0	4	Four	four	CD
5	11	P-like	p-like	JJ
12	20	elements	element	NNS
21	24	are	be	VBP
25	33	required	require	VBN
34	37	for	for	IN
38	45	optimal	optimal	JJ
46	59	transcription	transcription	NN
60	62	of	of	IN
63	66	the	the	DT
67	72	mouse	mouse	NN
73	77	IL-4	il-4	NN
78	82	gene	gene	NN
82	83	:	:	:
84	95	involvement	involvement	NN
96	98	of	of	IN
99	100	a	a	DT
101	109	distinct	distinct	JJ
110	113	set	set	NN
114	116	of	of	IN
117	124	nuclear	nuclear	JJ
125	131	factor	factor	NN
132	134	of	of	IN
135	144	activated	activate	VBN
145	146	T	t	NN
147	152	cells	cell	NNS
153	156	and	and	CC
157	166	activator	activator	NN
167	176	protein-1	protein-1	NN
177	183	family	family	NN
184	192	proteins	protein	NNS
192	193	.	.	.

195	197	We	we	PRP
198	208	previously	previously	RB
209	219	identified	identify	VBD
220	223	the	the	DT
224	225	P	p	NN
226	234	sequence	sequence	NN
235	237	as	as	IN
238	239	a	a	DT
240	248	critical	critical	JJ
249	259	regulatory	regulatory	JJ
260	267	element	element	NN
268	270	of	of	IN
271	274	the	the	DT
275	280	human	human	JJ
281	285	IL-4	il-4	NN
286	294	promoter	promoter	NN
294	295	.	.	.

296	298	In	in	IN
299	302	the	the	DT
303	308	mouse	mouse	NN
309	313	IL-4	il-4	NN
314	322	promoter	promoter	NN
322	323	,	,	,
324	329	there	there	EX
330	333	are	be	VBP
334	338	five	five	CD
339	347	elements	element	NNS
348	358	homologous	homologous	JJ
359	361	to	to	TO
362	365	the	the	DT
366	371	human	human	JJ
372	373	P	p	NN
374	382	sequence	sequence	NN
383	393	designated	designate	VBN
394	403	conserved	conserve	VBN
404	414	lymphokine	lymphokine	NN
415	422	element	element	NN
423	424	0	0	CD
425	426	(	(	(
426	430	CLE0	CLE0	NNP
430	431	)	)	)
431	432	,	,	,
433	434	P	p	NN
434	435	,	,	,
436	438	P2	P2	NNP
438	439	,	,	,
440	442	P3	P3	NNP
443	446	and	and	CC
447	449	P4	P4	NNP
449	450	.	.	.

451	453	To	to	TO
454	466	characterize	characterize	VB
467	470	the	the	DT
471	475	role	role	NN
476	478	of	of	IN
479	484	these	these	DT
485	491	P-like	p-like	JJ
492	500	elements	element	NNS
501	504	and	and	CC
505	510	their	their	PRP$
511	518	binding	binding	NN
519	526	factors	factor	NNS
527	529	in	in	IN
530	533	the	the	DT
534	540	native	native	JJ
541	549	promoter	promoter	NN
549	550	,	,	,
551	553	we	we	PRP
554	557	did	do	VBD
558	567	transient	transient	JJ
568	580	transfection	transfection	NN
581	584	and	and	CC
585	600	electrophoretic	electrophoretic	JJ
601	609	mobility	mobility	NN
610	615	shift	shift	NN
616	622	assays	assay	NNS
623	624	(	(	(
624	628	EMSA	EMSA	NNP
628	629	)	)	)
629	630	.	.	.

631	643	Transfection	transfection	NN
644	646	of	of	IN
647	651	EL-4	el-4	NN
652	657	cells	cell	NNS
658	662	with	with	IN
663	666	the	the	DT
667	671	IL-4	il-4	NN
672	689	promoter-reporter	promoter-reporter	JJ
690	700	constructs	construct	NNS
701	709	carrying	carry	VBG
710	717	mutated	mutate	VBN
718	724	P-like	p-like	JJ
725	733	elements	element	NNS
734	740	showed	show	VBD
741	745	that	that	IN
746	750	four	four	CD
751	757	P-like	p-like	JJ
758	766	elements	element	NNS
766	767	,	,	,
768	772	CLE0	CLE0	NNP
772	773	,	,	,
774	775	P	p	NN
775	776	,	,	,
777	779	P2	p2	NN
780	783	and	and	CC
784	786	P4	p4	NN
786	787	,	,	,
788	791	but	but	CC
792	795	not	not	RB
796	798	P3	p3	NN
798	799	,	,	,
800	804	were	be	VBD
805	813	required	require	VBN
814	817	for	for	IN
818	825	optimal	optimal	JJ
826	836	activation	activation	NN
837	839	of	of	IN
840	843	the	the	DT
844	848	IL-4	il-4	NN
849	857	promoter	promoter	NN
857	858	.	.	.

