0	10	Regulation	Regulation	NNP
11	13	of	of	IN
14	22	NF-kappa	NF-kappa	NNP
23	24	B	B	NNP
25	33	activity	activity	NN
34	36	by	by	IN
37	38	I	i	NN
39	44	kappa	kappa	NN
45	46	B	B	NNP
47	52	alpha	alpha	NN
53	56	and	and	CC
57	58	I	i	NN
59	64	kappa	kappa	NN
65	66	B	B	NNP
67	71	beta	beta	NN
72	81	stability	stability	NN
81	82	.	.	.

84	97	Transcription	Transcription	NNP
98	104	factor	factor	NN
105	113	NF-kappa	NF-kappa	NNP
114	115	B	B	NNP
116	120	must	must	MD
121	123	be	be	VB
124	132	released	release	VBN
133	137	from	from	IN
138	149	cytoplasmic	cytoplasmic	JJ
150	160	inhibitory	inhibitory	JJ
161	170	molecules	molecule	NNS
171	172	(	(	(
172	173	I	i	NN
174	179	kappa	kappa	NN
180	182	Bs	b	NNS
182	183	)	)	)
184	186	in	in	IN
187	192	order	order	NN
193	195	to	to	TO
196	200	move	move	VB
201	203	to	to	TO
204	207	the	the	DT
208	215	nucleus	nucleus	NN
216	219	and	and	CC
220	222	to	to	TO
223	231	activate	activate	VB
232	235	its	its	PRP$
236	242	target	target	NN
243	248	genes	gene	NNS
248	249	.	.	.

250	256	Little	little	JJ
257	259	is	be	VBZ
260	265	known	know	VBN
266	271	about	about	IN
272	275	the	the	DT
276	286	mechanisms	mechanism	NNS
287	297	regulating	regulate	VBG
298	301	the	the	DT
302	313	maintenance	maintenance	NN
314	316	of	of	IN
317	329	constitutive	constitutive	JJ
330	337	nuclear	nuclear	JJ
338	346	NF-kappa	NF-kappa	NNP
347	348	B	B	NNP
349	351	in	in	IN
352	356	some	some	DT
357	367	cell-types	cell-type	NNS
368	371	and	and	CC
372	374	of	of	IN
375	384	sustained	sustained	JJ
385	392	nuclear	nuclear	JJ
393	401	NF-kappa	NF-kappa	NNP
402	403	B	B	NNP
404	412	activity	activity	NN
413	418	after	after	IN
419	430	stimulation	stimulation	NN
430	431	.	.	.

432	441	Increased	increase	VBN
442	450	turnover	turnover	NN
451	454	has	have	VBZ
455	459	been	be	VBN
460	470	implicated	implicate	VBN
471	473	in	in	IN
474	477	the	the	DT
478	488	regulation	regulation	NN
489	491	of	of	IN
492	504	constitutive	constitutive	JJ
505	513	NF-kappa	NF-kappa	NNP
514	515	B	B	NNP
516	524	activity	activity	NN
525	527	in	in	IN
528	534	mature	mature	JJ
535	536	B	B	NNP
537	542	cells	cell	NNS
542	543	.	.	.

544	546	We	we	PRP
547	556	therefore	therefore	RB
557	565	compared	compare	VBD
566	569	the	the	DT
570	578	turnover	turnover	NN
579	581	of	of	IN
582	583	I	i	NN
584	589	kappa	kappa	NN
590	591	B	B	NNP
592	597	alpha	alpha	NN
598	601	and	and	CC
602	603	I	i	NN
604	609	kappa	kappa	NN
610	611	B	B	NNP
612	616	beta	beta	NN
617	619	in	in	IN
620	626	mature	mature	JJ
627	628	B	B	NNP
629	634	cells	cell	NNS
635	638	and	and	CC
639	643	HeLa	HeLa	NNP
644	649	cells	cell	NNS
649	650	.	.	.

651	655	Both	both	DT
656	664	proteins	protein	NNS
665	672	display	display	VBP
673	674	a	a	DT
675	679	high	high	JJ
680	688	turnover	turnover	NN
689	691	in	in	IN
692	693	B	B	NNP
694	699	cells	cell	NNS
700	708	although	although	IN
709	710	I	i	NN
711	716	kappa	kappa	NN
717	718	B	B	NNP
719	723	beta	beta	NN
724	726	is	be	VBZ
727	739	considerably	considerably	RB
740	744	more	more	RBR
745	751	stable	stable	JJ
752	756	than	than	IN
757	758	I	i	NN
759	764	kappa	kappa	NN
765	766	B	B	NNP
767	772	alpha	alpha	NN
772	773	.	.	.

