the the DT 305 315 mechanisms mechanism NNS 316 324 involved involve VBN 325 327 in in IN 328 333 viral viral JJ 334 347 transcription transcription NN 348 352 that that WDT 353 356 may may MD 357 359 be be VB 360 369 pertinent pertinent JJ 370 372 to to TO 373 376 the the DT 377 386 oncogenic oncogenic JJ 387 390 and and CC 391 406 neuropathogenic neuropathogenic JJ 407 416 processes process NNS 417 427 associated associate VBN 428 432 with with IN 433 439 HTLV-I HTLV-I NNP 440 449 infection infection NN 450 452 in in IN 453 457 both both CC 458 461 the the DT 462 468 immune immune JJ 469 472 and and CC 473 480 nervous nervous JJ 481 488 systems system NNS 488 489 . . . 490 505 Electrophoretic electrophoretic JJ 506 514 mobility mobility NN 515 520 shift shift NN 521 522 ( ( ( 522 525 EMS EMS NNP 525 526 ) ) ) 527 535 analyses analysis NNS 536 545 utilizing utilize VBG 546 562 oligonucleotides oligonucleotide NNS 563 573 homologous homologous JJ 574 576 to to TO 577 581 each each DT 582 584 of of IN 585 588 the the DT 589 591 21 21 CD 592 594 bp bp NN 595 601 repeat repeat NN 602 610 elements element NNS 611 618 reacted react VBD 619 623 with with IN 624 631 nuclear nuclear JJ 632 640 extracts extract NNS 641 648 derived derive VBN 649 653 from from IN 654 658 cell cell NN 659 664 lines line NNS 665 667 of of IN 668 679 lymphocytic lymphocytic JJ 679 680 , , , 681 690 monocytic monocytic JJ 690 691 , , , 692 700 neuronal neuronal JJ 700 701 , , , 702 705 and and CC 706 711 glial glial JJ 712 716 cell cell NN 717 723 origin origin NN 724 728 have have VBP 729 741 demonstrated demonstrate VBN 742 754 differential differential JJ 755 762 binding binding NN 763 765 of of IN 766 774 cellular cellular JJ 775 782 factors factor NNS 783 785 to to TO 786 789 the the DT 790 795 three three CD 796 798 21 21 CD 799 801 bp bp NN 802 809 repeats repeat NNS 810 811 ( ( ( 811 814 1-4 1-4 CD 814 815 ) ) ) 815 816 . . . 817 825 ATF/CREB ATF/CREB NNP 826 829 and and CC 830 832 Sp Sp NNP 833 839 family family NN 840 847 members member NNS 848 858 interacted interact VBD 859 863 with with IN 864 867 the the DT 868 870 21 21 CD 871 873 bp bp NN 874 881 repeats repeat NNS 882 884 to to TO 885 889 form form VB 890 901 DNA-protein dna-protein JJ 902 911 complexes complex NNS 912 918 common common JJ 919 921 to to TO 922 925 all all DT 926 930 cell cell NN 931 936 types type NNS 937 945 examined examine VBN 945 946 . . . 947 954 However however RB 954 955 , , , 956 957 a a DT 958 964 unique unique JJ 965 976 DNA-protein dna-protein JJ 977 984 complex complex NN 985 988 was be VBD 989 997 detected detect VBN 998 1002 when when WRB 1003 1006 the the DT 1007 1015 promoter promoter NN 1016 1023 central central JJ 1024 1026 21 21 CD 1027 1029 bp bp NN 1030 1036 repeat repeat NN 1037 1040 was be VBD 1041 1048 reacted react VBN 1049 1053 with with IN 1054 1061 nuclear nuclear JJ 1062 1070 extracts extract NNS 1071 1078 derived derive VBN 1079 1083 from from IN 1084 1090 either either CC 1091 1094 the the DT 1095 1100 U-373 u-373 NN 1101 1103 MG MG NNP 1104 1116 glioblastoma glioblastoma NN 1117 1121 cell cell NN 1122 1126 line line NN 1127 1129 or or CC 1130 1133 the the DT 1134 1139 THP-1 thp-1 NN 1140 1146 mature mature JJ 1147 1156 monocytic monocytic JJ 1157 1161 cell cell NN 1162 1166 line line NN 1166 1167 . . . 1168 1173 Based base VBN 1174 1176 on on IN 1177 1187 nucleotide nucleotide NN 1188 1196 sequence sequence NN 1197 1209 requirements requirement NNS 1210 1213 and and CC 1214 1230 immunoreactivity immunoreactivity NN 1230 1231 , , , 1232 1234 we we PRP 1235 1246 demonstrate demonstrate VBP 1247 1251 that that IN 1252 1256 this this DT 1257 1268 DNA-protein dna-protein JJ 1269 1276 complex complex NN 1277 1279 is be VBZ 1280 1289 comprised comprise VBN 1290 1292 of of IN 1293 1296 the the DT 1297 1301 AP-1 ap-1 NN 1302 1312 components component NNS 1312 1313 , , , 1314 1317 Fos Fos NNP 1318 1321 and and CC 1322 1325 Jun Jun NNP 1325 1326 . . .