0 8 NF-kappa NF-kappa NNP 9 14 B/Rel B/Rel NNP 15 21 family family NN 22 29 members member NNS 30 40 regulating regulate VBG 41 44 the the DT 45 51 ICAM-1 icam-1 NN 52 60 promoter promoter NN 61 63 in in IN 64 73 monocytic monocytic JJ 74 79 THP-1 thp-1 NN 80 85 cells cell NNS 85 86 . . . 88 89 A a DT 90 95 kappa kappa NN 96 102 B-site B-site NNP 103 106 was be VBD 107 117 identified identify VBN 118 120 in in IN 121 124 the the DT 125 133 promoter promoter NN 134 136 of of IN 137 140 the the DT 141 154 intercellular intercellular JJ 155 163 adhesion adhesion NN 164 174 molecule-1 molecule-1 NN 175 176 ( ( ( 176 182 ICAM-1 icam-1 NN 182 183 ) ) ) 184 188 gene gene NN 188 189 , , , 190 195 which which WDT 196 198 is be VBZ 199 207 involved involve VBN 208 210 in in IN 211 221 regulation regulation NN 222 224 of of IN 225 231 ICAM-1 icam-1 NN 232 242 expression expression NN 243 245 by by IN 246 251 tumor tumor NN 252 260 necrosis necrosis NN 261 267 factor factor NN 268 273 alpha alpha NN 274 275 ( ( ( 275 284 TNF-alpha TNF-alpha NNP 284 285 ) ) ) 286 289 and and CC 290 305 glucocorticoids glucocorticoid NNS 305 306 . . . 307 309 We we PRP 310 313 now now RB 314 320 report report VBP 321 323 on on IN 324 327 the the DT 328 341 transcription transcription NN 342 349 factors factor NNS 350 355 which which WDT 356 360 bind bind VBP 361 364 and and CC 365 378 transactivate transactivate VBP 379 383 this this DT 384 392 enhancer enhancer NN 393 401 sequence sequence NN 401 402 . . . 403 405 In in FW 406 411 vitro vitro FW 411 412 , , , 413 416 the the DT 417 423 ICAM-1 icam-1 NN 424 429 kappa kappa NN 430 431 B B NNP 432 436 site site NN 437 445 appeared appear VBD 446 448 to to TO 449 453 bind bind VB 454 458 RelA RelA NNP 459 462 and and CC 463 468 c-Rel c-rel NN 469 479 homodimers homodimer NNS 480 482 as as RB 483 487 well well RB 488 490 as as IN 491 503 heterodimers heterodimer NNS 504 508 with with IN 509 517 NF-kappa NF-kappa NNP 518 520 B1 B1 NNP 520 521 , , , 522 525 but but CC 526 532 weakly weakly RB 533 541 NF-kappa NF-kappa NNP 542 544 B1 B1 NNP 545 555 homodimers homodimer NNS 555 556 . . . 557 559 In in IN 560 568 addition addition NN 568 569 , , , 570 574 both both CC 575 579 RelA RelA NNP 580 583 and and CC 584 589 c-Rel c-rel NN 589 590 , , , 591 594 but but CC 595 598 not not RB 599 607 NF-kappa NF-kappa NNP 608 610 B1 B1 NNP 610 611 , , , 612 616 were be VBD 617 622 shown show VBN 623 625 to to TO 626 639 transactivate transactivate VB 640 642 an an DT 643 649 ICAM-1 icam-1 NN 650 655 kappa kappa NN 656 666 B-reporter B-reporter NNP 667 676 construct construct NN 676 677 . . . 678 680 In in IN 681 690 monocytic monocytic JJ 691 696 THP-1 thp-1 NN 697 702 cells cell NNS 703 712 TNF-alpha TNF-alpha NNP 713 720 induced induce VBD 721 724 two two CD 725 732 nuclear nuclear JJ 733 742 complexes complex NNS 743 748 which which WDT 749 751 in in FW 752 757 vitro vitro FW 758 763 bound bind VBD 764 766 to to TO 767 770 the the DT 771 777 ICAM-1 icam-1 NN 778 783 kappa kappa NN 784 785 B B NNP 786 790 site site NN 790 791 . . . 792 797 Using use VBG 798 808 antibodies antibody NNS 809 811 in in IN 812 814 an an DT 815 830 electrophoretic electrophoretic JJ 831 839 mobility mobility NN 840 850 supershift supershift NN 851 856 assay assay NN 856 857 , , , 858 861 one one CD 862 864 of of IN 865 870 these these DT 871 880 complexes complex NNS 881 884 was be VBD 885 890 shown show VBN 891 893 to to TO 894 901 contain contain VB 902 910 NF-kappa NF-kappa NNP 911 913 B1 B1 NNP 914 917 and and CC 918 922 RelA RelA NNP 922 923 , , , 924 927 and and CC 928 930 to to TO 931 935 bind bind VB 936 940 with with IN 941 947 higher high JJR 948 956 affinity affinity NN 957 959 to to TO 960 963 the the DT 964 973 consensus consensus NN 974 979 kappa kappa NN 980 981 B B NNP 982 986 site site NN 987 989 in in IN 990 993 the the DT 994 997 HIV HIV NNP 998 1002 long long JJ 1003 1011 terminal terminal JJ 1012 1018 repeat repeat NN 1018 1019 . . . 1020 1023 The the DT 1024 1030 second second JJ 1031 1038 complex complex NN 1039 1048 contained contain VBD 1049 1053 RelA RelA NNP 1053 1054 , , , 1055 1058 and and CC 1059 1068 exhibited exhibit VBD 1069 1075 higher high JJR 1076 1084 affinity affinity NN 1085 1092 towards towards IN 1093 1096 the the DT 1097 1103 ICAM-1 icam-1 NN 1104 1109 kappa kappa NN 1110 1111 B b NN 1112 1116 than than IN 1117 1119 to to TO 1120 1123 the the DT 1124 1127 HIV HIV NNP 1128 1133 kappa kappa NN 1134 1135 B B NNP 1136 1140 site site NN 1140 1141 . . . 1142 1145 The the DT 1146 1160 glucocorticoid glucocorticoid NN 1161 1169 receptor receptor NN 1170 1173 was be VBD 1174 1179 shown show VBN 1180 1182 to to TO 1183 1190 repress repress VB 1191 1199 activity activity NN 1200 1202 of of IN 1203 1207 both both CC 1208 1211 the the DT 1212 1216 RelA RelA NNP 1217 1226 homodimer homodimer NN 1227 1230 and and CC 1231 1234 the the DT 1235 1243 NF-kappa NF-kappa NNP 1244 1251 B1/RelA B1/RelA NNP 1252 1263 heterodimer heterodimer NN 1263 1264 . . . 1265 1267 We we PRP 1268 1273 argue argue VBP 1274 1278 that that IN 1279 1281 in in FW 1282 1286 vivo vivo FW 1287 1291 RelA RelA NNP 1292 1302 homodimers homodimer NNS 1303 1306 are be VBP 1307 1313 likely likely JJ 1314 1316 to to TO 1317 1321 play play VB 1322 1323 a a DT 1324 1332 dominant dominant JJ 1333 1337 role role NN 1338 1340 in in IN 1341 1358 TNF-alpha-induced tnf-alpha-induced JJ 1359 1365 ICAM-1 icam-1 NN 1366 1379 transcription transcription NN 1380 1382 in in IN 1383 1392 monocytic monocytic JJ 1393 1398 cells cell NNS 1398 1399 . . .