859	863	EMSA	EMSA	NNP
864	870	showed	show	VBD
871	875	that	that	IN
876	880	both	both	CC
881	893	constitutive	constitutive	JJ
894	897	and	and	CC
898	907	inducible	inducible	JJ
908	917	complexes	complex	NNS
918	923	bound	bind	VBN
924	926	to	to	TO
927	931	CLE0	CLE0	NNP
931	932	,	,	,
933	934	P	p	NN
934	935	,	,	,
936	938	P2	p2	NN
939	942	and	and	CC
943	945	P4	p4	NN
945	946	,	,	,
947	954	whereas	whereas	IN
955	959	only	only	RB
960	961	a	a	DT
962	974	constitutive	constitutive	JJ
975	982	complex	complex	NN
983	988	bound	bind	VBD
989	991	to	to	TO
992	994	P3	P3	NNP
994	995	.	.	.

996	998	In	in	IN
999	1010	competition	competition	NN
1011	1014	and	and	CC
1015	1023	antibody	antibody	NN
1024	1034	supershift	supershift	NN
1035	1041	assays	assay	NNS
1042	1044	in	in	IN
1045	1049	EMSA	EMSA	NNP
1049	1050	,	,	,
1051	1060	complexes	complex	NNS
1061	1067	formed	form	VBN
1068	1072	with	with	IN
1073	1074	P	p	NN
1075	1077	or	or	CC
1078	1080	P2	p2	NN
1081	1087	proved	prove	VBD
1088	1090	to	to	TO
1091	1098	contain	contain	VB
1099	1106	nuclear	nuclear	JJ
1107	1113	factor	factor	NN
1114	1116	of	of	IN
1117	1126	activated	activate	VBN
1127	1128	T	t	NN
1129	1134	cells	cell	NNS
1135	1136	(	(	(
1136	1140	NFAT	NFAT	NNP
1140	1141	)	)	)
1142	1148	family	family	NN
1149	1157	proteins	protein	NNS
1158	1160	as	as	IN
1161	1166	major	major	JJ
1167	1177	components	component	NNS
1177	1178	.	.	.

1179	1188	Activator	Activator	NNP
1189	1196	protein	protein	NN
1197	1198	(	(	(
1198	1203	AP)-1	ap)-1	NN
1204	1210	family	family	NN
1211	1219	proteins	protein	NNS
1220	1230	interacted	interact	VBD
1231	1235	with	with	IN
1236	1240	CLE0	CLE0	NNP
1240	1241	,	,	,
1242	1243	P	p	NN
1243	1244	,	,	,
1245	1247	P2	p2	NN
1248	1251	and	and	CC
1252	1254	P4	p4	NN
1254	1255	.	.	.

1256	1265	NFAT/AP-1	nfat/ap-1	NN
1266	1273	complex	complex	NN
1274	1280	formed	form	VBD
1281	1285	only	only	RB
1286	1290	with	with	IN
1291	1292	P	p	NN
1293	1296	and	and	CC
1297	1299	P2	p2	NN
1299	1300	.	.	.

1301	1318	Cross-competition	cross-competition	NN
1319	1325	assays	assay	NNS
1326	1331	among	among	IN
1332	1335	the	the	DT
1336	1342	P-like	p-like	JJ
1343	1351	elements	element	NNS
1352	1360	revealed	reveal	VBD
1361	1377	element-specific	element-specific	JJ
1378	1381	and	and	CC
1382	1388	common	common	JJ
1389	1398	complexes	complex	NNS
1398	1399	.	.	.

1400	1403	Six	six	CD
1404	1410	tandem	tandem	JJ
1411	1418	repeats	repeat	NNS
1419	1421	of	of	IN
1422	1425	the	the	DT
1426	1427	P	p	NN
1428	1435	element	element	NN
1436	1442	linked	link	VBN
1443	1445	to	to	TO
1446	1449	the	the	DT
1450	1454	SV40	SV40	NNP
1455	1463	promoter	promoter	NN
1464	1473	responded	respond	VBD
1474	1476	to	to	TO
1477	1484	phorbol	phorbol	NN
1485	1497	12-myristate	12-myristate	NN
1498	1508	13-acetate	13-acetate	NN
1508	1509	,	,	,
1510	1515	while	while	IN
1516	1520	that	that	DT
1521	1523	of	of	IN
1524	1529	other	other	JJ
1530	1538	elements	element	NNS
1539	1542	did	do	VBD
1543	1546	not	not	RB
1546	1547	.	.	.

1548	1550	It	it	PRP
1551	1556	would	would	MD
1557	1561	thus	thus	RB
1562	1568	appear	appear	VB
1569	1573	that	that	IN
1574	1584	components	component	NNS
1585	1587	of	of	IN
1588	1592	each	each	DT
1593	1599	P-like	p-like	JJ
1600	1615	element-binding	element-binding	JJ
1616	1625	complexes	complex	NNS
1626	1629	are	be	VBP
1630	1633	not	not	RB
1634	1643	identical	identical	JJ
1644	1647	and	and	CC
1648	1651	may	may	MD
1652	1664	coordinately	coordinately	RB
1665	1675	contribute	contribute	VB
1676	1678	to	to	TO
1679	1694	transcriptional	transcriptional	JJ
1695	1703	activity	activity	NN
1703	1704	.	.	.