0 15 Transcriptional transcriptional JJ 16 26 activation activation NN 27 29 of of IN 30 33 the the DT 34 42 vascular vascular JJ 43 47 cell cell NN 48 56 adhesion adhesion NN 57 67 molecule-1 molecule-1 NN 68 72 gene gene NN 73 75 in in IN 76 77 T t NN 78 89 lymphocytes lymphocyte NNS 90 100 expressing express VBG 101 106 human human JJ 107 113 T-cell t-cell NN 114 122 leukemia leukemia NN 123 128 virus virus NN 129 133 type type NN 134 135 1 1 CD 136 139 Tax tax NN 140 147 protein protein NN 147 148 . . . 150 161 Recruitment recruitment NN 162 165 and and CC 166 179 extravasation extravasation NN 180 182 of of IN 183 184 T t NN 185 190 cells cell NNS 191 198 through through IN 199 202 the the DT 203 214 blood-brain blood-brain NN 215 222 barrier barrier NN 223 226 are be VBP 227 234 favored favor VBN 235 237 by by IN 238 246 adhesion adhesion NN 247 264 molecule-mediated molecule-mediated JJ 265 277 interactions interaction NNS 278 280 of of IN 281 292 circulating circulate VBG 293 294 T t NN 295 300 cells cell NNS 301 305 with with IN 306 317 endothelial endothelial JJ 318 323 cells cell NNS 323 324 . . . 325 330 Since since IN 331 332 a a DT 333 339 common common JJ 340 352 pathological pathological JJ 353 360 finding finding NN 361 363 in in IN 364 369 human human JJ 370 376 T-cell t-cell NN 377 385 leukemia leukemia NN 386 391 virus virus NN 392 396 type type NN 397 398 1 1 CD 399 400 ( ( ( 400 418 HTLV-1)-associated htlv-1)-associated JJ 419 427 diseases disease NNS 428 430 is be VBZ 431 434 the the DT 435 447 infiltration infiltration NN 448 450 of of IN 451 466 HTLV-1-infected htlv-1-infected JJ 467 468 T t NN 469 480 lymphocytes lymphocyte NNS 481 485 into into IN 486 493 various various JJ 494 500 organs organ NNS 500 501 , , , 502 504 we we PRP 505 509 have have VBP 510 516 looked look VBN 517 520 for for IN 521 524 the the DT 525 532 profile profile NN 533 535 of of IN 536 544 adhesion adhesion NN 545 554 molecules molecule NNS 555 564 expressed express VBN 565 567 by by IN 568 586 HTLV-1-transformed htlv-1-transformed JJ 587 588 T t NN 589 594 cells cell NNS 594 595 . . . 596 600 Flow Flow NNP 601 610 cytometry cytometry NN 611 619 analysis analysis NN 620 629 indicated indicate VBD 630 634 that that IN 635 640 these these DT 641 646 cells cell NNS 647 651 were be VBD 652 662 expressing express VBG 663 667 high high JJ 668 674 levels level NNS 675 677 of of IN 678 686 vascular vascular JJ 687 691 cell cell NN 692 700 adhesion adhesion NN 701 709 molecule molecule NN 710 711 1 1 CD 712 713 ( ( ( 713 719 VCAM-1 VCAM-1 NNP 720 721 [ [ ( 721 726 CD106 CD106 NNP 726 727 ] ] ) 727 728 ) ) ) 728 729 , , , 730 731 a a DT 732 739 110-kDa 110-kda JJ 740 746 member member NN 747 749 of of IN 750 753 the the DT 754 768 immunoglobulin immunoglobulin NN 769 773 gene gene NN 774 785 superfamily superfamily NN 785 786 , , , 787 792 first first RB 793 803 identified identify VBN 804 806 on on IN 807 818 endothelial endothelial JJ 819 824 cells cell NNS 825 835 stimulated stimulate VBN 836 840 with with IN 841 853 inflammatory inflammatory JJ 854 863 cytokines cytokine NNS 863 864 . . . 865 869 This this DT 870 878 adhesion adhesion NN 879 887 molecule molecule NN 888 891 was be VBD 892 896 also also RB 897 906 expressed express VBN 907 909 by by IN 910 911 T t NN 912 917 cells cell NNS 918 926 obtained obtain VBN 927 931 from from IN 932 935 one one CD 936 943 patient patient NN 944 948 with with IN 949 966 HTLV-1-associated htlv-1-associated JJ 967 986 myelopathy/tropical myelopathy/tropical JJ 987 994 spastic spastic JJ 995 1006 paraparesis paraparesis NN 1007 1010 but but CC 1011 1014 not not RB 1015 1017 by by IN 1018 1027 activated activate VBN 1028 1029 T t NN 1030 1035 cells cell NNS 1036 1044 isolated isolate VBN 1045 1049 from from IN 1050 1053 one one CD 1054 1060 normal normal JJ 1061 1066 blood blood NN 1067 1072 donor donor NN 1072 1073 . . . 