0	2	Of	of	IN
3	6	the	the	DT
7	19	GATA-binding	gata-binding	JJ
20	28	proteins	protein	NNS
28	29	,	,	,
30	34	only	only	RB
35	41	GATA-4	gata-4	NN
42	53	selectively	selectively	RB
54	63	regulates	regulate	VBZ
64	67	the	the	DT
68	73	human	human	JJ
74	78	IL-5	il-5	NN
79	83	gene	gene	NN
84	92	promoter	promoter	NN
93	95	in	in	IN
96	100	IL-5	il-5	NN
101	110	producing	producing	NN
111	116	cells	cell	NNS
117	122	which	which	WDT
123	130	express	express	VBP
131	139	multiple	multiple	JJ
140	152	GATA-binding	gata-binding	JJ
153	161	proteins	protein	NNS
161	162	.	.	.

164	177	Interleukin-5	Interleukin-5	NNP
178	179	(	(	(
179	183	IL-5	IL-5	NNP
183	184	)	)	)
185	187	is	be	VBZ
188	196	produced	produce	VBN
197	199	by	by	IN
200	201	T	t	NN
202	213	lymphocytes	lymphocyte	NNS
214	217	and	and	CC
218	223	known	know	VBN
224	226	to	to	TO
227	234	support	support	VB
235	236	B	B	NNP
237	241	cell	cell	NN
242	248	growth	growth	NN
249	252	and	and	CC
253	265	eosinophilic	eosinophilic	JJ
266	281	differentiation	differentiation	NN
282	284	of	of	IN
285	288	the	the	DT
289	299	progenitor	progenitor	NN
300	305	cells	cell	NNS
305	306	.	.	.

307	312	Using	use	VBG
313	320	ATL-16T	atl-16t	NN
321	326	cells	cell	NNS
327	332	which	which	WDT
333	340	express	express	VBP
341	345	IL-5	IL-5	NNP
346	350	mRNA	mRNA	NNP
350	351	,	,	,
352	354	we	we	PRP
355	359	have	have	VBP
360	370	identified	identify	VBN
371	372	a	a	DT
373	379	region	region	NN
379	380	,	,	,
381	387	within	within	IN
388	391	the	the	DT
392	397	human	human	JJ
398	402	IL-5	il-5	NN
403	407	gene	gene	NN
408	416	promoter	promoter	NN
416	417	,	,	,
418	422	that	that	WDT
423	432	regulates	regulate	VBZ
433	437	IL-5	IL-5	NNP
438	442	gene	gene	NN
443	456	transcription	transcription	NN
456	457	.	.	.

458	462	This	this	DT
463	473	cis-acting	cis-acting	JJ
474	482	sequence	sequence	NN
483	491	contains	contain	VBZ
492	495	the	the	DT
496	500	core	core	NN
501	508	binding	binding	NN
509	514	motif	motif	NN
514	515	,	,	,
516	517	(	(	(
517	530	A/T)GATA(A/G)	A/T)GATA(A/G)	NNP
530	531	,	,	,
532	535	for	for	IN
536	548	GATA-binding	gata-binding	JJ
549	555	family	family	NN
556	564	proteins	protein	NNS
565	568	and	and	CC
569	573	thus	thus	RB
574	582	suggests	suggest	VBZ
583	586	the	the	DT
587	598	involvement	involvement	NN
599	601	of	of	IN
602	607	these	these	DT
608	614	family	family	NN
615	622	members	member	NNS
622	623	.	.	.

