0	5	Human	human	JJ
6	22	immunodeficiency	immunodeficiency	NN
23	28	virus	virus	NN
29	33	type	type	NN
34	35	1	1	CD
36	40	long	long	JJ
41	49	terminal	terminal	JJ
50	56	repeat	repeat	NN
57	69	quasispecies	quasispecy	NNS
70	76	differ	differ	VBP
77	79	in	in	IN
80	85	basal	basal	JJ
86	99	transcription	transcription	NN
100	103	and	and	CC
104	111	nuclear	nuclear	JJ
112	118	factor	factor	NN
119	130	recruitment	recruitment	NN
131	133	in	in	IN
134	139	human	human	JJ
140	145	glial	glial	JJ
146	151	cells	cell	NNS
152	155	and	and	CC
156	167	lymphocytes	lymphocyte	NNS
167	168	.	.	.

170	173	The	the	DT
174	184	generation	generation	NN
185	187	of	of	IN
188	195	genomic	genomic	JJ
196	205	diversity	diversity	NN
206	212	during	during	IN
213	216	the	the	DT
217	223	course	course	NN
224	226	of	of	IN
227	236	infection	infection	NN
237	240	has	have	VBZ
241	244	the	the	DT
245	254	potential	potential	NN
255	257	to	to	TO
258	264	affect	affect	VB
265	268	all	all	DT
269	276	aspects	aspect	NNS
277	279	of	of	IN
280	285	HIV-1	HIV-1	NNP
286	297	replication	replication	NN
297	298	,	,	,
299	308	including	include	VBG
309	319	expression	expression	NN
320	322	of	of	IN
323	326	the	the	DT
327	335	proviral	proviral	JJ
336	342	genome	genome	NN
342	343	.	.	.

344	346	To	to	TO
347	351	gain	gain	VB
352	353	a	a	DT
354	360	better	good	JJR
361	374	understanding	understanding	NN
375	377	of	of	IN
378	381	the	the	DT
382	388	impact	impact	NN
389	391	of	of	IN
392	396	long	long	JJ
397	405	terminal	terminal	JJ
406	412	repeat	repeat	NN
413	414	(	(	(
414	417	LTR	LTR	NNP
417	418	)	)	)
419	427	sequence	sequence	NN
428	437	diversity	diversity	NN
438	440	on	on	IN
441	453	LTR-directed	ltr-directed	JJ
454	458	gene	gene	NN
459	469	expression	expression	NN
470	472	in	in	IN
473	478	cells	cell	NNS
479	481	of	of	IN
482	485	the	the	DT
486	493	central	central	JJ
494	501	nervous	nervous	JJ
502	508	system	system	NN
509	510	(	(	(
510	513	CNS	cns	NN
513	514	)	)	)
515	518	and	and	CC
519	525	immune	immune	JJ
526	532	system	system	NN
532	533	,	,	,
534	536	we	we	PRP
537	546	amplified	amplify	VBD
547	550	and	and	CC
551	557	cloned	clone	VBN
558	562	LTRs	ltr	NNS
563	567	from	from	IN
568	576	proviral	proviral	JJ
577	580	DNA	dna	NN
581	583	in	in	IN
584	598	HIV-1-infected	hiv-1-infected	JJ
599	609	peripheral	peripheral	JJ
610	615	blood	blood	NN
615	616	.	.	.

617	625	Sequence	sequence	NN
626	634	analysis	analysis	NN
635	637	of	of	IN
638	646	nineteen	nineteen	CD
647	651	LTRs	ltr	NNS
652	658	cloned	clone	VBN
659	663	from	from	IN
664	665	2	2	CD
666	671	adult	adult	JJ
672	675	and	and	CC
676	677	3	3	CD
678	687	pediatric	pediatric	JJ
688	696	patients	patient	NNS
697	705	revealed	reveal	VBD
706	708	an	an	DT
709	716	average	average	NN
717	719	of	of	IN
720	722	33	33	CD
723	733	nucleotide	nucleotide	NN
734	741	changes	change	NNS
742	743	(	(	(
743	747	with	with	IN
748	755	respect	respect	NN
756	758	to	to	TO
759	762	the	the	DT
763	771	sequence	sequence	NN
772	774	of	of	IN
775	778	the	the	DT
779	782	LAI	LAI	NNP
783	786	LTR	LTR	NNP
786	787	)	)	)
788	794	within	within	IN
795	798	the	the	DT
799	805	455-bp	455-bp	JJ
806	808	U3	u3	NN
809	815	region	region	NN
815	816	.	.	.

