0	4	Role	role	NN
5	7	of	of	IN
8	17	ascorbate	ascorbate	NN
18	20	in	in	IN
21	24	the	the	DT
25	35	activation	activation	NN
36	38	of	of	IN
39	48	NF-kappaB	NF-kappaB	NNP
49	51	by	by	IN
52	58	tumour	tumour	NN
59	67	necrosis	necrosis	NN
68	80	factor-alpha	factor-alpha	NN
81	83	in	in	IN
84	91	T-cells	T-cell	NNP
91	92	.	.	.

94	97	The	the	DT
98	103	first	first	JJ
104	111	product	product	NN
112	114	of	of	IN
115	124	ascorbate	ascorbate	NN
125	134	oxidation	oxidation	NN
134	135	,	,	,
136	139	the	the	DT
140	149	ascorbate	ascorbate	NN
150	154	free	free	JJ
155	162	radical	radical	NN
163	164	(	(	(
164	167	AFR	AFR	NNP
167	168	)	)	)
168	169	,	,	,
170	174	acts	act	VBZ
175	177	in	in	IN
178	188	biological	biological	JJ
189	196	systems	system	NNS
197	203	mainly	mainly	RB
204	206	as	as	IN
207	209	an	an	DT
210	217	oxidant	oxidant	NN
217	218	,	,	,
219	222	and	and	CC
223	230	through	through	IN
231	234	its	its	PRP$
235	239	role	role	NN
240	242	in	in	IN
243	246	the	the	DT
247	253	plasma	plasma	NN
254	262	membrane	membrane	NN
263	268	redox	redox	NN
269	275	system	system	NN
276	282	exerts	exert	VBZ
283	292	different	different	JJ
293	300	effects	effect	NNS
301	303	on	on	IN
304	307	the	the	DT
308	312	cell	cell	NN
312	313	.	.	.

314	316	We	we	PRP
317	321	have	have	VBP
322	334	investigated	investigate	VBN
335	338	the	the	DT
339	343	role	role	NN
344	346	of	of	IN
347	356	ascorbate	ascorbate	NN
356	357	,	,	,
358	361	AFR	AFR	NNP
362	365	and	and	CC
366	382	dehydroascorbate	dehydroascorbate	NN
383	384	(	(	(
384	387	DHA	DHA	NNP
387	388	)	)	)
389	391	in	in	IN
392	395	the	the	DT
396	406	activation	activation	NN
407	409	of	of	IN
410	413	the	the	DT
414	423	NF-kappaB	NF-kappaB	NNP
424	437	transcription	transcription	NN
438	444	factor	factor	NN
445	447	in	in	IN
448	454	Jurkat	Jurkat	NNP
455	462	T-cells	T-cell	NNP
463	473	stimulated	stimulate	VBN
474	476	by	by	IN
477	483	tumour	tumour	NN
484	492	necrosis	necrosis	NN
493	505	factor-alpha	factor-alpha	NN
506	507	(	(	(
507	516	TNF-alpha	TNF-alpha	NNP
516	517	)	)	)
517	518	.	.	.

519	523	Here	here	RB
524	526	we	we	PRP
527	531	show	show	VBP
531	532	,	,	,
533	535	by	by	IN
536	551	electrophoretic	electrophoretic	JJ
552	560	mobility	mobility	NN
561	566	shift	shift	NN
567	573	assays	assay	NNS
573	574	,	,	,
575	579	that	that	IN
580	589	ascorbate	ascorbate	NN
590	599	increases	increase	VBZ
600	603	the	the	DT
604	611	binding	binding	NN
612	614	of	of	IN
615	624	NF-kappaB	NF-kappaB	NNP
625	627	to	to	TO
628	631	DNA	DNA	NNP
632	634	in	in	IN
635	655	TNF-alpha-stimulated	tnf-alpha-stimulated	JJ
656	662	Jurkat	Jurkat	NNP
663	668	cells	cell	NNS
668	669	.	.	.

