0 1 c c SYM 1 2 - - : 2 5 Rel Rel NNP 6 9 and and CC 10 13 p65 p65 NN 14 22 subunits subunit NNS 23 27 bind bind VBP 28 30 to to TO 31 33 an an DT 34 42 upstream upstream JJ 43 52 NF-kappaB nf-kappab NN 53 57 site site NN 58 60 in in IN 61 66 human human JJ 67 78 granulocyte granulocyte NN 79 96 macrophage-colony macrophage-colony NN 97 108 stimulating stimulate VBG 109 115 factor factor NN 116 124 promoter promoter NN 125 133 involved involve VBN 134 136 in in IN 137 144 phorbol phorbol NN 145 150 ester ester NN 151 159 response response NN 160 162 in in IN 163 167 5637 5637 CD 168 173 cells cell NNS 173 174 . . . 176 178 To to TO 179 186 further further RB 187 194 clarify clarify VB 195 198 the the DT 199 206 complex complex JJ 207 222 transcriptional transcriptional JJ 223 233 regulation regulation NN 234 236 of of IN 237 240 the the DT 241 246 human human JJ 247 253 GM-CSF GM-CSF NNP 254 258 gene gene NN 258 259 , , , 260 265 which which WDT 266 269 was be VBD 270 281 extensively extensively RB 282 294 investigated investigate VBN 295 297 in in IN 298 307 activated activate VBN 308 309 T t NN 310 315 cells cell NNS 315 316 , , , 317 319 we we PRP 320 324 have have VBP 325 332 studied study VBN 333 336 the the DT 337 341 role role NN 342 344 of of IN 345 347 an an DT 348 356 upstream upstream JJ 357 366 NF-kappaB nf-kappab NN 367 371 like like JJ 372 376 site site NN 377 379 in in IN 380 383 the the DT 384 388 5637 5637 CD 389 401 non-lymphoid non-lymphoid JJ 402 406 cell cell NN 407 411 line line NN 411 412 , , , 413 418 which which WDT 419 426 derives derive VBZ 427 431 from from IN 432 433 a a DT 434 441 bladder bladder NN 442 451 carcinoma carcinoma NN 452 455 and and CC 456 470 constitutively constitutively RB 471 479 produces produce VBZ 480 486 GM-CSF GM-CSF NNP 486 487 . . . 488 492 This this DT 493 501 sequence sequence NN 501 502 , , , 503 508 named name VBD 509 512 the the DT 513 514 A a NN 515 522 element element NN 522 523 , , , 524 527 has have VBZ 528 530 an an DT 531 537 active active JJ 538 542 role role NN 543 545 on on IN 546 552 GM-CSF GM-CSF NNP 553 566 transcription transcription NN 567 570 and and CC 571 573 is be VBZ 574 584 responsive responsive JJ 585 587 to to TO 588 591 the the DT 592 597 tumor tumor NN 598 606 promoter promoter NN 607 610 PMA PMA NNP 611 613 in in IN 614 623 transient transient JJ 624 636 transfection transfection NN 637 648 experiments experiment NNS 648 649 . . . 650 652 We we PRP 653 661 describe describe VBP 662 666 here here RB 667 668 a a DT 669 682 heterodimeric heterodimeric JJ 683 690 binding binding NN 691 698 complex complex NN 699 701 of of IN 702 711 NF-kappaB NF-kappaB NNP 712 720 subunits subunit NNS 721 722 ( ( ( 722 727 c-Rel c-rel NN 728 731 and and CC 732 735 p65 p65 NN 735 736 ) ) ) 737 742 which which WDT 743 745 is be VBZ 746 755 identical identical JJ 756 758 to to TO 759 762 the the DT 763 766 one one CD 767 775 obtained obtain VBN 776 781 using use VBG 782 785 the the DT 786 800 HIV-LTR-kappaB HIV-LTR-kappaB NNP 801 805 site site NN 806 808 as as IN 809 820 recognition recognition NN 821 829 sequence sequence NN 830 833 and and CC 834 843 different different JJ 844 848 from from IN 849 852 the the DT 853 856 one one CD 857 858 ( ( ( 858 863 c-Rel c-rel NN 864 867 and and CC 868 871 p50 p50 NN 871 872 ) ) ) 873 881 observed observe VBN 882 886 with with IN 887 894 nuclear nuclear JJ 895 903 extracts extract NNS 904 908 from from IN 909 911 Mo Mo NNP 912 922 T-lymphoid t-lymphoid JJ 923 930 HTLV-II HTLV-II NNP 931 939 infected infected JJ 940 945 cells cell NNS 945 946 . . .