0 7 Control Control NNP 8 10 of of IN 11 19 NF-kappa NF-kappa NNP 20 21 B B NNP 22 30 activity activity NN 31 33 by by IN 34 37 the the DT 38 39 I i NN 40 45 kappa kappa NN 46 47 B B NNP 48 52 beta beta NN 53 62 inhibitor inhibitor NN 62 63 . . . 65 68 The the DT 69 82 transcription transcription NN 83 89 factor factor NN 90 98 NF-kappa NF-kappa NNP 99 100 B B NNP 101 103 is be VBZ 104 114 maintained maintain VBN 115 117 in in IN 118 120 an an DT 121 129 inactive inactive JJ 130 141 cytoplasmic cytoplasmic JJ 142 147 state state NN 148 150 by by IN 151 152 I i NN 153 158 kappa kappa NN 159 160 B B NNP 161 171 inhibitors inhibitor NNS 171 172 . . . 173 175 In in IN 176 185 mammalian mammalian JJ 186 191 cells cell NNS 191 192 , , , 193 194 I i NN 195 200 kappa kappa NN 201 202 B B NNP 203 208 alpha alpha NN 209 212 and and CC 213 214 I i NN 215 220 kappa kappa NN 221 222 B B NNP 223 227 beta beta NN 228 236 proteins protein NNS 237 241 have have VBP 242 246 been be VBN 247 255 purified purify VBN 256 259 and and CC 260 265 shown show VBN 266 268 to to TO 269 271 be be VB 272 275 the the DT 276 286 inhibitors inhibitor NNS 287 289 of of IN 290 298 NF-kappa NF-kappa NNP 299 300 B B NNP 301 308 through through IN 309 314 their their PRP$ 315 326 association association NN 327 331 with with IN 332 335 the the DT 336 339 p65 p65 NN 340 342 or or CC 343 348 c-Rel c-rel NN 349 357 subunits subunit NNS 357 358 . . . 359 361 In in IN 362 370 addition addition NN 370 371 , , , 372 374 we we PRP 375 379 have have VBP 380 388 isolated isolate VBN 389 390 a a DT 391 396 third third JJ 397 405 NF-kappa NF-kappa NNP 406 407 B B NNP 408 417 inhibitor inhibitor NN 417 418 , , , 419 420 I i NN 421 426 kappa kappa NN 427 428 B B NNP 429 436 epsilon epsilon NN 437 438 ( ( ( 438 439 1 1 CD 439 440 ) ) ) 440 441 . . . 442 446 Upon upon IN 447 456 treatment treatment NN 457 461 with with IN 462 463 a a DT 464 469 large large JJ 470 477 variety variety NN 478 480 of of IN 481 489 inducers inducer NNS 489 490 , , , 491 492 I i NN 493 498 kappa kappa NN 499 500 B B NNP 501 506 alpha alpha NN 506 507 , , , 508 509 I i NN 510 515 kappa kappa NN 516 517 B B NNP 518 522 beta beta NN 523 526 are be VBP 527 542 proteolytically proteolytically RB 543 551 degraded degrade VBN 551 552 , , , 553 562 resulting result VBG 563 565 in in IN 566 574 NF-kappa NF-kappa NNP 575 576 B B NNP 577 590 translocation translocation NN 591 595 into into IN 596 599 the the DT 600 607 nucleus nucleus NN 607 608 . . . 609 613 Here here RB 614 616 we we PRP 617 621 show show VBP 622 626 that that IN 627 629 in in IN 630 635 E29.1 e29.1 NN 636 637 T t NN 638 642 cell cell NN 643 652 hybridoma hybridoma NN 653 654 I i NN 655 660 kappa kappa NN 661 662 B B NNP 663 668 alpha alpha NN 669 672 and and CC 673 674 I i NN 675 680 kappa kappa NN 681 682 B B NNP 683 687 beta beta NN 688 691 are be VBP 692 699 equally equally RB 700 710 associated associate VBN 711 715 with with IN 716 719 p65 p65 NN 720 723 and and CC 724 728 that that IN 729 730 I i NN 731 736 kappa kappa NN 737 738 B B NNP 739 743 beta beta NN 744 746 is be VBZ 747 755 degraded degrade VBN 756 758 in in IN 759 767 response response NN 768 770 to to TO 771 774 TNF TNF NNP 775 780 alpha alpha NN 781 783 in in IN 784 792 contrast contrast NN 793 795 to to TO 796 800 what what WP 801 804 has have VBZ 805 809 been be VBN 810 820 originally originally RB 821 830 published publish VBN 830 831 . . . 832 835 Our our PRP$ 836 840 data datum NNS 841 845 also also RB 846 853 suggest suggest VBP 854 858 that that IN 858 859 , , , 860 866 unlike unlike IN 867 868 I i NN 869 874 kappa kappa NN 875 876 B B NNP 877 882 alpha alpha NN 882 883 , , , 884 885 I i NN 886 891 kappa kappa NN 892 893 B B NNP 894 898 beta beta NN 899 901 is be VBZ 902 916 constitutively constitutively RB 917 931 phosphorylated phosphorylate VBN 932 935 and and CC 936 949 resynthesized resynthesize VBN 950 952 as as IN 953 954 a a DT 955 973 hypophosphorylated hypophosphorylate VBN 974 978 form form NN 978 979 . . . 980 983 The the DT 984 991 absence absence NN 992 994 of of IN 995 999 slow slow JJ 1000 1009 migrating migrating JJ 1010 1015 forms form NNS 1016 1018 of of IN 1019 1020 I i NN 1021 1026 kappa kappa NN 1027 1028 B B NNP 1029 1033 beta beta NN 1034 1043 following follow VBG 1044 1055 stimulation stimulation NN 1056 1064 suggests suggest VBZ 1065 1069 that that IN 1070 1073 the the DT 1074 1089 phosphorylation phosphorylation NN 1090 1094 does do VBZ 1095 1098 not not RB 1099 1110 necessarily necessarily RB 1111 1121 constitute constitute VB 1122 1125 the the DT 1126 1140 signal-induced signal-induced JJ 1141 1146 event event NN 1147 1152 which which WDT 1153 1160 targets target VBZ 1161 1164 the the DT 1165 1173 molecule molecule NN 1174 1177 for for IN 1178 1189 proteolysis proteolysis NN 1189 1190 . . .