0	9	Inducible	inducible	JJ
10	20	expression	expression	NN
21	24	and	and	CC
25	40	phosphorylation	phosphorylation	NN
41	43	of	of	IN
44	55	coactivator	coactivator	NN
56	67	BOB.1/OBF.1	bob.1/obf.1	NN
68	70	in	in	IN
71	72	T	t	NN
73	78	cells	cell	NNS
79	80	[	[	(
80	83	see	see	VB
84	92	comments	comment	NNS
92	93	]	]	)
95	106	BOB.1/OBF.1	BOB.1/OBF.1	NNP
107	109	is	be	VBZ
110	111	a	a	DT
112	127	transcriptional	transcriptional	JJ
128	139	coactivator	coactivator	NN
140	144	that	that	WDT
145	147	is	be	VBZ
148	162	constitutively	constitutively	RB
163	172	expressed	express	VBN
173	175	in	in	IN
176	177	B	B	NNP
178	183	cells	cell	NNS
184	187	and	and	CC
188	197	interacts	interact	VBZ
198	202	with	with	IN
203	206	the	the	DT
207	211	Oct1	oct1	NN
212	215	and	and	CC
216	220	Oct2	oct2	NN
221	234	transcription	transcription	NN
235	242	factors	factor	NNS
242	243	.	.	.

244	248	Upon	upon	IN
249	259	activation	activation	NN
260	262	of	of	IN
263	269	Jurkat	Jurkat	NNP
270	271	T	t	NN
272	277	cells	cell	NNS
278	281	and	and	CC
282	289	primary	primary	JJ
290	296	murine	murine	JJ
297	307	thymocytes	thymocyte	NNS
308	312	with	with	IN
313	320	phorbol	phorbol	NN
321	327	esters	ester	NNS
328	331	and	and	CC
332	341	ionomycin	ionomycin	NN
341	342	,	,	,
343	354	BOB.1/OBF.1	bob.1/obf.1	NN
355	365	expression	expression	NN
366	369	and	and	CC
370	385	transactivation	transactivation	NN
386	394	function	function	NN
395	399	were	be	VBD
400	407	induced	induce	VBN
407	408	.	.	.

409	420	BOB.1/OBF.1	BOB.1/OBF.1	NNP
421	424	was	be	VBD
425	439	phosphorylated	phosphorylate	VBN
440	442	at	at	IN
443	449	Ser184	ser184	NN
450	454	both	both	CC
455	457	in	in	FW
458	462	vivo	vivo	FW
463	466	and	and	CC
467	469	in	in	FW
470	475	vitro	vitro	FW
475	476	,	,	,
477	480	and	and	CC
481	485	this	this	DT
486	498	modification	modification	NN
499	502	was	be	VBD
503	511	required	require	VBN
512	515	for	for	IN
516	525	inducible	inducible	JJ
526	536	activation	activation	NN
536	537	.	.	.

538	546	Mutation	mutation	NN
547	549	of	of	IN
550	556	Ser184	ser184	NN
557	561	also	also	RB
562	572	diminished	diminish	VBD
573	588	transactivation	transactivation	NN
589	597	function	function	NN
598	600	in	in	IN
601	602	B	B	NNP
603	608	cells	cell	NNS
608	609	,	,	,
610	620	suggesting	suggest	VBG
621	625	that	that	IN
626	629	the	the	DT
630	640	activating	activate	VBG
641	656	phosphorylation	phosphorylation	NN
657	661	that	that	WDT
662	664	is	be	VBZ
665	674	inducible	inducible	JJ
675	677	in	in	IN
678	679	T	t	NN
680	685	cells	cell	NNS
686	688	is	be	VBZ
689	703	constitutively	constitutively	RB
704	711	present	present	JJ
712	714	in	in	IN
715	716	B	B	NNP
717	722	cells	cell	NNS
722	723	.	.	.

724	728	Thus	thus	RB
728	729	,	,	,
730	741	BOB.1/OBF.1	BOB.1/OBF.1	NNP
742	744	is	be	VBZ
745	746	a	a	DT
747	762	transcriptional	transcriptional	JJ
763	774	coactivator	coactivator	NN
775	779	that	that	WDT
780	782	is	be	VBZ
783	793	critically	critically	RB
794	803	regulated	regulate	VBN
804	806	by	by	IN
807	824	posttranslational	posttranslational	JJ
825	838	modifications	modification	NNS
839	841	to	to	TO
842	849	mediate	mediate	VB
850	854	cell	cell	NN
855	868	type-specific	type-specific	JJ
869	873	gene	gene	NN
874	884	expression	expression	NN
884	885	.	.	.