0	11	Suppression	suppression	NN
12	14	of	of	IN
15	22	nuclear	nuclear	JJ
23	29	factor	factor	NN
30	35	kappa	kappa	NN
36	37	B	B	NNP
38	41	and	and	CC
42	55	CD18-mediated	cd18-mediated	JJ
56	65	leukocyte	leukocyte	NN
66	74	adhesion	adhesion	NN
75	77	to	to	TO
78	81	the	the	DT
82	89	corneal	corneal	JJ
90	101	endothelium	endothelium	NN
102	104	by	by	IN
105	118	dexamethasone	dexamethasone	NN
118	119	.	.	.

121	128	PURPOSE	purpose	NN
128	129	:	:	:
130	132	To	to	TO
133	144	demonstrate	demonstrate	VB
145	149	that	that	IN
150	159	leukocyte	leukocyte	NN
160	168	adhesion	adhesion	NN
169	171	to	to	TO
172	180	cultured	culture	VBN
181	188	corneal	corneal	NN
189	200	endothelial	endothelial	JJ
201	206	cells	cell	NNS
207	209	is	be	VBZ
210	218	mediated	mediate	VBN
219	221	by	by	IN
222	225	the	the	DT
226	230	CD18	cd18	NN
231	238	antigen	antigen	NN
238	239	,	,	,
240	243	and	and	CC
244	246	to	to	TO
247	256	determine	determine	VB
257	264	whether	whether	IN
265	278	dexamethasone	dexamethasone	NN
279	287	directly	directly	RB
288	298	suppresses	suppress	VBZ
299	307	adhesion	adhesion	NN
308	310	by	by	IN
311	321	inhibiting	inhibit	VBG
322	332	activation	activation	NN
333	335	of	of	IN
336	343	nuclear	nuclear	JJ
344	350	factor	factor	NN
351	356	kappa	kappa	NN
357	358	B	B	NNP
359	360	(	(	(
360	368	NFkappaB	NFkappaB	NNP
368	369	)	)	)
369	370	.	.	.

371	378	METHODS	methods	NNS
378	379	:	:	:
380	388	Cultured	culture	VBN
389	395	bovine	bovine	JJ
396	403	corneal	corneal	NN
404	415	endothelium	endothelium	NN
416	419	was	be	VBD
420	430	stimulated	stimulate	VBN
431	434	for	for	IN
435	436	6	6	CD
437	442	hours	hour	NNS
443	445	by	by	IN
446	448	40	40	CD
449	458	micron/ml	micron/ml	NN
459	464	tumor	tumor	NN
465	473	necrosis	necrosis	NN
474	480	factor	factor	NN
481	486	alpha	alpha	NN
487	488	(	(	(
488	496	TNFalpha	TNFalpha	NNP
496	497	)	)	)
497	498	.	.	.

499	512	Dexamethasone	Dexamethasone	NNP
513	516	was	be	VBD
517	522	added	add	VBN
523	524	1	1	CD
525	529	hour	hour	NN
530	536	before	before	IN
537	545	TNFalpha	TNFalpha	NNP
546	557	stimulation	stimulation	NN
558	560	in	in	IN
561	564	the	the	DT
565	578	dexamethasone	dexamethasone	NN
579	584	group	group	NN
584	585	.	.	.

586	591	After	after	IN
592	603	stimulation	stimulation	NN
603	604	,	,	,
605	616	neutrophils	neutrophil	NNS
617	626	separated	separate	VBN
627	631	from	from	IN
632	633	a	a	DT
634	641	healthy	healthy	JJ
642	647	human	human	JJ
648	657	volunteer	volunteer	NN
658	662	were	be	VBD
663	668	added	add	VBN
669	673	with	with	IN
674	676	or	or	CC
677	684	without	without	IN
685	694	anti-CD18	anti-cd18	JJ
695	703	antibody	antibody	NN
703	704	.	.	.

