0 9 Molecular molecular JJ 10 20 mechanisms mechanism NNS 21 23 of of IN 24 52 anoxia/reoxygenation-induced anoxia/reoxygenation-induced JJ 53 63 neutrophil neutrophil NN 64 73 adherence adherence NN 74 76 to to TO 77 85 cultured culture VBN 86 97 endothelial endothelial JJ 98 103 cells cell NNS 103 104 . . . 106 109 The the DT 110 120 objectives objective NNS 121 123 of of IN 124 128 this this DT 129 134 study study NN 135 139 were be VBD 140 142 to to TO 143 144 ( ( ( 144 145 1 1 CD 145 146 ) ) ) 147 156 determine determine VBP 157 160 the the DT 161 165 time time NN 166 172 course course NN 173 175 of of IN 176 186 neutrophil neutrophil NN 187 195 adhesion adhesion NN 196 198 to to TO 199 209 monolayers monolayer NNS 210 212 of of IN 213 218 human human JJ 219 228 umbilical umbilical JJ 229 233 vein vein NN 234 245 endothelial endothelial JJ 246 251 cells cell NNS 252 253 ( ( ( 253 259 HUVECs HUVEC NNP 259 260 ) ) ) 261 265 that that WDT 266 270 were be VBD 271 278 exposed expose VBN 279 281 to to TO 282 284 60 60 CD 285 292 minutes minute NNS 293 295 of of IN 296 302 anoxia anoxia NN 303 311 followed follow VBN 312 314 by by IN 315 317 30 30 CD 318 320 to to TO 321 324 600 600 CD 325 332 minutes minute NNS 333 335 of of IN 336 349 reoxygenation reoxygenation NN 350 353 and and CC 354 355 ( ( ( 355 356 2 2 LS 356 357 ) ) ) 358 364 define define VB 365 368 the the DT 369 379 mechanisms mechanism NNS 380 391 responsible responsible JJ 392 395 for for IN 396 400 both both CC 401 404 the the DT 405 410 early early JJ 411 412 ( ( ( 412 419 minutes minute NNS 419 420 ) ) ) 421 424 and and CC 425 429 late late JJ 430 431 ( ( ( 431 436 hours hour NNS 436 437 ) ) ) 438 453 hyperadhesivity hyperadhesivity NN 454 456 of of IN 457 467 postanoxic postanoxic JJ 468 474 HUVECs huvec NNS 475 477 to to TO 478 483 human human JJ 484 495 neutrophils neutrophil NNS 495 496 . . . 497 500 The the DT 501 508 results result NNS 509 516 clearly clearly RB 517 528 demonstrate demonstrate VBP 529 533 that that IN 534 554 anoxia/reoxygenation anoxia/reoxygenation NN 555 556 ( ( ( 556 559 A/R A/R NNP 559 560 ) ) ) 561 566 leads lead VBZ 567 569 to to TO 570 571 a a DT 572 580 biphasic biphasic JJ 581 589 increase increase NN 590 592 in in IN 593 603 neutrophil neutrophil NN 604 612 adhesion adhesion NN 613 615 to to TO 616 622 HUVECs HUVEC NNP 622 623 , , , 624 628 with with IN 629 633 peak peak NN 634 643 responses response NNS 644 653 occurring occur VBG 654 656 at at IN 657 659 30 30 CD 660 667 minutes minute NNS 668 669 ( ( ( 669 674 phase phase NN 675 676 1 1 CD 676 677 ) ) ) 678 681 and and CC 682 685 240 240 CD 686 693 minutes minute NNS 694 695 ( ( ( 695 700 phase phase NN 701 702 2 2 CD 702 703 ) ) ) 704 709 after after IN 710 723 reoxygenation reoxygenation NN 723 724 . . . 725 735 Oxypurinol Oxypurinol NNP 736 739 and and CC 740 748 catalase catalase NN 749 758 inhibited inhibit VBD 759 766 phase-1 phase-1 NN 767 775 adhesion adhesion NN 775 776 , , , 777 787 suggesting suggest VBG 788 789 a a DT 790 794 role role NN 795 798 for for IN 799 807 xanthine xanthine NN 808 815 oxidase oxidase NN 816 819 and and CC 820 824 H2O2 h2o2 NN 824 825 . . . 826 828 In in IN 829 839 comparison comparison NN 839 840 , , , 841 849 platelet platelet NN 850 860 activating activating NN 861 867 factor factor NN 868 869 ( ( ( 869 872 PAF PAF NNP 872 873 ) ) ) 874 885 contributed contribute VBD 886 888 to to TO 889 893 both both DT 894 900 phases phase NNS 901 903 of of IN 904 914 neutrophil neutrophil NN 915 923 adhesion adhesion NN 923 924 . . . 