0 9 Molecular molecular JJ 10 17 cloning cloning NN 18 21 and and CC 22 32 functional functional JJ 33 49 characterization characterization NN 50 52 of of IN 53 59 murine murine JJ 60 64 cDNA cdna NN 65 73 encoding encode VBG 74 87 transcription transcription NN 88 94 factor factor NN 95 100 NFATc NFATc NNP 100 101 . . . 103 116 Transcription transcription NN 117 124 factors factor NNS 125 127 of of IN 128 131 the the DT 132 136 NFAT NFAT NNP 137 138 ( ( ( 138 145 nuclear nuclear JJ 146 152 factor factor NN 153 155 of of IN 156 165 activated activate VBN 166 167 T t NN 168 173 cells cell NNS 173 174 ) ) ) 175 181 family family NN 182 186 play play VBP 187 196 important important JJ 197 202 roles role NNS 203 205 in in IN 206 212 immune immune JJ 213 216 and and CC 217 229 inflammatory inflammatory JJ 230 239 responses response NNS 240 242 by by IN 243 253 regulating regulate VBG 254 257 the the DT 258 268 expression expression NN 269 271 of of IN 272 277 genes gene NNS 278 286 encoding encode VBG 287 296 cytokines cytokine NNS 297 300 and and CC 301 317 immunoregulatory immunoregulatory JJ 318 326 proteins protein NNS 326 327 . . . 328 332 Here here RB 333 335 we we PRP 336 344 describe describe VBP 345 352 cloning cloning NN 353 356 and and CC 357 373 characterization characterization NN 374 376 of of IN 377 388 full-length full-length JJ 389 393 cDNA cdna NN 394 402 encoding encode VBG 403 409 murine murine JJ 410 411 ( ( ( 411 412 m m NN 412 413 ) ) ) 414 419 NFATc NFATc NNP 420 425 which which WDT 426 434 predicts predict VBZ 435 439 that that IN 440 443 the the DT 444 451 protein protein NN 452 455 has have VBZ 456 459 all all PDT 460 463 the the DT 464 473 conserved conserve VBN 474 484 structural structural JJ 485 491 motifs motif NNS 492 494 of of IN 495 499 NFAT NFAT NNP 500 506 family family NN 507 514 members member NNS 514 515 , , , 516 525 including include VBG 526 529 the the DT 530 533 rel rel NN 534 542 homology homology NN 543 549 domain domain NN 549 550 , , , 551 554 the the DT 555 559 NFAT NFAT NNP 560 568 homology homology NN 569 575 domain domain NN 576 579 and and CC 580 583 the the DT 584 591 nuclear nuclear JJ 592 605 translocation translocation NN 606 614 signals. signals. NN 615 621 mNFATc mnfatc NN 622 631 complexed complexe VBN 632 636 with with IN 637 641 AP-1 ap-1 NN 642 647 bound bind VBD 648 660 specifically specifically RB 661 663 to to TO 664 667 the the DT 668 674 murine murine JJ 675 679 IL-2 il-2 NN 680 684 NFAT NFAT NNP 685 696 recognition recognition NN 697 705 sequence sequence NN 706 709 and and CC 710 719 activated activate VBD 720 733 transcription transcription NN 734 738 from from IN 739 742 the the DT 743 757 co-transfected co-transfected JJ 758 762 IL-2 il-2 NN 763 771 promoter promoter NN 772 774 in in IN 775 780 COS-7 cos-7 NN 781 786 cells cell NNS 786 787 . . . 788 796 Northern Northern NNP 797 801 blot blot NN 802 810 analysis analysis NN 811 817 showed show VBD 818 822 that that IN 823 826 the the DT 827 831 cDNA cdna NN 832 837 probe probe NN 838 848 hybridized hybridize VBN 849 853 with with IN 854 855 a a DT 856 859 4.5 4.5 CD 860 862 kb kb NN 863 873 transcript transcript NN 874 879 which which WDT 880 882 is be VBZ 883 889 highly highly RB 890 899 inducible inducible JJ 900 902 in in IN 903 909 murine murine JJ 910 911 T t NN 912 917 cells cell NNS 917 918 . . . 919 921 By by IN 922 930 Northern Northern NNP 931 934 and and CC 935 937 in in FW 938 942 situ situ FW 943 956 hybridization hybridization NN 956 957 , , , 958 964 mNFATc mnfatc NN 965 975 transcript transcript NN 976 979 was be VBD 980 988 detected detect VBN 989 993 from from IN 994 997 the the DT 998 1003 early early JJ 1004 1009 stage stage NN 1010 1012 of of IN 1013 1024 development development NN 1024 1025 . . . 1026 1028 In in IN 1029 1032 the the DT 1033 1038 mouse mouse NN 1039 1045 embryo embryo NN 1045 1046 , , , 1047 1053 mNFATc mnfatc NN 1054 1064 transcript transcript NN 1065 1068 was be VBD 1069 1077 strongly strongly RB 1078 1087 expressed express VBN 1088 1090 in in IN 1091 1097 thymus thymus NN 1097 1098 , , , 1099 1103 lung lung NN 1104 1107 and and CC 1108 1121 submandibular submandibular JJ 1122 1127 gland gland NN 1128 1131 and and CC 1132 1138 weakly weakly RB 1139 1141 in in IN 1142 1150 skeletal skeletal JJ 1151 1157 muscle muscle NN 1158 1161 and and CC 1162 1167 heart heart NN 1168 1178 suggesting suggest VBG 1179 1183 that that IN 1184 1190 mNFATc mnfatc NN 1191 1194 may may MD 1195 1199 have have VB 1200 1201 a a DT 1202 1206 role role NN 1207 1211 both both CC 1212 1214 in in IN 1215 1228 embryogenesis embryogenesis NN 1229 1232 and and CC 1233 1235 in in IN 1236 1242 mature mature JJ 1243 1244 T t NN 1245 1250 cells cell NNS 1250 1251 . . .