705	708	The	the	DT
709	716	culture	culture	NN
717	722	plate	plate	NN
723	726	was	be	VBD
727	734	settled	settle	VBN
735	738	for	for	IN
739	741	15	15	CD
742	749	minutes	minute	NNS
750	752	at	at	IN
753	755	37	37	CD
756	763	degrees	degree	NNS
764	765	C	C	NNP
765	766	,	,	,
767	770	and	and	CC
771	775	then	then	RB
776	787	neutrophils	neutrophil	NNS
788	792	were	be	VBD
793	802	activated	activate	VBN
803	805	by	by	IN
806	845	N-formyl-methionyl-leucyl-phenylalanine	N-formyl-methionyl-leucyl-phenylalanine	NNP
846	849	for	for	IN
850	851	5	5	CD
852	859	minutes	minute	NNS
859	860	.	.	.

861	872	Nonadherent	nonadherent	JJ
873	884	neutrophils	neutrophil	NNS
885	889	were	be	VBD
890	897	removed	remove	VBN
898	900	by	by	IN
901	908	sealing	seal	VBG
909	912	and	and	CC
913	922	inverting	invert	VBG
923	926	the	the	DT
927	934	culture	culture	NN
935	939	well	well	RB
939	940	.	.	.

941	944	The	the	DT
945	958	intracellular	intracellular	JJ
959	971	localization	localization	NN
972	974	of	of	IN
975	983	NFkappaB	NFkappaB	NNP
984	989	after	after	IN
990	998	TNFalpha	TNFalpha	NNP
999	1009	simulation	simulation	NN
1010	1013	was	be	VBD
1014	1024	determined	determine	VBN
1025	1027	by	by	IN
1028	1036	confocal	confocal	JJ
1037	1056	immunocytochemistry	immunocytochemistry	NN
1057	1062	using	use	VBG
1063	1065	an	an	DT
1066	1074	anti-p65	anti-p65	JJ
1075	1083	antibody	antibody	NN
1083	1084	.	.	.

1085	1092	RESULTS	results	NNS
1092	1093	:	:	:
1094	1104	Neutrophil	Neutrophil	NNP
1105	1113	adhesion	adhesion	NN
1114	1116	to	to	TO
1117	1125	cultured	culture	VBN
1126	1133	corneal	corneal	NN
1134	1145	endothelial	endothelial	JJ
1146	1151	cells	cell	NNS
1152	1161	increased	increase	VBD
1162	1175	significantly	significantly	RB
1176	1178	on	on	IN
1179	1187	exposure	exposure	NN
1188	1190	to	to	TO
1191	1199	TNFalpha	TNFalpha	NNP
1200	1201	(	(	(
1201	1213	451.4+/-45.4	451.4+/-45.4	CD
1214	1223	cells/mm2	cells/mm2	NNS
1223	1224	,	,	,
1225	1226	n	n	NN
1227	1228	=	=	JJ
1229	1231	16	16	CD
1231	1232	)	)	)
1233	1241	compared	compare	VBN
1242	1244	to	to	TO
1245	1252	control	control	NN
1253	1254	(	(	(
1254	1266	156.7+/-27.3	156.7+/-27.3	CD
1267	1276	cells/mm2	cells/mm2	NNS
1276	1277	,	,	,
1278	1279	n	n	NN
1280	1281	=	=	SYM
1282	1284	16	16	CD
1284	1285	,	,	,
1286	1287	P	p	NN
1288	1289	<	<	SYM
1290	1294	0.01	0.01	CD
1294	1295	)	)	)
1295	1296	.	.	.

1297	1301	This	this	DT
1302	1311	increased	increase	VBN
1312	1320	adhesion	adhesion	NN
1321	1324	was	be	VBD
1325	1335	suppressed	suppress	VBN
1336	1338	by	by	IN
1339	1342	the	the	DT
1343	1351	addition	addition	NN
1352	1354	of	of	IN
1355	1364	anti-CD18	anti-cd18	JJ
1365	1373	antibody	antibody	NN
1374	1375	(	(	(
1375	1387	157.6+/-25.1	157.6+/-25.1	CD
1388	1397	cells/mm2	cells/mm2	NNS
1397	1398	,	,	,
1399	1400	n	n	NN
1401	1402	=	=	SYM
1403	1404	8	8	CD
1404	1405	,	,	,
1406	1407	P	p	NN
1408	1409	<	<	SYM
1410	1414	0.01	0.01	CD
1414	1415	)	)	)
1416	1419	and	and	CC
1420	1422	by	by	IN
1423	1435	pretreatment	pretreatment	NN
1436	1440	with	with	IN
1441	1447	10(-7)	10(-7)	CD
1448	1449	M	M	NNP
1450	1463	dexamethasone	dexamethasone	NN
1464	1465	(	(	(
1465	1477	207.9+/-31.5	207.9+/-31.5	CD
1478	1487	cells/mm2	cells/mm2	NNS
1487	1488	,	,	,
1489	1490	n	n	NN
1491	1492	=	=	SYM
1493	1495	10	10	CD
1495	1496	,	,	,
1497	1498	P	p	NN
1499	1500	<	<	SYM
1501	1505	0.01	0.01	CD
1505	1506	)	)	)
1506	1507	.	.	.