624	626	In	in	IN
627	631	this	this	DT
632	638	report	report	NN
638	639	,	,	,
640	642	we	we	PRP
643	651	describe	describe	VBP
652	655	the	the	DT
656	663	cloning	cloning	NN
664	666	of	of	IN
667	672	human	human	JJ
673	679	GATA-4	gata-4	NN
680	681	(	(	(
681	688	hGATA-4	hgata-4	NN
688	689	)	)	)
690	693	and	and	CC
694	698	show	show	VBP
699	703	that	that	IN
704	711	hGATA-4	hgata-4	NN
712	723	selectively	selectively	RB
724	733	interacts	interact	VBZ
734	738	with	with	IN
739	742	the	the	DT
743	746	-70	-70	CD
747	751	GATA	GATA	NNP
752	756	site	site	NN
757	763	within	within	IN
764	767	the	the	DT
768	772	IL-5	IL-5	NNP
773	781	proximal	proximal	JJ
782	790	promoter	promoter	NN
791	797	region	region	NN
797	798	.	.	.

799	801	By	by	IN
802	810	promoter	promoter	NN
811	819	deletion	deletion	NN
820	823	and	and	CC
824	832	mutation	mutation	NN
833	841	analyses	analysis	NNS
841	842	,	,	,
843	845	we	we	PRP
846	857	established	establish	VBD
858	862	this	this	DT
863	869	region	region	NN
870	872	as	as	IN
873	874	a	a	DT
875	883	positive	positive	JJ
884	894	regulatory	regulatory	JJ
895	902	element	element	NN
902	903	.	.	.

904	918	Cotransfection	cotransfection	NN
919	930	experiments	experiment	NNS
931	939	revealed	reveal	VBD
940	944	that	that	IN
945	949	both	both	CC
950	957	hGATA-4	hgata-4	NN
958	961	and	and	CC
962	972	PMA/A23187	pma/a23187	NN
973	984	stimulation	stimulation	NN
985	988	are	be	VBP
989	998	necessary	necessary	JJ
999	1002	for	for	IN
1003	1006	the	the	DT
1007	1011	IL-5	IL-5	NNP
1012	1020	promoter	promoter	NN
1021	1031	activation	activation	NN
1031	1032	.	.	.

1033	1036	The	the	DT
1037	1048	requirement	requirement	NN
1049	1051	of	of	IN
1052	1059	another	another	DT
1060	1070	regulatory	regulatory	JJ
1071	1078	element	element	NN
1079	1085	called	call	VBN
1086	1090	CLE0	CLE0	NNP
1090	1091	,	,	,
1092	1097	which	which	WDT
1098	1102	lies	lie	VBZ
1103	1113	downstream	downstream	RB
1114	1116	of	of	IN
1117	1120	the	the	DT
1121	1124	-70	-70	CD
1125	1129	GATA	GATA	NNP
1130	1134	site	site	NN
1134	1135	,	,	,
1136	1139	was	be	VBD
1140	1144	also	also	RB
1145	1157	demonstrated	demonstrate	VBN
1157	1158	.	.	.

1159	1166	ATL-16T	ATL-16T	NNP
1167	1172	cells	cell	NNS
1173	1180	express	express	VBP
1181	1185	mRNA	mrna	NN
1186	1188	of	of	IN
1189	1194	three	three	CD
1195	1207	GATA-binding	gata-binding	JJ
1208	1216	proteins	protein	NNS
1216	1217	,	,	,
1218	1225	hGATA-2	hgata-2	NN
1225	1226	,	,	,
1227	1234	hGATA-3	hgata-3	NN
1235	1238	and	and	CC
1239	1246	hGATA-4	hgata-4	NN
1246	1247	,	,	,
1248	1251	and	and	CC
1252	1256	each	each	DT
1257	1259	of	of	IN
1260	1264	them	them	PRP
1265	1268	has	have	VBZ
1269	1270	a	a	DT
1271	1280	potential	potential	NN
1281	1283	to	to	TO
1284	1288	bind	bind	VB
1289	1291	to	to	TO
1292	1295	the	the	DT
1296	1305	consensus	consensus	NN
1306	1307	(	(	(
1307	1318	A/T)GATA(G/	A/T)GATA(G/	NNP
1319	1320	A	A	NNP
1320	1321	)	)	)
1322	1327	motif	motif	NN
1327	1328	.	.	.

