0 4 IL-2 IL-2 NNP 5 8 and and CC 9 13 IL-7 IL-7 NNP 14 20 induce induce VBP 21 39 heterodimerization heterodimerization NN 40 42 of of IN 43 48 STAT5 STAT5 NNP 49 57 isoforms isoform NNS 58 60 in in IN 61 66 human human JJ 67 77 peripheral peripheral JJ 78 83 blood blood NN 84 85 T t NN 86 98 lymphoblasts lymphoblast NNS 98 99 . . . 101 108 Despite despite IN 109 120 differences difference NNS 121 123 in in IN 124 125 T t NN 126 130 cell cell NN 131 140 responses response NNS 141 148 induced induce VBN 149 151 by by IN 152 163 interleukin interleukin NN 164 165 ( ( ( 165 170 IL)-2 il)-2 NN 171 174 and and CC 175 179 IL-7 IL-7 NNP 179 180 , , , 181 185 both both DT 186 195 cytokines cytokine NNS 196 204 modulate modulate VBP 205 206 T t NN 207 211 cell cell NN 212 221 functions function NNS 222 224 by by IN 225 235 activation activation NN 236 238 of of IN 239 245 signal signal NN 246 257 transducers transducer NNS 258 261 and and CC 262 272 activators activator NNS 273 275 of of IN 276 289 transcription transcription NN 290 291 ( ( ( 291 295 STAT STAT NNP 295 296 ) ) ) 297 305 proteins protein NNS 305 306 . . . 307 309 We we PRP 310 318 examined examine VBD 319 322 the the DT 323 335 contribution contribution NN 336 338 of of IN 339 342 the the DT 343 346 two two CD 347 355 isoforms isoform NNS 356 358 of of IN 359 364 STAT5 STAT5 NNP 364 365 , , , 366 372 STAT5A STAT5A NNP 373 376 and and CC 377 383 STAT5B STAT5B NNP 383 384 , , , 385 387 to to TO 388 393 IL-2- il-2- NN 394 397 and and CC 398 410 IL-7-induced il-7-induced JJ 411 421 activation activation NN 422 424 of of IN 425 430 human human JJ 431 441 peripheral peripheral JJ 442 447 blood blood NN 448 449 T t NN 450 462 lymphoblasts lymphoblast NNS 462 463 . . . 464 468 Both both DT 469 478 cytokines cytokine NNS 479 486 induced induce VBD 487 495 assembly assembly NN 496 498 of of IN 499 505 STAT5A STAT5A NNP 506 509 and and CC 510 516 STAT5B STAT5B NNP 517 527 containing contain VBG 528 537 complexes complex NNS 538 545 capable capable JJ 546 548 of of IN 549 556 binding bind VBG 557 559 to to TO 560 563 the the DT 564 580 interferon-gamma interferon-gamma NN 581 591 activation activation NN 592 600 sequence sequence NN 601 602 ( ( ( 602 605 GAS GAS NNP 605 606 ) ) ) 606 607 , , , 608 611 and and CC 612 617 these these DT 618 627 complexes complex NNS 628 635 rapidly rapidly RB 636 648 translocated translocate VBD 649 650 ( ( ( 650 656 within within IN 657 658 1 1 CD 659 662 min min NN 662 663 ) ) ) 664 668 into into IN 669 672 the the DT 673 680 nucleus nucleus NN 681 683 of of IN 684 689 IL-2- il-2- NN 690 692 or or CC 693 705 IL-7-treated il-7-treated JJ 706 711 cells cell NNS 711 712 . . . 713 716 The the DT 717 725 kinetics kinetic NNS 726 728 of of IN 729 733 this this DT 734 747 translocation translocation NN 748 752 were be VBD 753 760 delayed delay VBN 761 763 in in IN 764 776 IL-7-treated il-7-treated JJ 777 779 as as IN 780 788 compared compare VBN 789 791 to to TO 792 804 IL-2-treated il-2-treated JJ 805 810 cells cell NNS 810 811 . . . 812 816 IL-2 IL-2 NNP 817 820 and and CC 821 825 IL-7 IL-7 NNP 826 830 were be VBD 831 841 equivalent equivalent JJ 842 844 in in IN 845 850 their their PRP$ 851 858 ability ability NN 859 861 to to TO 862 868 induce induce VB 869 877 tyrosine tyrosine NN 878 893 phosphorylation phosphorylation NN 894 896 of of IN 897 903 STAT5A STAT5A NNP 904 907 and and CC 908 914 STAT5B STAT5B NNP 915 918 and and CC 919 921 to to TO 922 932 facilitate facilitate VB 933 940 binding binding NN 941 943 of of IN 944 949 these these DT 950 955 STATs stat NNS 956 958 to to TO 959 961 an an DT 962 973 immobilized immobilize VBN 974 977 GAS GAS NNP 978 985 element element NN 985 986 . . . 987 991 Both both CC 992 996 IL-2 il-2 NN 997 1000 and and CC 1001 1005 IL-7 IL-7 NNP 1006 1013 induced induce VBD 1014 1025 substantial substantial JJ 1026 1033 amounts amount NNS 1034 1036 of of IN 1037 1050 STAT5A/STAT5B STAT5A/STAT5B NNP 1051 1069 heterodimerization heterodimerization NN 1069 1070 . . . 1071 1079 Moreover moreover RB 1079 1080 , , , 1081 1083 we we PRP 1084 1092 observed observe VBD 1093 1105 constitutive constitutive JJ 1106 1117 association association NN 1118 1120 of of IN 1121 1126 STAT3 stat3 NN 1127 1131 with with IN 1132 1136 each each DT 1137 1142 STAT5 stat5 NN 1143 1149 isomer isomer NN 1149 1150 . . . 1151 1156 These these DT 1157 1161 data datum NNS 1162 1169 suggest suggest VBP 1170 1174 that that IN 1175 1179 IL-2 il-2 NN 1180 1183 and and CC 1184 1188 IL-7 IL-7 NNP 1189 1195 induce induce VBP 1196 1204 assembly assembly NN 1205 1207 of of IN 1208 1212 STAT stat NN 1213 1225 heterodimers heterodimer NNS 1226 1228 in in IN 1229 1230 a a DT 1231 1238 similar similar JJ 1239 1245 manner manner NN 1246 1249 and and CC 1250 1254 that that IN 1255 1265 subsequent subsequent JJ 1266 1274 cellular cellular JJ 1275 1284 responses response NNS 1285 1288 may may MD 1289 1291 be be VB 1292 1298 driven drive VBN 1299 1301 by by IN 1302 1311 induction induction NN 1312 1314 of of IN 1315 1322 similar similar JJ 1323 1327 sets set NNS 1328 1330 of of IN 1331 1336 genes gene NNS 1336 1337 . . .