0 5 Human human JJ 6 17 neutrophils neutrophil NNS 18 25 express express VBP 26 30 GH-N gh-n NN 31 35 gene gene NN 36 47 transcripts transcript NNS 48 51 and and CC 52 55 the the DT 56 65 pituitary pituitary JJ 66 79 transcription transcription NN 80 86 factor factor NN 87 93 Pit-1b Pit-1b NNP 93 94 . . . 96 101 Since since IN 102 104 GH GH NNP 105 115 stimulates stimulate VBZ 116 119 the the DT 120 131 development development NN 132 135 and and CC 136 144 function function NN 145 147 of of IN 148 160 granulocytes granulocyte NNS 160 161 , , , 162 164 we we PRP 165 177 investigated investigate VBD 178 181 the the DT 182 192 expression expression NN 193 195 of of IN 196 198 GH GH NNP 199 201 in in IN 202 213 granulocyte granulocyte NN 214 221 subsets subset NNS 221 222 . . . 223 225 By by IN 226 245 immunocytochemistry immunocytochemistry NN 245 246 , , , 247 249 25 25 CD 250 253 +/- +/- SYM 254 255 7 7 CD 255 256 % % NN 257 259 of of IN 260 263 the the DT 264 269 human human JJ 270 281 neutrophils neutrophil NNS 282 286 were be VBD 287 292 shown show VBN 293 295 to to TO 296 303 express express VB 304 318 immunoreactive immunoreactive JJ 319 321 GH gh NN 321 322 , , , 323 330 whereas whereas IN 331 342 eosinophils eosinophil NNS 343 347 were be VBD 348 356 negative negative JJ 356 357 . . . 358 366 Reversed reverse VBN 367 380 transcription transcription NN 381 382 ( ( ( 382 389 RT)-PCR RT)-PCR NNP 390 398 analysis analysis NN 399 411 demonstrated demonstrate VBD 412 414 GH GH NNP 415 419 mRNA mRNA NNP 420 422 in in IN 423 434 neutrophils neutrophil NNS 434 435 . . . 436 447 Restriction restriction NN 448 456 analysis analysis NN 457 465 revealed reveal VBD 466 470 that that IN 471 482 neutrophils neutrophil NNS 483 490 express express VBP 491 494 the the DT 495 499 GH-N GH-N NNP 500 504 gene gene NN 505 508 but but CC 509 512 not not RB 513 516 the the DT 517 521 GH-V GH-V NNP 522 526 gene gene NN 526 527 . . . 528 539 Furthermore furthermore RB 539 540 , , , 541 543 we we PRP 544 556 demonstrated demonstrate VBD 557 559 by by IN 560 567 western western JJ 568 572 blot blot NN 573 581 analysis analysis NN 582 586 that that IN 587 598 neutrophils neutrophil NNS 599 606 express express VBP 607 609 an an DT 610 623 alternatively alternatively RB 624 631 spliced splice VBN 632 639 variant variant NN 640 642 of of IN 643 646 the the DT 647 656 pituitary pituitary JJ 657 670 transcription transcription NN 671 677 factor factor NN 678 683 Pit-1 pit-1 NN 683 684 , , , 685 695 designated designate VBN 696 702 Pit-1b Pit-1b NNP 702 703 . . .