817	826	Transient	transient	JJ
827	837	expression	expression	NN
838	846	analyses	analysis	NNS
847	849	in	in	IN
850	855	cells	cell	NNS
856	858	of	of	IN
859	869	neuroglial	neuroglial	JJ
870	873	and	and	CC
874	885	lymphocytic	lymphocytic	JJ
886	892	origin	origin	NN
893	905	demonstrated	demonstrate	VBD
906	910	that	that	IN
911	915	some	some	DT
916	918	of	of	IN
919	924	these	these	DT
925	929	LTRs	LTR	NNPS
930	933	had	have	VBD
934	944	activities	activity	NNS
945	950	which	which	WDT
951	957	varied	vary	VBD
958	971	significantly	significantly	RB
972	976	from	from	IN
977	980	the	the	DT
981	984	LAI	LAI	NNP
985	988	LTR	LTR	NNP
989	991	in	in	IN
992	997	U-373	U-373	NNP
998	1000	MG	MG	NNP
1001	1006	cells	cell	NNS
1007	1008	(	(	(
1008	1010	an	an	DT
1011	1022	astrocytoma	astrocytoma	NN
1023	1027	cell	cell	NN
1028	1032	line	line	NN
1032	1033	)	)	)
1034	1036	as	as	RB
1037	1041	well	well	RB
1042	1044	as	as	IN
1045	1047	in	in	IN
1048	1054	Jurkat	Jurkat	NNP
1055	1060	cells	cell	NNS
1061	1062	(	(	(
1062	1063	a	a	DT
1064	1076	CD4-positive	cd4-positive	JJ
1077	1087	lymphocyte	lymphocyte	NN
1088	1092	cell	cell	NN
1093	1097	line	line	NN
1097	1098	)	)	)
1098	1099	.	.	.

1100	1105	While	while	IN
1106	1110	LTRs	LTR	NNP
1111	1116	which	which	WDT
1117	1129	demonstrated	demonstrate	VBD
1130	1133	the	the	DT
1134	1141	highest	high	JJS
1142	1152	activities	activity	NNS
1153	1155	in	in	IN
1156	1161	U-373	U-373	NNP
1162	1164	MG	MG	NNP
1165	1170	cells	cell	NNS
1171	1175	also	also	RB
1176	1183	yielded	yield	VBD
1184	1188	high	high	JJ
1189	1199	activities	activity	NNS
1200	1202	in	in	IN
1203	1209	Jurkat	Jurkat	NNP
1210	1215	cells	cell	NNS
1215	1216	,	,	,
1217	1220	the	the	DT
1221	1225	LTRs	ltr	NNS
1226	1230	were	be	VBD
1231	1240	generally	generally	RB
1241	1245	more	more	RBR
1246	1252	active	active	JJ
1253	1255	in	in	IN
1256	1262	Jurkat	Jurkat	NNP
1263	1268	cells	cell	NNS
1269	1273	when	when	WRB
1274	1282	compared	compare	VBN
1283	1285	to	to	TO
1286	1289	the	the	DT
1290	1293	LAI	LAI	NNP
1294	1297	LTR	LTR	NNP
1297	1298	.	.	.