1329	1336	However	however	RB
1336	1337	,	,	,
1338	1343	using	use	VBG
1344	1351	ATL-16T	ATL-16T	NNP
1352	1359	nuclear	nuclear	JJ
1360	1367	extract	extract	NN
1367	1368	,	,	,
1369	1371	we	we	PRP
1372	1384	demonstrated	demonstrate	VBD
1385	1389	that	that	IN
1390	1396	GATA-4	gata-4	NN
1397	1399	is	be	VBZ
1400	1403	the	the	DT
1404	1408	only	only	RB
1409	1421	GATA-binding	gata-binding	JJ
1422	1429	protein	protein	NN
1430	1434	that	that	WDT
1435	1440	forms	form	VBZ
1441	1449	specific	specific	JJ
1450	1461	DNA-protein	dna-protein	JJ
1462	1469	complex	complex	NN
1470	1474	with	with	IN
1475	1478	the	the	DT
1479	1482	-70	-70	CD
1483	1487	GATA	GATA	NNP
1488	1492	site	site	NN
1492	1493	.	.	.

1494	1497	The	the	DT
1498	1513	electrophoretic	electrophoretic	JJ
1514	1522	mobility	mobility	NN
1523	1528	shift	shift	NN
1529	1534	assay	assay	NN
1535	1539	with	with	IN
1540	1548	extracts	extract	NNS
1549	1551	of	of	IN
1552	1555	COS	COS	NNP
1556	1561	cells	cell	NNS
1562	1572	expressing	express	VBG
1573	1585	GATA-binding	gata-binding	JJ
1586	1594	proteins	protein	NNS
1595	1601	showed	show	VBD
1602	1606	that	that	IN
1607	1613	GATA-4	gata-4	NN
1614	1617	has	have	VBZ
1618	1621	the	the	DT
1622	1629	highest	high	JJS
1630	1637	binding	binding	NN
1638	1646	affinity	affinity	NN
1647	1649	to	to	TO
1650	1653	the	the	DT
1654	1657	-70	-70	CD
1658	1662	GATA	GATA	NNP
1663	1667	site	site	NN
1668	1673	among	among	IN
1674	1677	the	the	DT
1678	1683	three	three	CD
1684	1696	GATA-binding	gata-binding	JJ
1697	1705	proteins	protein	NNS
1705	1706	.	.	.

1707	1711	When	when	WRB
1712	1715	the	the	DT
1716	1731	transactivation	transactivation	NN
1732	1739	ability	ability	NN
1740	1743	was	be	VBD
1744	1752	compared	compare	VBN
1753	1758	among	among	IN
1759	1762	the	the	DT
1763	1768	three	three	CD
1768	1769	,	,	,
1770	1776	GATA-4	gata-4	NN
1777	1783	showed	show	VBD
1784	1787	the	the	DT
1788	1795	highest	high	JJS
1796	1804	activity	activity	NN
1804	1805	.	.	.

1806	1811	These	these	DT
1812	1819	results	result	NNS
1820	1831	demonstrate	demonstrate	VBP
1832	1835	the	the	DT
1836	1845	selective	selective	JJ
1846	1850	role	role	NN
1851	1853	of	of	IN
1854	1860	GATA-4	gata-4	NN
1861	1863	in	in	IN
1864	1867	the	the	DT
1868	1883	transcriptional	transcriptional	JJ
1884	1894	regulation	regulation	NN
1895	1897	of	of	IN
1898	1901	the	the	DT
1902	1906	IL-5	il-5	NN
1907	1911	gene	gene	NN
1912	1914	in	in	IN
1915	1916	a	a	DT
1917	1929	circumstance	circumstance	NN
1930	1935	where	where	WRB
1936	1944	multiple	multiple	JJ
1945	1952	members	member	NNS
1953	1955	of	of	IN
1956	1959	the	the	DT
1960	1972	GATA-binding	gata-binding	JJ
1973	1981	proteins	protein	NNS
1982	1985	are	be	VBP
1986	1995	expressed	express	VBN
1995	1996	.	.	.