670	673	The	the	DT
674	681	ability	ability	NN
682	684	of	of	IN
685	694	ascorbate	ascorbate	NN
695	697	to	to	TO
698	705	enhance	enhance	VB
706	717	cytoplasmic	cytoplasmic	JJ
718	728	inhibitory	inhibitory	JJ
729	737	IkBalpha	IkBalpha	NNP
738	745	protein	protein	NN
746	757	degradation	degradation	NN
758	768	correlates	correlate	VBZ
769	779	completely	completely	RB
780	784	with	with	IN
785	788	its	its	PRP$
789	797	capacity	capacity	NN
798	800	to	to	TO
801	807	induce	induce	VB
808	817	NF-kappaB	NF-kappaB	NNP
818	825	binding	binding	NN
826	828	to	to	TO
829	832	DNA	DNA	NNP
833	836	and	and	CC
837	839	to	to	TO
840	850	potentiate	potentiate	VB
851	869	NF-kappaB-mediated	nf-kappab-mediated	JJ
870	885	transactivation	transactivation	NN
886	888	of	of	IN
889	892	the	the	DT
893	898	HIV-1	HIV-1	NNP
899	903	long	long	JJ
904	912	terminal	terminal	JJ
913	919	repeat	repeat	NN
920	928	promoter	promoter	NN
929	931	in	in	IN
932	952	TNF-alpha-stimulated	tnf-alpha-stimulated	JJ
953	959	Jurkat	Jurkat	NNP
960	965	cells	cell	NNS
966	969	but	but	CC
970	973	not	not	RB
974	976	in	in	IN
977	982	cells	cell	NNS
983	993	stimulated	stimulate	VBN
994	998	with	with	IN
999	1002	PMA	PMA	NNP
1003	1007	plus	plus	CC
1008	1017	ionomycin	ionomycin	NN
1017	1018	.	.	.

1019	1022	AFR	AFR	NNP
1023	1030	behaves	behave	VBZ
1031	1035	like	like	IN
1036	1045	ascorbate	ascorbate	NN
1045	1046	,	,	,
1047	1052	while	while	IN
1053	1056	DHA	DHA	NNP
1057	1060	and	and	CC
1061	1070	ascorbate	ascorbate	NN
1071	1080	phosphate	phosphate	NN
1081	1083	do	do	VBP
1084	1087	not	not	RB
1088	1094	affect	affect	VB
1095	1113	TNF-alpha-mediated	tnf-alpha-mediated	JJ
1114	1123	NF-kappaB	NF-kappaB	NNP
1124	1134	activation	activation	NN
1134	1135	.	.	.

1136	1141	These	these	DT
1142	1149	results	result	NNS
1150	1157	provide	provide	VBP
1158	1161	new	new	JJ
1162	1170	evidence	evidence	NN
1171	1174	for	for	IN
1175	1176	a	a	DT
1177	1185	possible	possible	JJ
1186	1198	relationship	relationship	NN
1199	1206	between	between	IN
1207	1210	the	the	DT
1211	1221	activation	activation	NN
1222	1224	of	of	IN
1225	1228	the	the	DT
1229	1247	electron-transport	electron-transport	JJ
1248	1254	system	system	NN
1255	1257	at	at	IN
1258	1261	the	the	DT
1262	1268	plasma	plasma	NN
1269	1277	membrane	membrane	NN
1278	1280	by	by	IN
1281	1290	ascorbate	ascorbate	NN
1291	1293	or	or	CC
1294	1297	its	its	PRP$
1298	1302	free	free	JJ
1303	1310	radical	radical	NN
1311	1314	and	and	CC
1315	1330	redox-dependent	redox-dependent	JJ
1331	1335	gene	gene	NN
1336	1349	transcription	transcription	NN
1350	1352	in	in	IN
1353	1360	T-cells	T-cell	NNP
1360	1361	.	.	.