0 8 Estrogen Estrogen NNP 9 17 receptor receptor NN 18 28 diminishes diminish VBZ 29 40 DNA-binding dna-binding JJ 41 51 activities activity NNS 52 54 of of IN 55 62 chicken chicken NN 63 69 GATA-1 gata-1 NN 70 73 and and CC 74 87 CACCC-binding caccc-binding JJ 88 96 proteins protein NNS 96 97 . . . 99 102 The the DT 103 111 estrogen estrogen NN 112 120 receptor receptor NN 121 122 ( ( ( 122 124 ER ER NNP 124 125 ) ) ) 126 135 repressed repress VBD 136 145 erythroid erythroid JJ 146 161 differentiation differentiation NN 162 165 and and CC 166 184 erythroid-specific erythroid-specific JJ 185 189 gene gene NN 190 200 expression expression NN 200 201 . . . 202 204 In in IN 205 209 this this DT 210 215 study study NN 215 216 , , , 217 219 we we PRP 220 232 investigated investigate VBD 233 236 the the DT 237 243 effect effect NN 244 246 of of IN 247 249 ER ER NNP 250 255 alpha alpha NN 256 257 ( ( ( 257 265 referred refer VBN 266 268 to to TO 269 279 throughout throughout IN 280 282 as as IN 283 285 ER ER NNP 285 286 ) ) ) 287 289 on on IN 290 301 DNA-binding dna-binding JJ 302 312 activities activity NNS 313 315 of of IN 316 329 transcription transcription NN 330 337 factors factor NNS 338 346 involved involve VBN 347 349 in in IN 350 360 regulating regulate VBG 361 364 the the DT 365 375 expression expression NN 376 378 of of IN 379 397 erythroid-specific erythroid-specific JJ 398 403 genes gene NNS 403 404 , , , 405 408 and and CC 408 409 , , , 410 412 in in IN 413 423 particular particular JJ 423 424 , , , 425 428 the the DT 429 436 histone histone NN 437 439 H5 h5 NN 440 444 gene gene NN 444 445 . . . 446 451 Using use VBG 452 467 electrophoretic electrophoretic JJ 468 476 mobility mobility NN 477 482 shift shift NN 483 489 assays assay NNS 489 490 , , , 491 493 we we PRP 494 499 found find VBD 500 504 that that IN 505 507 in in IN 508 511 the the DT 512 520 presence presence NN 521 523 of of IN 524 530 rabbit rabbit NN 531 543 reticulocyte reticulocyte NN 544 550 lysate lysate NN 550 551 , , , 552 557 human human JJ 558 560 ER er NN 561 568 reduced reduce VBD 569 572 the the DT 573 580 binding binding NN 581 591 activities activity NNS 592 594 of of IN 595 602 chicken chicken NN 603 611 immature immature JJ 612 623 erythrocyte erythrocyte NN 624 631 nuclear nuclear JJ 632 641 extracted extracted JJ 642 650 proteins protein NNS 651 653 to to TO 654 658 GATA GATA NNP 659 662 and and CC 663 668 CACCC CACCC NNP 669 674 sites site NNS 675 677 in in IN 678 681 the the DT 682 684 H5 H5 NNP 685 693 promoter promoter NN 694 697 and and CC 698 706 enhancer enhancer NN 706 707 . . . 708 710 In in IN 711 719 contrast contrast NN 719 720 , , , 721 724 the the DT 725 732 binding binding NN 733 743 activities activity NNS 744 746 of of IN 747 750 NF1 NF1 NNP 751 754 and and CC 755 758 Sp1 Sp1 NNP 759 763 were be VBD 764 767 not not RB 768 776 affected affect VBN 777 779 by by IN 780 782 ER ER NNP 782 783 . . . 784 791 Binding binding NN 792 794 of of IN 795 797 ER er NN 798 800 to to TO 801 803 an an DT 804 812 estrogen estrogen NN 813 821 response response NN 822 829 element element NN 830 833 was be VBD 834 842 enhanced enhance VBN 843 845 by by IN 846 854 addition addition NN 855 857 of of IN 858 864 rabbit rabbit NN 865 877 reticulocyte reticulocyte NN 878 884 lysate lysate NN 884 885 . . . 886 890 This this DT 891 897 lysate lysate NN 898 901 was be VBD 902 906 also also RB 907 916 necessary necessary JJ 917 920 for for IN 921 923 ER ER NNP 924 926 to to TO 927 935 diminish diminish VB 936 939 the the DT 940 951 DNA-binding dna-binding JJ 952 960 activity activity NN 961 963 of of IN 964 970 GATA-1 gata-1 NN 970 971 . . . 972 977 These these DT 978 985 results result NNS 986 993 suggest suggest VBP 994 998 that that IN 999 1009 additional additional JJ 1010 1019 factor(s) factor(s) NNS 1020 1023 are be VBP 1024 1033 necessary necessary JJ 1034 1037 for for IN 1038 1042 full full JJ 1043 1045 ER er NN 1046 1054 function function NN 1054 1055 . . . 1056 1060 Both both CC 1061 1067 GATA-1 gata-1 NN 1068 1071 and and CC 1072 1085 CACCC-binding caccc-binding JJ 1086 1094 proteins protein NNS 1095 1098 are be VBP 1099 1107 critical critical JJ 1108 1111 for for IN 1112 1115 the the DT 1116 1131 developmentally developmentally RB 1132 1141 regulated regulate VBN 1142 1152 expression expression NN 1153 1155 of of IN 1156 1174 erythroid-specific erythroid-specific JJ 1175 1180 genes gene NNS 1180 1181 . . . 1182 1184 We we PRP 1185 1196 hypothesize hypothesize VBP 1197 1201 that that IN 1202 1214 interference interference NN 1215 1217 in in IN 1218 1229 DNA-binding dna-binding JJ 1230 1240 activities activity NNS 1241 1243 of of IN 1244 1250 GATA-1 gata-1 NN 1251 1254 and and CC 1255 1268 CACCC-binding caccc-binding JJ 1269 1277 proteins protein NNS 1278 1280 is be VBZ 1281 1284 the the DT 1285 1294 mechanism mechanism NN 1295 1297 by by IN 1298 1303 which which WDT 1304 1307 the the DT 1308 1310 ER er NN 1311 1319 inhibits inhibit VBZ 1320 1330 regulation regulation NN 1331 1333 of of IN 1334 1339 these these DT 1340 1345 genes gene NNS 1345 1346 . . .