0 3 RP1 RP1 NNP 3 4 , , , 5 6 a a DT 7 10 new new JJ 11 17 member member NN 18 20 of of IN 21 24 the the DT 25 36 adenomatous adenomatous JJ 37 46 polyposis polyposis NN 47 59 coli-binding coli-binding JJ 60 68 EB1-like eb1-like JJ 69 73 gene gene NN 74 80 family family NN 80 81 , , , 82 84 is be VBZ 85 99 differentially differentially RB 100 109 expressed express VBN 110 112 in in IN 113 122 activated activate VBN 123 124 T t NN 125 130 cells cell NNS 130 131 . . . 133 146 Cross-linking cross-linking NN 147 149 of of IN 150 153 the the DT 154 157 CD3 cd3 NN 158 161 and and CC 162 166 CD28 cd28 NN 167 176 molecules molecule NNS 177 179 on on IN 180 181 T t NN 182 193 lymphocytes lymphocyte NNS 194 204 represents represent VBZ 205 208 one one CD 209 211 of of IN 212 215 the the DT 216 220 most most RBS 221 230 effective effective JJ 231 238 signals signal NNS 239 242 for for IN 243 244 T t NN 245 255 lymphocyte lymphocyte NN 256 266 activation activation NN 267 270 and and CC 271 281 triggering triggering NN 282 284 of of IN 285 290 their their PRP$ 291 300 cytotoxic cytotoxic JJ 301 309 effector effector NN 310 318 function function NN 318 319 . . . 320 322 To to TO 323 331 identify identify VB 332 337 genes gene NNS 338 342 that that WDT 343 346 are be VBP 347 356 expressed express VBN 357 359 in in IN 360 361 T t NN 362 367 cells cell NNS 368 373 after after IN 374 385 stimulation stimulation NN 385 386 , , , 387 391 mRNA mrna NN 392 396 from from IN 397 398 T t NN 399 410 lymphocytes lymphocyte NNS 411 415 that that WDT 416 419 had have VBD 420 424 been be VBN 425 434 activated activate VBN 435 437 by by IN 438 441 the the DT 442 454 simultaneous simultaneous JJ 455 466 stimulation stimulation NN 467 469 of of IN 470 473 the the DT 474 477 CD3 cd3 NN 478 481 and and CC 482 486 CD28 cd28 NN 487 494 trigger trigger NN 495 504 molecules molecule NNS 505 508 was be VBD 509 520 transcribed transcribe VBN 521 524 for for IN 525 526 a a DT 527 539 differential differential JJ 540 544 mRNA mrna NN 545 552 display display NN 553 561 analysis analysis NN 562 566 into into IN 567 571 cDNA cDNA NNP 572 575 and and CC 576 579 was be VBD 580 588 compared compare VBN 589 593 with with IN 594 598 cDNA cDNA NNP 599 603 from from IN 604 609 CD28- cd28- JJ 610 612 or or CC 613 626 CD3-activated cd3-activated JJ 627 629 or or CC 630 637 resting rest VBG 638 649 lymphocytes lymphocyte NNS 649 650 . . . 651 663 Differential differential JJ 664 674 expression expression NN 675 678 was be VBD 679 688 confirmed confirm VBN 689 701 subsequently subsequently RB 702 704 by by IN 705 713 Northern northern NN 714 718 blot blot NN 719 727 analysis analysis NN 727 728 . . . 729 732 One one CD 733 735 of of IN 736 739 the the DT 740 744 cDNA cdna NN 745 754 fragments fragment NNS 755 764 expressed express VBN 765 777 specifically specifically RB 778 780 in in IN 781 785 CD3- cd3- NN 786 789 and and CC 790 804 CD28-activated cd28-activated JJ 805 806 T t NN 807 812 cells cell NNS 813 816 was be VBD 817 827 designated designate VBN 828 831 RP1 rp1 NN 831 832 . . . 