0 9 Induction Induction NNP 10 12 of of IN 13 20 nuclear nuclear JJ 21 27 factor factor NN 28 33 kappa kappa NN 34 39 B/Rel B/Rel NNP 40 47 nuclear nuclear JJ 48 56 activity activity NN 57 59 in in IN 60 65 human human JJ 66 76 peripheral peripheral JJ 77 82 blood blood NN 83 84 T t NN 85 96 lymphocytes lymphocyte NNS 97 99 by by IN 100 108 anti-HLA anti-hla JJ 109 114 class class NN 115 116 I i CD 117 127 monoclonal monoclonal JJ 128 138 antibodies antibody NNS 138 139 . . . 141 151 Monoclonal monoclonal JJ 152 162 antibodies antibody NNS 163 170 against against IN 171 177 either either CC 178 189 monomorphic monomorphic JJ 190 192 or or CC 193 204 polymorphic polymorphic JJ 205 217 determinants determinant NNS 218 220 of of IN 221 226 class class NN 227 228 I i CD 229 236 antigen antigen NN 237 244 induced induce VBN 245 247 in in IN 248 252 PBMC PBMC NNP 253 256 and and CC 257 263 highly highly RB 264 272 purified purify VBN 273 274 T t NN 275 286 lymphocytes lymphocyte NNS 287 290 the the DT 291 298 nuclear nuclear JJ 299 307 activity activity NN 308 310 of of IN 311 319 NF-kappa NF-kappa NNP 320 325 B/Rel B/Rel NNP 326 335 complexes complex NNS 335 336 . . . 337 342 These these DT 343 351 included include VBD 352 356 both both CC 357 364 p50/p50 p50/p50 NN 365 368 and and CC 369 376 p50/p65 p50/p65 NN 377 383 dimers dimer NNS 383 384 , , , 385 395 recognized recognize VBN 396 398 by by IN 399 407 specific specific JJ 408 418 antibodies antibody NNS 419 421 in in IN 422 426 EMSA EMSA NNP 426 427 . . . 428 431 The the DT 432 439 induced induce VBN 440 449 complexes complex NNS 450 454 were be VBD 455 465 detectable detectable JJ 466 468 in in IN 469 477 extracts extract NNS 478 480 of of IN 481 486 cells cell NNS 487 496 incubated incubate VBN 497 501 with with IN 502 512 anti-class anti-class JJ 513 514 I i CD 515 525 monoclonal monoclonal JJ 526 534 antibody antibody NN 535 536 ( ( ( 536 539 mAb mab NN 539 540 ) ) ) 541 544 for for IN 545 548 1.5 1.5 CD 549 550 h h NN 550 551 ; ; : 552 555 the the DT 556 565 induction induction NN 566 569 was be VBD 570 577 maximal maximal JJ 578 580 at at IN 581 582 5 5 CD 583 584 h h NN 584 585 , , , 586 596 persistent persistent JJ 597 599 at at IN 600 602 16 16 CD 603 604 h h NN 605 608 and and CC 609 611 no no RB 612 618 longer longer RBR 619 627 observed observe VBN 628 630 at at IN 631 633 40 40 CD 634 635 h h NN 635 636 . . . 637 640 The the DT 641 644 mAb mab NN 645 651 failed fail VBD 652 654 to to TO 655 661 induce induce VB 662 670 NF-kappa NF-kappa NNP 671 676 B/Rel B/Rel NNP 677 684 nuclear nuclear JJ 685 693 activity activity NN 694 696 in in IN 697 702 cells cell NNS 703 712 incubated incubate VBN 713 715 in in IN 716 719 the the DT 720 728 presence presence NN 729 731 of of IN 732 755 3,4-dichloroisocoumarin 3,4-dichloroisocoumarin NN 755 756 , , , 757 759 an an DT 760 769 inhibitor inhibitor NN 770 772 of of IN 773 774 I i NN 775 780 kappa kappa NN 781 788 B-alpha B-alpha NNP 789 800 degradation degradation NN 800 801 . . . 802 810 Together together RB 810 811 , , , 812 817 these these DT 818 825 results result NNS 826 833 suggest suggest VBP 834 838 that that IN 839 844 class class NN 845 846 I i CD 847 857 triggering triggering NN 858 861 can can MD 862 868 induce induce VB 869 872 the the DT 873 881 activity activity NN 882 884 of of IN 885 893 NF-kappa NF-kappa NNP 894 899 B/Rel B/Rel NNP 900 907 nuclear nuclear JJ 908 916 activity activity NN 917 919 in in IN 920 930 peripheral peripheral JJ 931 936 blood blood NN 937 938 T t NN 939 950 lymphocytes lymphocyte NNS 950 951 , , , 952 959 thereby thereby RB 960 970 modulating modulate VBG 971 974 the the DT 975 985 expression expression NN 986 988 of of IN 989 994 genes gene NNS 995 1004 regulated regulate VBN 1005 1007 by by IN 1008 1013 these these DT 1014 1027 transcription transcription NN 1028 1035 factors factor NNS 1035 1036 . . .