774	777	The	the	DT
778	787	half-life	half-life	NN
788	790	of	of	IN
791	795	both	both	DT
796	806	inhibitors	inhibitor	NNS
807	809	is	be	VBZ
810	819	increased	increase	VBN
820	822	in	in	IN
823	827	HeLa	HeLa	NNP
828	833	cells	cell	NNS
833	834	.	.	.

835	837	In	in	IN
838	846	contrast	contrast	NN
846	847	,	,	,
848	851	all	all	DT
852	857	other	other	JJ
858	866	NF-kappa	NF-kappa	NNP
867	870	B/I	B/I	NNP
871	876	kappa	kappa	NN
877	878	B	B	NNP
879	888	molecules	molecule	NNS
889	895	tested	test	VBN
896	899	are	be	VBP
900	910	relatively	relatively	RB
911	917	stable	stable	JJ
918	920	in	in	IN
921	925	both	both	DT
926	936	cell-types	cell-type	NNS
936	937	.	.	.

938	941	The	the	DT
942	950	elevated	elevated	JJ
951	959	turnover	turnover	NN
960	962	of	of	IN
963	973	endogenous	endogenous	JJ
974	975	I	i	NN
976	981	kappa	kappa	NN
982	983	B	B	NNP
984	989	alpha	alpha	NN
990	992	in	in	IN
993	1000	Namalwa	Namalwa	NNP
1001	1006	cells	cell	NNS
1007	1009	is	be	VBZ
1010	1019	inhibited	inhibit	VBN
1020	1022	by	by	IN
1023	1024	a	a	DT
1025	1035	proteasome	proteasome	NN
1036	1045	inhibitor	inhibitor	NN
1046	1049	and	and	CC
1050	1054	thus	thus	RB
1055	1060	seems	seem	VBZ
1061	1063	to	to	TO
1064	1066	be	be	VB
1067	1073	driven	drive	VBN
1074	1076	by	by	IN
1077	1080	the	the	DT
1081	1085	same	same	JJ
1086	1097	degradation	degradation	NN
1098	1107	machinery	machinery	NN
1108	1110	as	as	IN
1111	1114	the	the	DT
1115	1121	slower	slow	JJR
1122	1130	turnover	turnover	NN
1131	1133	in	in	IN
1134	1139	non-B	non-b	JJ
1140	1145	cells	cell	NNS
1145	1146	.	.	.

1147	1158	Furthermore	furthermore	RB
1158	1159	,	,	,
1160	1162	we	we	PRP
1163	1175	investigated	investigate	VBD
1176	1179	the	the	DT
1180	1189	processes	process	NNS
1190	1198	involved	involve	VBN
1199	1201	in	in	IN
1202	1212	persistent	persistent	JJ
1213	1223	activation	activation	NN
1224	1226	of	of	IN
1227	1235	NF-kappa	NF-kappa	NNP
1236	1238	B.	B.	NNP
1239	1248	TNF-alpha	TNF-alpha	NNP
1249	1258	signaling	signaling	NN
1259	1264	leads	lead	VBZ
1265	1267	to	to	TO
1268	1269	a	a	DT
1270	1275	rapid	rapid	JJ
1276	1285	depletion	depletion	NN
1286	1288	of	of	IN
1289	1297	cellular	cellular	JJ
1298	1299	I	i	NN
1300	1305	kappa	kappa	NN
1306	1307	B	B	NNP
1308	1312	beta	beta	NN
1313	1318	pools	pool	NNS
1318	1319	.	.	.

1320	1321	I	i	NN
1322	1327	kappa	kappa	NN
1328	1329	B	B	NNP
1330	1335	alpha	alpha	NN
1336	1338	is	be	VBZ
1339	1350	efficiently	efficiently	RB
1351	1364	resynthesized	resynthesize	VBN
1365	1372	whereas	whereas	IN
1373	1374	I	i	NN
1375	1380	kappa	kappa	NN
1381	1382	B	B	NNP
1383	1387	beta	beta	NN
1388	1394	levels	level	NNS
1395	1399	stay	stay	VBP
1400	1403	low	low	JJ
1404	1407	for	for	IN
1408	1409	a	a	DT
1410	1419	prolonged	prolong	VBN
1420	1424	time	time	NN
1424	1425	.	.	.