1299	1310	Differences	difference	NNS
1311	1313	in	in	IN
1314	1317	LTR	LTR	NNP
1318	1326	sequence	sequence	NN
1327	1331	also	also	RB
1332	1340	resulted	result	VBD
1341	1343	in	in	IN
1344	1355	differences	difference	NNS
1356	1358	in	in	IN
1359	1372	transcription	transcription	NN
1373	1379	factor	factor	NN
1380	1391	recruitment	recruitment	NN
1392	1394	to	to	TO
1395	1405	cis-acting	cis-acting	JJ
1406	1411	sites	site	NNS
1412	1418	within	within	IN
1419	1422	the	the	DT
1423	1425	U3	u3	NN
1426	1432	region	region	NN
1433	1435	of	of	IN
1436	1439	the	the	DT
1440	1443	LTR	ltr	NN
1443	1444	,	,	,
1445	1447	as	as	IN
1448	1460	demonstrated	demonstrate	VBN
1461	1463	by	by	IN
1464	1479	electrophoretic	electrophoretic	JJ
1480	1488	mobility	mobility	NN
1489	1494	shift	shift	NN
1495	1501	assays	assay	NNS
1501	1502	.	.	.

1503	1505	In	in	IN
1506	1516	particular	particular	JJ
1516	1517	,	,	,
1518	1527	naturally	naturally	RB
1528	1537	occurring	occur	VBG
1538	1546	sequence	sequence	NN
1547	1556	variation	variation	NN
1557	1565	impacted	impact	VBD
1566	1579	transcription	transcription	NN
1580	1586	factor	factor	NN
1587	1594	binding	bind	VBG
1595	1597	to	to	TO
1598	1600	an	an	DT
1601	1611	activating	activate	VBG
1612	1625	transcription	transcription	NN
1626	1637	factor/cAMP	factor/camp	NN
1638	1646	response	response	NN
1647	1654	element	element	NN
1655	1662	binding	binding	NN
1663	1664	(	(	(
1664	1672	ATF/CREB	ATF/CREB	NNP
1672	1673	)	)	)
1674	1681	binding	binding	NN
1682	1686	site	site	NN
1687	1688	(	(	(
1688	1695	located	located	JJ
1696	1703	between	between	IN
1704	1707	the	the	DT
1708	1713	LEF-1	lef-1	NN
1714	1717	and	and	CC
1718	1724	distal	distal	JJ
1725	1734	NF-kappaB	NF-kappaB	NNP
1735	1748	transcription	transcription	NN
1749	1755	factor	factor	NN
1756	1763	binding	binding	NN
1764	1769	sites	site	NNS
1769	1770	)	)	)
1771	1775	that	that	WDT
1776	1778	we	we	PRP
1779	1789	identified	identify	VBD
1790	1792	in	in	IN
1793	1801	previous	previous	JJ
1802	1809	studies	study	NNS
1810	1812	of	of	IN
1813	1816	the	the	DT
1817	1822	HIV-1	HIV-1	NNP
1823	1826	LTR	LTR	NNP
1826	1827	.	.	.

1828	1833	These	these	DT
1834	1842	findings	finding	NNS
1843	1850	suggest	suggest	VBP
1851	1855	that	that	IN
1856	1859	LTR	LTR	NNP
1860	1868	sequence	sequence	NN
1869	1876	changes	change	NNS
1877	1880	can	can	MD
1881	1894	significantly	significantly	RB
1895	1901	affect	affect	VB
1902	1907	basal	basal	JJ
1908	1911	LTR	ltr	NN
1912	1920	function	function	NN
1921	1924	and	and	CC
1925	1938	transcription	transcription	NN
1939	1945	factor	factor	NN
1946	1957	recruitment	recruitment	NN
1957	1958	,	,	,
1959	1964	which	which	WDT
1965	1968	may	may	MD
1968	1969	,	,	,
1970	1972	in	in	IN
1973	1977	turn	turn	NN
1977	1978	,	,	,
1979	1984	alter	alter	VBP
1985	1988	the	the	DT
1989	1995	course	course	NN
1996	1998	of	of	IN
1999	2004	viral	viral	JJ
2005	2016	replication	replication	NN
2017	2019	in	in	IN
2020	2025	cells	cell	NNS
2026	2028	of	of	IN
2029	2032	CNS	cns	NN
2033	2036	and	and	CC
2037	2043	immune	immune	JJ
2044	2050	system	system	NN
2051	2057	origin	origin	NN
2057	2058	.	.	.