925 943 Anti-intercellular anti-intercellular JJ 944 952 adhesion adhesion NN 953 963 molecule-1 molecule-1 NN 964 965 ( ( ( 965 971 ICAM-1 icam-1 NN 971 972 ) ) ) 973 976 and and CC 977 992 anti-P-selectin anti-p-selectin JJ 993 1003 antibodies antibody NNS 1004 1005 ( ( ( 1005 1015 monoclonal monoclonal JJ 1016 1026 antibodies antibody NNS 1027 1028 [ [ ( 1028 1032 mAbs mab NNS 1032 1033 ] ] ) 1033 1034 ) ) ) 1035 1045 attenuated attenuate VBD 1046 1053 phase-1 phase-1 JJ 1054 1064 neutrophil neutrophil NN 1065 1073 adhesion adhesion NN 1073 1074 , , , 1075 1085 consistent consistent JJ 1086 1090 with with IN 1091 1096 roles role NNS 1097 1100 for for IN 1101 1115 constitutively constitutively RB 1116 1125 expressed express VBN 1126 1132 ICAM-1 icam-1 NN 1133 1136 and and CC 1137 1145 enhanced enhance VBN 1146 1153 surface surface NN 1154 1164 expression expression NN 1165 1167 of of IN 1168 1177 preformed preformed JJ 1178 1188 P-selectin P-selectin NNP 1188 1189 . . . 1190 1197 Phase-2 phase-2 NN 1198 1208 neutrophil neutrophil NN 1209 1217 adhesion adhesion NN 1218 1221 was be VBD 1222 1232 attenuated attenuate VBN 1233 1235 by by IN 1236 1238 an an DT 1239 1254 anti-E-selectin anti-e-selectin JJ 1255 1258 mAb mab NN 1258 1259 , , , 1260 1270 indicating indicate VBG 1271 1272 a a DT 1273 1281 dominant dominant JJ 1282 1286 role role NN 1287 1289 of of IN 1290 1294 this this DT 1295 1303 adhesion adhesion NN 1304 1312 molecule molecule NN 1313 1315 in in IN 1316 1319 the the DT 1320 1324 late late JJ 1325 1330 phase phase NN 1331 1339 response response NN 1339 1340 . . . 1341 1353 Pretreatment Pretreatment NNP 1354 1358 with with IN 1359 1370 actinomycin actinomycin NN 1371 1372 D D NNP 1373 1376 and and CC 1377 1390 cycloheximide cycloheximide NN 1391 1393 or or CC 1394 1398 with with IN 1399 1408 competing compete VBG 1409 1428 ds-oligonucleotides ds-oligonucleotide NNS 1429 1439 containing contain VBG 1440 1443 the the DT 1444 1451 nuclear nuclear JJ 1452 1464 factor-kappa factor-kappa NN 1465 1466 B b NN 1467 1469 or or CC 1470 1479 activator activator NN 1480 1489 protein-1 protein-1 NN 1490 1497 cognate cognate NN 1498 1501 DNA dna NN 1502 1511 sequences sequence NNS 1512 1525 significantly significantly RB 1526 1536 attenuated attenuate VBD 1537 1544 phase-2 phase-2 NN 1545 1553 response response NN 1553 1554 , , , 1555 1565 suggesting suggest VBG 1566 1567 a a DT 1568 1572 role role NN 1573 1576 for for IN 1577 1579 de de FW 1580 1584 novo novo FW 1585 1598 macromolecule macromolecule NN 1599 1608 synthesis synthesis NN 1608 1609 . . . 1610 1617 Surface surface NN 1618 1628 expression expression NN 1629 1631 of of IN 1632 1638 ICAM-1 ICAM-1 NNP 1638 1639 , , , 1640 1650 P-selectin P-selectin NNP 1650 1651 , , , 1652 1655 and and CC 1656 1666 E-selectin E-selectin NNP 1667 1669 on on IN 1670 1676 HUVECs HUVEC NNP 1677 1687 correlated correlate VBD 1688 1692 with with IN 1693 1696 the the DT 1697 1704 phase-1 phase-1 NN 1705 1708 and and CC 1709 1711 -2 -2 CD 1712 1722 neutrophil neutrophil NN 1723 1731 adhesion adhesion NN 1732 1741 responses response NNS 1741 1742 . . . 1743 1755 Collectively collectively RB 1755 1756 , , , 1757 1762 these these DT 1763 1771 findings finding NNS 1772 1780 indicate indicate VBP 1781 1785 that that IN 1786 1789 A/R A/R NNP 1790 1797 elicits elicit VBZ 1798 1799 a a DT 1800 1809 two-phase two-phase JJ 1810 1832 neutrophil-endothelial neutrophil-endothelial JJ 1833 1837 cell cell NN 1838 1846 adhesion adhesion NN 1847 1855 response response NN 1856 1860 that that WDT 1861 1869 involves involve VBZ 1870 1895 transcription-independent transcription-independent JJ 1896 1899 and and CC 1900 1923 transcription-dependent transcription-dependent JJ 1924 1931 surface surface NN 1932 1942 expression expression NN 1943 1945 of of IN 1946 1955 different different JJ 1956 1967 endothelial endothelial JJ 1968 1972 cell cell NN 1973 1981 adhesion adhesion NN 1982 1991 molecules molecule NNS 1991 1992 . . .