1426	1434	NF-kappa	NF-kappa	NNP
1435	1436	B	B	NNP
1437	1444	binding	binding	NN
1445	1453	activity	activity	NN
1454	1457	can	can	MD
1458	1460	be	be	VB
1461	1469	detected	detect	VBN
1470	1473	for	for	IN
1474	1481	several	several	JJ
1482	1487	hours	hour	NNS
1488	1493	after	after	IN
1494	1505	stimulation	stimulation	NN
1505	1506	.	.	.

1507	1509	We	we	PRP
1510	1515	found	find	VBD
1516	1520	that	that	IN
1521	1528	removal	removal	NN
1529	1531	of	of	IN
1532	1535	the	the	DT
1536	1545	TNF-alpha	TNF-alpha	NNP
1546	1556	containing	contain	VBG
1557	1563	medium	medium	NN
1564	1570	causes	cause	VBZ
1571	1572	a	a	DT
1573	1578	rapid	rapid	JJ
1579	1587	decrease	decrease	NN
1588	1590	in	in	IN
1591	1598	nuclear	nuclear	JJ
1599	1607	NF-kappa	NF-kappa	NNP
1608	1610	B.	B.	NNP
1611	1612	A	a	NN
1613	1624	phosphoform	phosphoform	NN
1625	1627	of	of	IN
1628	1633	newly	newly	RB
1634	1645	synthesized	synthesize	VBN
1646	1647	I	i	NN
1648	1653	kappa	kappa	NN
1654	1655	B	B	NNP
1656	1661	alpha	alpha	NN
1662	1664	is	be	VBZ
1665	1672	visible	visible	JJ
1673	1677	when	when	WRB
1678	1689	degradation	degradation	NN
1690	1692	by	by	IN
1693	1696	the	the	DT
1697	1707	proteasome	proteasome	NN
1708	1710	is	be	VBZ
1711	1720	inhibited	inhibit	VBN
1721	1724	and	and	CC
1725	1728	new	new	JJ
1729	1730	I	i	NN
1731	1736	kappa	kappa	NN
1737	1738	B	B	NNP
1739	1744	alpha	alpha	NN
1745	1753	displays	display	VBZ
1754	1757	the	the	DT
1758	1762	same	same	JJ
1763	1773	properties	property	NNS
1774	1783	regarding	regard	VBG
1784	1799	phosphorylation	phosphorylation	NN
1800	1803	and	and	CC
1804	1815	degradation	degradation	NN
1816	1818	in	in	IN
1819	1827	response	response	NN
1828	1830	to	to	TO
1831	1832	a	a	DT
1833	1839	second	second	JJ
1840	1847	inducer	inducer	NN
1847	1848	.	.	.

1849	1854	There	there	EX
1855	1857	is	be	VBZ
1858	1860	no	no	DT
1861	1872	significant	significant	JJ
1873	1883	difference	difference	NN
1884	1886	in	in	IN
1887	1890	the	the	DT
1891	1899	turnover	turnover	NN
1900	1902	of	of	IN
1903	1907	pre-	pre-	JJ
1908	1911	and	and	CC
1912	1926	post-inductive	post-inductive	JJ
1927	1928	I	i	NN
1929	1934	kappa	kappa	NN
1935	1936	B	B	NNP
1937	1942	alpha	alpha	NN
1942	1943	.	.	.

1944	1949	These	these	DT
1950	1962	observations	observation	NNS
1963	1970	suggest	suggest	VBP
1971	1975	that	that	IN
1976	1987	resynthesis	resynthesis	NN
1988	1990	of	of	IN
1991	1992	I	i	NN
1993	1998	kappa	kappa	NN
1999	2000	B	B	NNP
2001	2006	alpha	alpha	NN
2007	2010	and	and	CC
2011	2018	removal	removal	NN
2019	2021	of	of	IN
2022	2025	the	the	DT
2026	2034	stimulus	stimulus	NN
2035	2038	are	be	VBP
2039	2049	obligatory	obligatory	JJ
2050	2055	steps	step	NNS
2056	2059	for	for	IN
2060	2063	the	the	DT
2064	2076	inactivation	inactivation	NN
2077	2079	of	of	IN
2080	2087	nuclear	nuclear	JJ
2088	2090	NF	NF	NNP
2091	2096	kappa	kappa	NN
2097	2098	B	B	NNP
2098	2099	.	.	.