0 3 EBF EBF NNP 4 7 and and CC 8 11 E47 E47 NNP 12 23 collaborate collaborate VBP 24 26 to to TO 27 33 induce induce VB 34 44 expression expression NN 45 47 of of IN 48 51 the the DT 52 62 endogenous endogenous JJ 63 77 immunoglobulin immunoglobulin NN 78 87 surrogate surrogate JJ 88 93 light light JJ 94 99 chain chain NN 100 105 genes gene NNS 105 106 . . . 108 113 Early early JJ 114 115 B B NNP 116 120 cell cell NN 121 127 factor factor NN 128 129 ( ( ( 129 132 EBF EBF NNP 132 133 ) ) ) 134 137 and and CC 138 141 E47 E47 NNP 142 153 participate participate VBP 154 156 in in IN 157 160 the the DT 161 176 transcriptional transcriptional JJ 177 184 control control NN 185 187 of of IN 188 193 early early JJ 194 195 B B NNP 196 206 lymphocyte lymphocyte NN 207 222 differentiation differentiation NN 222 223 . . . 224 228 With with IN 229 232 the the DT 233 236 aim aim NN 237 239 of of IN 240 251 identifying identify VBG 252 259 genetic genetic JJ 260 267 targets target NNS 268 271 for for IN 272 277 these these DT 278 291 transcription transcription NN 292 299 factors factor NNS 299 300 , , , 301 303 we we PRP 304 310 stably stably RB 311 322 transfected transfecte VBD 323 328 cDNAs cdna NNS 329 337 encoding encode VBG 338 341 EBF EBF NNP 342 344 or or CC 345 346 a a DT 347 355 covalent covalent JJ 356 365 homodimer homodimer NN 366 368 of of IN 369 372 E47 E47 NNP 372 373 , , , 374 386 individually individually RB 387 389 or or CC 390 398 together together RB 398 399 , , , 400 404 into into IN 405 413 immature immature JJ 414 427 hematopoietic hematopoietic JJ 428 433 Ba/F3 ba/f3 NN 434 439 cells cell NNS 439 440 , , , 441 446 which which WDT 447 451 lack lack VBP 452 456 both both DT 457 464 factors factor NNS 464 465 . . . 466 468 In in IN 469 480 combination combination NN 480 481 , , , 482 485 EBF EBF NNP 486 489 and and CC 490 493 E47 E47 NNP 494 500 induce induce VBP 501 510 efficient efficient JJ 511 521 expression expression NN 522 524 of of IN 525 528 the the DT 529 539 endogenous endogenous JJ 540 554 immunoglobulin immunoglobulin NN 555 564 surrogate surrogate JJ 565 570 light light JJ 571 576 chain chain NN 577 582 genes gene NNS 582 583 , , , 584 591 lambda5 lambda5 NN 592 595 and and CC 596 601 VpreB VpreB NNP 601 602 , , , 603 610 whereas whereas IN 611 616 other other JJ 617 622 pre-B pre-b JJ 623 636 cell-specific cell-specific JJ 637 642 genes gene NNS 643 649 remain remain VBP 650 656 silent silent JJ 656 657 . . . 658 666 Multiple multiple JJ 667 679 functionally functionally RB 680 689 important important JJ 690 693 EBF EBF NNP 694 697 and and CC 698 701 E47 E47 NNP 702 709 binding binding NN 710 715 sites site NNS 716 720 were be VBD 721 731 identified identify VBN 732 734 in in IN 735 738 the the DT 739 746 lambda5 lambda5 NN 747 764 promoter/enhancer promoter/enhancer NN 765 771 region region NN 771 772 , , , 773 783 indicating indicate VBG 784 788 that that IN 789 796 lambda5 lambda5 NN 797 799 is be VBZ 800 801 a a DT 802 808 direct direct JJ 809 816 genetic genetic JJ 817 823 target target NN 824 827 for for IN 828 833 these these DT 834 847 transcription transcription NN 848 855 factors factor NNS 855 856 . . . 857 862 Taken take VBN 863 871 together together RB 871 872 , , , 873 878 these these DT 879 883 data datum NNS 884 891 suggest suggest VBP 892 896 that that IN 897 900 EBF EBF NNP 901 904 and and CC 905 908 E47 E47 NNP 909 918 synergize synergize VBP 919 921 to to TO 922 930 activate activate VB 931 941 expression expression NN 942 944 of of IN 945 946 a a DT 947 953 subset subset NN 954 956 of of IN 957 962 genes gene NNS 963 967 that that WDT 968 974 define define VBP 975 977 an an DT 978 983 early early JJ 984 989 stage stage NN 990 992 of of IN 993 996 the the DT 997 998 B B NNP 999 1003 cell cell NN 1004 1011 lineage lineage NN 1011 1012 . . .