0 3 The the DT 4 11 spatial spatial JJ 12 24 distribution distribution NN 25 27 of of IN 28 33 human human JJ 34 48 immunoglobulin immunoglobulin NN 49 54 genes gene NNS 55 61 within within IN 62 65 the the DT 66 73 nucleus nucleus NN 73 74 : : : 75 83 evidence evidence NN 84 87 for for IN 88 92 gene gene NN 93 103 topography topography NN 104 115 independent independent JJ 116 118 of of IN 119 123 cell cell NN 124 128 type type NN 129 132 and and CC 133 148 transcriptional transcriptional JJ 149 157 activity activity NN 157 158 . . . 160 163 The the DT 164 181 three-dimensional three-dimensional JJ 182 193 positioning positioning NN 194 196 of of IN 197 211 immunoglobulin immunoglobulin NN 212 213 ( ( ( 213 215 Ig Ig NNP 215 216 ) ) ) 217 222 genes gene NNS 223 229 within within IN 230 233 the the DT 234 241 nucleus nucleus NN 242 244 of of IN 245 250 human human JJ 251 256 cells cell NNS 257 260 was be VBD 261 273 investigated investigate VBN 274 279 using use VBG 280 282 in in FW 283 287 situ situ FW 288 301 hybridization hybridization NN 302 305 and and CC 306 314 confocal confocal JJ 315 325 microscopy microscopy NN 325 326 . . . 327 330 The the DT 331 344 visualization visualization NN 345 347 of of IN 348 353 heavy heavy JJ 354 357 and and CC 358 363 light light JJ 364 369 chain chain NN 370 375 genes gene NNS 376 378 in in IN 379 389 B-lymphoid b-lymphoid JJ 390 395 cells cell NNS 396 402 showed show VBD 403 407 that that IN 408 411 the the DT 412 417 three three CD 418 420 Ig Ig NNP 421 426 genes gene NNS 427 430 are be VBP 431 445 differentially differentially RB 446 449 and and CC 450 461 nonrandomly nonrandomly RB 462 473 distributed distribute VBN 474 476 in in IN 477 486 different different JJ 487 494 nuclear nuclear JJ 495 505 subvolumes subvolume NNS 505 506 : : : 507 510 the the DT 511 516 kappa kappa NN 517 522 genes gene NNS 523 527 were be VBD 528 533 found find VBN 534 536 to to TO 537 539 be be VB 540 554 preferentially preferentially RB 555 563 confined confine VBN 564 566 to to TO 567 569 an an DT 570 575 outer outer JJ 576 583 nuclear nuclear JJ 584 590 volume volume NN 590 591 , , , 592 599 whereas whereas IN 600 603 the the DT 604 609 gamma gamma NN 610 613 and and CC 614 620 lambda lambda NN 621 626 genes gene NNS 627 639 consistently consistently RB 640 648 occupied occupy VBD 649 653 more more RBR 654 661 central central JJ 662 671 positions position NNS 672 678 within within IN 679 682 the the DT 683 690 nucleus nucleus NN 690 691 , , , 692 695 the the DT 696 702 lambda lambda NN 703 708 genes gene NNS 709 714 being be VBG 715 719 more more RBR 720 728 interior interior JJ 729 733 when when WRB 734 742 compared compare VBN 743 747 with with IN 748 751 the the DT 752 757 gamma gamma NN 758 763 genes gene NNS 763 764 . . . 765 768 The the DT 769 773 data datum NNS 774 781 further further RB 782 786 show show VBP 787 791 that that IN 792 797 these these DT 798 805 overall overall JJ 806 819 topographical topographical JJ 820 833 distributions distribution NNS 834 837 are be VBP 838 849 independent independent JJ 850 852 of of IN 853 857 gene gene NN 858 873 transcriptional transcriptional JJ 874 882 activity activity NN 883 886 and and CC 887 890 are be VBP 891 900 conserved conserve VBN 901 903 in in IN 904 913 different different JJ 914 918 cell cell NN 919 924 types type NNS 924 925 . . . 926 934 Although although IN 935 941 subtle subtle JJ 942 946 gene gene NN 947 956 movements movement NNS 957 963 within within IN 964 969 those those DT 970 977 defined define VBN 978 991 topographical topographical JJ 992 999 regions region NNS 1000 1006 cannot cannot MD 1007 1009 be be VB 1010 1018 excluded exclude VBN 1019 1021 by by IN 1022 1026 this this DT 1027 1032 study study NN 1032 1033 , , , 1034 1037 the the DT 1038 1045 results result NNS 1046 1054 indicate indicate VBP 1055 1059 that that IN 1060 1066 tissue tissue NN 1067 1078 specificity specificity NN 1079 1081 of of IN 1082 1086 gene gene NN 1087 1097 expression expression NN 1098 1100 is be VBZ 1101 1104 not not RB 1105 1116 accompanied accompany VBN 1117 1119 by by IN 1120 1127 drastic drastic JJ 1128 1135 changes change NNS 1136 1138 in in IN 1139 1143 gene gene NN 1144 1151 nuclear nuclear JJ 1152 1162 topography topography NN 1162 1163 , , , 1164 1170 rather rather RB 1171 1181 suggesting suggest VBG 1182 1186 that that IN 1187 1191 gene gene NN 1192 1204 organization organization NN 1205 1211 within within IN 1212 1215 the the DT 1216 1223 nucleus nucleus NN 1224 1227 may may MD 1228 1230 be be VB 1231 1240 primarily primarily RB 1241 1250 dependent dependent JJ 1251 1253 on on IN 1254 1264 structural structural JJ 1265 1276 constraints constraint NNS 1277 1284 imposed impose VBN 1285 1287 on on IN 1288 1291 the the DT 1292 1302 respective respective JJ 1303 1314 chromosomes chromosome NNS 1314 1315 . . .