1508	1527	Immunocytochemistry	Immunocytochemistry	NNP
1528	1530	60	60	CD
1531	1538	minutes	minute	NNS
1539	1544	after	after	IN
1545	1556	stimulation	stimulation	NN
1557	1565	revealed	reveal	VBD
1566	1570	that	that	IN
1571	1579	NFkappaB	NFkappaB	NNP
1580	1583	was	be	VBD
1584	1591	located	locate	VBN
1592	1594	in	in	IN
1595	1598	the	the	DT
1599	1608	cytoplasm	cytoplasm	NN
1609	1611	in	in	IN
1612	1624	unstimulated	unstimulated	JJ
1625	1630	cells	cell	NNS
1630	1631	;	;	:
1632	1639	however	however	RB
1639	1640	,	,	,
1641	1644	the	the	DT
1645	1653	addition	addition	NN
1654	1656	of	of	IN
1657	1665	TNFalpha	TNFalpha	NNP
1666	1672	caused	cause	VBD
1673	1681	NFkappaB	NFkappaB	NNP
1682	1684	to	to	TO
1685	1696	translocate	translocate	VB
1697	1701	into	into	IN
1702	1705	the	the	DT
1706	1713	nucleus	nucleus	NN
1713	1714	.	.	.

1715	1727	Pretreatment	Pretreatment	NNP
1728	1732	with	with	IN
1733	1746	dexamethasone	dexamethasone	NN
1747	1754	tapered	taper	VBD
1755	1763	NFkappaB	nfkappab	NN
1764	1777	translocation	translocation	NN
1778	1782	into	into	IN
1783	1786	the	the	DT
1787	1794	nucleus	nucleus	NN
1794	1795	.	.	.

1796	1807	CONCLUSIONS	conclusions	NNS
1807	1808	:	:	:
1809	1818	Leukocyte	Leukocyte	NNP
1819	1827	adhesion	adhesion	NN
1828	1830	to	to	TO
1831	1834	the	the	DT
1835	1842	corneal	corneal	NN
1843	1854	endothelium	endothelium	NN
1855	1858	was	be	VBD
1859	1864	shown	show	VBN
1865	1867	to	to	TO
1868	1870	be	be	VB
1871	1879	mediated	mediate	VBN
1880	1882	by	by	IN
1883	1887	CD18	cd18	NN
1888	1897	expressed	express	VBN
1898	1900	on	on	IN
1901	1910	activated	activate	VBN
1911	1921	leukocytes	leukocyte	NNS
1921	1922	.	.	.

1923	1935	Pretreatment	Pretreatment	NNP
1936	1938	of	of	IN
1939	1942	the	the	DT
1943	1954	endothelium	endothelium	NN
1955	1959	with	with	IN
1960	1973	dexamethasone	dexamethasone	NN
1974	1983	inhibited	inhibit	VBD
1984	1993	leukocyte	leukocyte	NN
1994	2002	adhesion	adhesion	NN
2002	2003	;	;	:
2004	2008	this	this	DT
2009	2012	may	may	MD
2013	2015	be	be	VB
2016	2019	due	due	JJ
2020	2022	in	in	IN
2023	2027	part	part	NN
2028	2030	to	to	TO
2031	2034	the	the	DT
2035	2046	suppression	suppression	NN
2047	2049	of	of	IN
2050	2058	NFkappaB	NFkappaB	NNP
2059	2064	entry	entry	NN
2065	2069	into	into	IN
2070	2073	the	the	DT
2074	2081	nucleus	nucleus	NN
2081	2082	.	.	.