1074 1077 The the DT 1078 1082 role role NN 1083 1085 of of IN 1086 1089 the the DT 1090 1095 viral viral JJ 1096 1111 trans-activator trans-activator NN 1112 1115 Tax tax NN 1116 1123 protein protein NN 1124 1126 in in IN 1127 1130 the the DT 1131 1140 induction induction NN 1141 1143 of of IN 1144 1150 VCAM-1 VCAM-1 NNP 1151 1154 was be VBD 1155 1160 first first RB 1161 1170 indicated indicate VBN 1171 1173 by by IN 1174 1177 the the DT 1178 1187 detection detection NN 1188 1190 of of IN 1191 1195 this this DT 1196 1204 adhesion adhesion NN 1205 1213 molecule molecule NN 1214 1216 on on IN 1217 1223 Jurkat Jurkat NNP 1224 1230 T-cell T-cell NNP 1231 1237 clones clone NNS 1238 1244 stably stably RB 1245 1255 expressing express VBG 1256 1259 the the DT 1260 1263 tax tax NN 1264 1268 gene gene NN 1268 1269 . . . 1270 1273 The the DT 1274 1280 effect effect NN 1281 1283 of of IN 1284 1287 Tax Tax NNP 1288 1290 on on IN 1291 1297 VCAM-1 vcam-1 NN 1298 1302 gene gene NN 1303 1316 transcription transcription NN 1317 1320 was be VBD 1321 1325 next next RB 1326 1335 confirmed confirm VBN 1336 1338 in in IN 1339 1344 JPX-9 JPX-9 NNP 1345 1350 cells cell NNS 1350 1351 , , , 1352 1353 a a DT 1354 1362 subclone subclone NN 1363 1365 of of IN 1366 1372 Jurkat Jurkat NNP 1373 1378 cells cell NNS 1378 1379 , , , 1380 1388 carrying carry VBG 1389 1392 the the DT 1393 1396 tax tax NN 1397 1406 sequences sequence NNS 1407 1412 under under IN 1413 1416 the the DT 1417 1424 control control NN 1425 1427 of of IN 1428 1430 an an DT 1431 1440 inducible inducible JJ 1441 1449 promoter promoter NN 1449 1450 . . . 1451 1462 Furthermore furthermore RB 1462 1463 , , , 1464 1472 deletion deletion NN 1473 1476 and and CC 1477 1485 mutation mutation NN 1486 1494 analyses analysis NNS 1495 1497 of of IN 1498 1501 the the DT 1502 1508 VCAM-1 vcam-1 NN 1509 1517 promoter promoter NN 1518 1527 performed perform VBN 1528 1532 with with IN 1533 1548 chloramphenicol chloramphenicol NN 1549 1566 acetyltransferase acetyltransferase NN 1567 1577 constructs construct NNS 1578 1586 revealed reveal VBD 1587 1591 that that IN 1592 1595 Tax Tax NNP 1596 1599 was be VBD 1600 1605 trans tran NNS 1606 1616 activating activate VBG 1617 1620 the the DT 1621 1627 VCAM-1 vcam-1 NN 1628 1636 promoter promoter NN 1637 1640 via via IN 1641 1644 two two CD 1645 1654 NF-kappaB NF-kappaB NNP 1655 1660 sites site NNS 1661 1668 present present JJ 1669 1671 at at IN 1672 1674 bp bp NN 1675 1678 -72 -72 CD 1679 1682 and and CC 1683 1686 -57 -57 CD 1687 1689 in in IN 1690 1693 the the DT 1694 1700 VCAM-1 vcam-1 NN 1701 1705 gene gene NN 1706 1714 promoter promoter NN 1714 1715 , , , 1716 1720 with with IN 1721 1725 both both DT 1726 1728 of of IN 1729 1733 them them PRP 1734 1739 being be VBG 1740 1748 required require VBN 1749 1752 for for IN 1753 1756 the the DT 1757 1768 Tax-induced tax-induced JJ 1769 1779 expression expression NN 1780 1782 of of IN 1783 1787 this this DT 1788 1796 adhesion adhesion NN 1797 1805 molecule molecule NN 1805 1806 . . . 1807 1814 Finally finally RB 1814 1815 , , , 1816 1819 gel gel NN 1820 1828 mobility mobility NN 1829 1834 shift shift NN 1835 1841 assays assay NNS 1842 1854 demonstrated demonstrate VBD 1855 1858 the the DT 1859 1866 nuclear nuclear JJ 1867 1880 translocation translocation NN 1881 1883 of of IN 1884 1892 proteins protein NNS 1893 1905 specifically specifically RB 1906 1911 bound bind VBD 1912 1914 to to TO 1915 1920 these these DT 1921 1924 two two CD 1925 1934 NF-kappaB NF-kappaB NNP 1935 1941 motifs motif NNS 1941 1942 , , , 1943 1953 confirming confirm VBG 1954 1958 that that IN 1959 1965 VCAM-1 vcam-1 NN 1966 1969 was be VBD 1970 1977 induced induce VBN 1978 1980 on on IN 1981 1995 Tax-expressing tax-expressing JJ 1996 2001 cells cell NNS 2002 2004 in in IN 2005 2006 a a DT 2007 2023 kappaB-dependent kappab-dependent JJ 2024 2030 manner manner NN 2030 2031 . . . 2032 2044 Collectively collectively RB 2044 2045 , , , 2046 2051 these these DT 2052 2059 results result NNS 2060 2069 therefore therefore RB 2070 2077 suggest suggest VBP 2078 2082 that that IN 2083 2086 the the DT 2087 2096 exclusive exclusive JJ 2097 2108 Tax-induced tax-induced JJ 2109 2119 expression expression NN 2120 2122 of of IN 2123 2129 VCAM-1 vcam-1 NN 2130 2132 on on IN 2133 2134 T t NN 2135 2140 cells cell NNS 2141 2144 may may MD 2145 2154 represent represent VB 2155 2156 a a DT 2157 2164 pivotal pivotal JJ 2165 2170 event event NN 2171 2173 in in IN 2174 2177 the the DT 2178 2189 progression progression NN 2190 2192 of of IN 2193 2210 HTLV-1-associated htlv-1-associated JJ 2211 2219 diseases disease NNS 2219 2220 . . .