0 7 Nuclear nuclear JJ 8 14 levels level NNS 15 17 of of IN 18 27 NF-kappaB NF-kappaB NNP 28 37 correlate correlate VBP 38 42 with with IN 43 60 syncytium-forming syncytium-forming JJ 61 69 capacity capacity NN 70 72 of of IN 73 77 8e51 8e51 NN 78 83 cells cell NNS 83 84 , , , 85 95 expressing express VBG 96 97 a a DT 98 107 defective defective JJ 108 111 HIV HIV NNP 112 117 virus virus NN 117 118 . . . 120 123 The the DT 124 130 double double JJ 131 140 NF-kappaB nf-kappab NN 141 145 site site NN 146 156 identified identify VBN 157 159 in in IN 160 163 the the DT 164 167 LTR ltr NN 168 170 of of IN 171 174 the the DT 175 180 human human JJ 181 197 immunodeficiency immunodeficiency NN 198 205 virus-1 virus-1 NN 206 207 ( ( ( 207 212 HIV-1 HIV-1 NNP 212 213 ) ) ) 214 217 has have VBZ 218 222 been be VBN 223 235 demonstrated demonstrate VBN 236 238 to to TO 239 241 be be VB 242 251 necessary necessary JJ 252 255 for for IN 256 265 efficient efficient JJ 266 271 viral viral JJ 272 285 transcription transcription NN 285 286 . . . 287 289 In in IN 290 294 this this DT 295 301 report report NN 302 304 we we PRP 305 312 present present VBP 313 316 the the DT 317 333 characterisation characterisation NN 334 336 of of IN 337 346 NF-kappaB NF-kappaB NNP 347 355 subunits subunit NNS 356 363 engaged engage VBN 364 366 in in IN 367 376 complexes complex NNS 377 384 binding bind VBG 385 387 to to TO 388 391 the the DT 392 397 HIV-1 HIV-1 NNP 398 407 NF-kappaB NF-kappaB NNP 408 412 site site NN 413 415 in in IN 416 421 human human JJ 422 426 8e51 8e51 NN 427 434 T-cells t-cell NNS 434 435 , , , 436 440 that that WDT 441 448 harbour harbour VBP 449 450 a a DT 451 460 defective defective JJ 461 466 HIV-1 HIV-1 NNP 466 467 . . . 468 470 At at IN 471 476 least least JJS 477 481 four four CD 482 491 different different JJ 492 500 specific specific JJ 501 510 NF-kappaB nf-kappab NN 511 520 complexes complex NNS 521 524 are be VBP 525 532 present present JJ 533 535 in in IN 536 539 the the DT 540 547 nucleus nucleus NN 548 550 of of IN 551 556 these these DT 557 562 cells cell NNS 562 563 . . . 564 568 With with IN 569 572 the the DT 573 576 use use NN 577 579 of of IN 580 588 specific specific JJ 589 599 antibodies antibody NNS 600 602 we we PRP 603 607 have have VBP 608 618 determined determine VBN 619 622 the the DT 623 634 composition composition NN 635 637 of of IN 638 642 each each DT 643 650 complex complex NN 651 656 using use VBG 657 672 electrophoretic electrophoretic JJ 673 681 mobility mobility NN 682 687 shift shift NN 688 694 assays assay NNS 694 695 . . . 696 699 The the DT 700 707 results result NNS 708 712 show show VBP 713 716 the the DT 717 725 presence presence NN 726 728 of of IN 729 736 several several JJ 737 746 NF-kappaB NF-kappaB NNP 747 753 family family NN 754 761 members member NNS 761 762 , , , 763 767 with with IN 768 771 the the DT 772 787 transactivating transactivate VBG 788 792 RelA rela NN 793 798 being be VBG 799 806 engaged engage VBN 807 809 in in IN 810 818 multiple multiple JJ 819 828 complexes complex NNS 828 829 . . . 830 833 The the DT 834 844 importance importance NN 845 847 of of IN 848 857 NF-kappaB NF-kappaB NNP 858 867 complexes complex NNS 868 870 in in IN 871 876 viral viral JJ 877 886 functions function NNS 887 890 has have VBZ 891 895 been be VBN 896 907 established establish VBN 908 917 comparing compare VBG 918 921 the the DT 922 927 level level NN 928 930 of of IN 931 940 NF-kappaB NF-kappaB NNP 941 952 DNA-binding dna-binding JJ 953 962 complexes complex NNS 963 967 with with IN 968 984 syncytia-forming syncytia-forming JJ 985 993 activity activity NN 994 996 of of IN 997 1001 8e51 8e51 NN 1002 1007 cells cell NNS 1007 1008 . . . 1009 1011 In in IN 1012 1016 fact fact NN 1016 1017 , , , 1018 1022 8e51 8e51 NN 1023 1028 cells cell NNS 1029 1033 that that WDT 1034 1037 had have VBD 1038 1044 almost almost RB 1045 1049 lost lose VBN 1050 1055 their their PRP$ 1056 1072 syncytia-forming syncytia-forming JJ 1073 1081 capacity capacity NN 1082 1086 were be VBD 1087 1092 found find VBN 1093 1095 to to TO 1096 1103 contain contain VB 1104 1106 at at IN 1107 1112 least least JJS 1113 1115 10 10 CD 1116 1121 times time NNS 1122 1126 less less RBR 1127 1133 active active JJ 1134 1143 NF-kappaB nf-kappab NN 1144 1155 DNA-binding dna-binding JJ 1156 1163 complex complex NN 1164 1168 than than IN 1169 1172 the the DT 1173 1181 actively actively RB 1182 1188 fusing fuse VBG 1189 1194 cells cell NNS 1194 1195 . . . 1196 1199 The the DT 1200 1211 correlation correlation NN 1212 1214 is be VBZ 1215 1223 specific specific JJ 1224 1226 as as IN 1227 1230 the the DT 1231 1236 level level NN 1237 1239 of of IN 1240 1242 at at IN 1243 1248 least least JJS 1249 1254 three three CD 1255 1260 other other JJ 1261 1274 transcription transcription NN 1275 1282 factors factor NNS 1283 1286 did do VBD 1287 1290 not not RB 1291 1297 change change VB 1297 1298 . . .