833 836 The the DT 837 847 predictive predictive JJ 848 862 protein-coding protein-coding JJ 863 869 region region NN 870 872 of of IN 873 876 RP1 RP1 NNP 877 880 had have VBD 881 882 a a DT 883 894 significant significant JJ 895 903 homology homology NN 904 906 to to TO 907 914 members member NNS 915 917 of of IN 918 921 the the DT 922 930 recently recently RB 931 936 found find VBN 937 948 adenomatous adenomatous JJ 949 958 polyposis polyposis NN 959 963 coli coli NN 964 965 ( ( ( 965 968 APC APC NNP 968 969 ) ) ) 970 985 protein-binding protein-binding JJ 986 989 EB1 eb1 NN 990 994 gene gene NN 995 1001 family family NN 1001 1002 , , , 1003 1008 which which WDT 1009 1014 codes cod VBZ 1015 1018 for for IN 1019 1022 yet yet RB 1023 1030 unknown unknown JJ 1031 1041 protein(s) protein(s) NN 1041 1042 . . . 1043 1054 Bacterially bacterially RB 1055 1064 expressed express VBN 1065 1068 RP1 rp1 NN 1069 1076 protein protein NN 1077 1085 revealed reveal VBD 1086 1094 specific specific JJ 1095 1102 binding binding NN 1103 1105 to to TO 1106 1115 wild-type wild-type JJ 1116 1119 but but CC 1120 1123 not not RB 1124 1126 to to TO 1127 1134 mutated mutate VBN 1135 1138 APC APC NNP 1139 1146 protein protein NN 1146 1147 . . . 1148 1151 The the DT 1152 1157 rapid rapid JJ 1158 1171 up-regulation up-regulation NN 1172 1174 of of IN 1175 1178 RP1 RP1 NNP 1179 1183 mRNA mRNA NNP 1184 1186 in in IN 1187 1195 properly properly RB 1196 1205 activated activate VBN 1206 1207 T t NN 1208 1213 cells cell NNS 1214 1222 suggests suggest VBZ 1223 1227 that that IN 1228 1232 this this DT 1233 1237 gene gene NN 1238 1243 might might MD 1244 1250 belong belong VB 1251 1253 to to TO 1254 1257 the the DT 1258 1273 immediate/early immediate/early JJ 1274 1278 gene gene NN 1279 1285 family family NN 1285 1286 , , , 1287 1292 which which WDT 1293 1301 controls control VBZ 1302 1305 the the DT 1306 1312 signal signal NN 1313 1325 transduction transduction NN 1326 1333 cascade cascade NN 1334 1344 downstream downstream JJ 1345 1347 of of IN 1348 1351 the the DT 1352 1355 TCR TCR NNP 1355 1356 . . . 1357 1359 As as IN 1360 1363 the the DT 1364 1374 expression expression NN 1375 1380 level level NN 1381 1383 of of IN 1384 1387 the the DT 1388 1391 RP1 rp1 NN 1392 1396 gene gene NN 1397 1399 in in IN 1400 1409 activated activate VBN 1410 1411 T t NN 1412 1417 cells cell NNS 1418 1421 and and CC 1422 1423 a a DT 1424 1432 spectrum spectrum NN 1433 1435 of of IN 1436 1449 tumor-derived tumor-derived JJ 1450 1454 cell cell NN 1455 1460 lines line NNS 1461 1471 correlates correlate VBZ 1472 1476 with with IN 1477 1480 the the DT 1481 1494 proliferative proliferative JJ 1495 1501 status status NN 1502 1504 of of IN 1505 1508 the the DT 1509 1514 cells cell NNS 1514 1515 , , , 1516 1523 members member NNS 1524 1526 of of IN 1527 1530 the the DT 1531 1539 EB1-like eb1-like JJ 1540 1544 gene gene NN 1545 1551 family family NN 1552 1555 may may MD 1556 1559 not not RB 1560 1564 only only RB 1565 1567 be be VB 1568 1576 involved involve VBN 1577 1579 in in IN 1580 1583 the the DT 1584 1597 tumorigenesis tumorigenesis NN 1598 1600 of of IN 1601 1611 colorectal colorectal JJ 1612 1619 cancers cancer NNS 1620 1623 but but CC 1624 1627 may may MD 1628 1632 also also RB 1633 1637 play play VB 1638 1639 a a DT 1640 1644 role role NN 1645 1647 in in IN 1648 1651 the the DT 1652 1665 proliferative proliferative JJ 1666 1673 control control NN 1674 1676 of of IN 1677 1683 normal normal JJ 1684 1689 cells cell NNS 1689 1690 . . .